Agronomic performance and molecular assessment of tissue culture‐derived barley lines

Abstract

In order to assess the genetic variability among regenerants. field trials were conducted with 64 barley lines derived from tissue culture (TCD lines) of four Bulgarian barley varieties. The results indicate that the agronomic performance was altered by tissue culture regeneration and the frequency of variation was cultivar dependent. Seven TCD lines showing desirable agronomic characters were selected in the SC16 progeny. Molecular markers (protein, restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) have used applied to determine the variability induced by tissue culture. Polymorphism was detected in sequences coding for C‐hordeins in line 70 derived from cv.‘Jubiley’Two lines from cv.‘Ruen’exhibited polymorphic bands after hybridization with a mitochondrial DNA probe. RAPD assays have been carried out using 20 different l0‐mer primers. Heritable polymorphisms in several TCD lines have been observed.

Genetic instability of sugarcane plants derived from meristem cultures

“…Of the available techniques, RAPD is among the most useful ones (Rani et al, 1995;Taylor et al, 1995;Shoyama et al, 1997;Todoroviska et al, 1997;Rout et al, 1998). Changes in the RAPD pattern may result from the loss/gain of a primer annealing, caused by point mutations or by the insertion or deletion of sequences or transposition elements (Peschke et al, 1991).…”

Assessment of Genetic Polymorphism in Sugarcane Somaclones by Using Random Amplified Polymorphic DNA

“…Many workers widely used these techniques to detect and characterize somaclonal variation at the DNA level (Ford-Lloyd et al, 1992;Cloutier and Landry, 1994;Barrett et al, 1997). It was opined that among available techniques, RAPD was the most useful (Rani et al, 1995;Taylor et al, 1995;Shoyama et al, 1997;Todoroviska et al, 1997 andRout et al, 1998). Changes in the RAPD pattern may result from the loss/gain of a primer annealing, caused by point mutations or by the insertion or deletion of sequences or transposition elements (Peschke et al, 1991).…”

Biotechnological Approaches for Cereal Crops Improvement

“…Overall a total of 15 polymorphic phenotypes were found among Bulgarian cultivars using 11 out of 25 tested RAPD primers (70). RAPD assay proved also to be more useful in determination the genetic variability induced in tissue culture derived (TCD) lines of barley (69). Tissue culture method was chosen as one of the approaches applied for diversification of elite material in cereals.…”

“…Since such polymorphism has been found between lines with the value of r as high as 0.92, RAPD polymorphisms might be useful in distinguishing among barley lines that share a high degree of similarity. RFLPs in combination with RAPDs and biochemical markers have been used for genotyping and studying the genetic diversity of Bulgarian barley cultivars and their somaclonal variants (69,70 studied Bulgarian cultivars (0.93) was obtained based on both RFLP and RAPD markers. The study showed that RAPD markers are more suitable for discrimination of elite barley varieties with high coancestry.…”