Ah-receptor-dependent modulation of gene expression by agedand diluted sidestream cigarette smoke

Gebremichael, A.; Tullis, Kathryn; Denison, Michael S.; Cheek, Jeffrey M.; Pinkerton, Kent E.

doi:10.1016/s0041-008x(96)80011-1

Cited by 44 publications

(2 citation statements)

References 38 publications

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“…The strong induced band shown in Figure 6 represents the formation of an APM-inducible AhR-ARNT/DRE complex. These results are consistent with previous studies investigating the ability of complex mixtures to induce the formation of these complexes (30,65,66). The specificity of AhR/ARNT:DRE binding was demonstrated by the ability of (i) excess (25-fold) unlabeled DRE to outcompete the [γ 32 P]-labeled DRE (lanes 3, 6, and 9) and (ii) the inability of the point-mutated DRE to compete with the [γ 32 P]-labeled DRE (lanes 4, 7, and 10), thereby maintaining the strong AhR/ARNT:DRE induced band.…”

Section: Resultssupporting

confidence: 93%

Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM₁₀ Air Particulate Material Using in Vitro Gene Expression Assays

Clemons

Allan

Marvin

et al. 1998

Environ. Sci. Technol.

185

134

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Polar and nonpolar fractions prepared from an organic extract of inhalable air particulate material collected from an urban location in downtown Toronto, Ontario, Canada, were examined for estrogen and Ah receptor-mediated activities using in vitro gene expression assays. The presence of estrogenic activity was determined using MCF-7 human breast cancer cells transiently transfected with a Gal4-human estrogen receptor chimera and a Gal4-regulated luciferase reporter gene. 2,3,7,8-Tetracholordibenzo-p-dioxin (TCDD)-like activity was detected using Hepa 1c1c7 cells transiently transfected with a CYP1A1-regulated reporter gene (pGudLuc 1.1). Significant estrogenic and TCDD-like activity was detected in the crude extract and in the nonpolar fractions. Results from the analyses of nine environmentally prevalent polyaromatic hydrocarbons (PAH) indicated that PAH might be significant contributors to the observed activity. Surprisingly, three PAH, namely benzo[a]pyrene, chrysene, and benz[a]anthracene, were found to substantially induce in vitro estrogenic and TCDD-like activities that were mediated by the estrogen and Ah receptors, respectively. Benzo[k]fluoranthene, dibenz[a,h]anthracene, and anthracene also exhibited significant in vitro TCDD-like activity. These results demonstrate the utility of in vitro gene expression assays to identify the presence of potential endocrine disruptors within complex mixtures.

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Section: Resultssupporting

confidence: 93%

Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM₁₀ Air Particulate Material Using in Vitro Gene Expression Assays

Clemons

Allan

Marvin

et al. 1998

Environ. Sci. Technol.

185

134

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“…The CYP1 (cytochrome P450 1) family has two significant subtypes, CYP1A1 and CYP1B1, which are abundant in lung tissues. CYP1A1 and CYP1B1 participate in the metabolism of lung polycyclic aromatic hydrocarbons (PAHs) as PAHs-sensitive genes and can be activated by PAHs in the lung through the aryl hydrocarbon receptor (AhR) [34][35][36] . CYP1A1 is most abundant in alveolar type II cells and endothelial cells 37 , while CYP1B1 is most abundant in airway epithelial cells 35 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics profile of human bronchial epithelial cells exposed to ambient fine particles and influenza virus (H3N2)

Liu,

Wang,

Zhang

et al. 2023

Sci Rep

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Fine particulate matter (PM2.5) pollution remains a major threat to public health. As the physical barrier against inhaled air pollutants, airway epithelium is a primary target for PM2.5 and influenza viruses, two major environmental insults. Recent studies have shown that PM2.5 and influenza viruses may interact to aggravate airway inflammation, an essential event in the pathogenesis of diverse pulmonary diseases. Airway epithelium plays a critical role in lung health and disorders. Thus far, the mechanisms for the interactive effect of PM2.5 and the influenza virus on gene transcription of airway epithelial cells have not been fully uncovered. In this present pilot study, the transcriptome sequencing approach was introduced to identify responsive genes following individual and co-exposure to PM2.5 and influenza A (H3N2) viruses in a human bronchial epithelial cell line (BEAS-2B). Enrichment analysis revealed the function of differentially expressed genes (DEGs). Specifically, the DEGs enriched in the xenobiotic metabolism by the cytochrome P450 pathway were linked to PM2.5 exposure. In contrast, the DEGs enriched in environmental information processing and human diseases, such as viral protein interaction with cytokines and cytokine receptors and epithelial cell signaling in bacterial infection, were significantly related to H3N2 exposure. Meanwhile, co-exposure to PM2.5 and H3N2 affected G protein-coupled receptors on the cell surface. Thus, the results from this study provides insights into PM2.5- and influenza virus-induced airway inflammation and potential mechanisms.

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Effect of in utero and postnatal exposure to environmental tobacco smoke on the developmental expression of pulmonary cytochrome P450 monooxygenases

Lee

Royce

Denison

et al. 2000

J. Biochem. Mol. Toxicol.

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Pulmonary cytochrome P450 monooxygenases metabolize xenobiotic chemicals, including those found in environmental tobacco smoke (ETS). Exposure to ETS beginning at birth has been shown to induce the P450 CYP1A1 by seven days of life. The effects of perinatal exposure to ETS of the rat lung on the expression of CYP1A1, 1B1, 2B1, and NADPH cytochrome P450 reductase were measured using semiquantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR). Timed pregnant dams and their pups were exposed to aged and diluted sidestream cigarette smoke (ADSS) as a surrogate for ETS for four hours/day from gestational day 5 through postnatal day 21. For all genes analyzed, mRNA could be detected in the fetal lung beginning at gestational day 17 but were not altered by ADSS. In contrast, intraperitoneal injection of dams with β‐naphthoflavone significantly elevated both CYP1A1 and 1B1 at gestational day 21, indicating that these genes are inducible. Continued exposure to ADSS significantly induced CYP1A1 but not other P450 genes as early as one day after birth.. We conclude that (1) ADSS induces pulmonary CYP1A1 in the first day of life; (2) fetal cytochrome P450 genes are not induced by maternal exposure to ADSS; and (3) in the fetal lung, CYP1A1 and 1B1 can be induced by β‐naphthoflavone. © 2000 John Wiley & Sons, Inc. J Biochem Toxicol 14:121–130, 2000

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Ah-receptor-dependent modulation of gene expression by agedand diluted sidestream cigarette smoke

Cited by 44 publications

References 38 publications

Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM₁₀ Air Particulate Material Using in Vitro Gene Expression Assays

Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM₁₀ Air Particulate Material Using in Vitro Gene Expression Assays

Transcriptomics profile of human bronchial epithelial cells exposed to ambient fine particles and influenza virus (H3N2)

Effect of in utero and postnatal exposure to environmental tobacco smoke on the developmental expression of pulmonary cytochrome P450 monooxygenases

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Ah-receptor-dependent modulation of gene expression by agedand diluted sidestream cigarette smoke

Cited by 44 publications

References 38 publications

Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM10 Air Particulate Material Using in Vitro Gene Expression Assays

Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM10 Air Particulate Material Using in Vitro Gene Expression Assays

Transcriptomics profile of human bronchial epithelial cells exposed to ambient fine particles and influenza virus (H3N2)

Effect of in utero and postnatal exposure to environmental tobacco smoke on the developmental expression of pulmonary cytochrome P450 monooxygenases

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Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM₁₀ Air Particulate Material Using in Vitro Gene Expression Assays

Evidence of Estrogen- and TCDD-Like Activities in Crude and Fractionated Extracts of PM₁₀ Air Particulate Material Using in Vitro Gene Expression Assays