AhR2-mediated, CYP1A-independent cardiovascular toxicity in zebrafish (Danio rerio) embryos exposed to retene

Scott, Jason; Incardona, John P.; Pelkki, Kathleen L.; Shepardson, Sally; Hodson, Peter V.

doi:10.1016/j.aquatox.2010.09.016

Cited by 121 publications

(83 citation statements)

References 35 publications

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“…In zebrafish embryos, the effects of two geologically distinct oils (Alaskan and Iranian) were indistinguishable irrespective of whether PAHs originated from oiled gravel columns or HEWAFs (17,21). The similarity of the injury phenotype observed here in pelagic embryos and larvae, in the presence or absence of a broadspectrum antibiotic, provides further evidence that crude oil cardiotoxicity to fish embryos is both independent of exposure methodology and consistent with direct actions of individual PAH compounds on the developing fish heart (17,18,43).…”

Section: Discussionmentioning

confidence: 50%

“…The AHR is the ligand-activated transcription factor that induces the upregulation of protective PAH-metabolizing enzymes (e.g., cytochrome P4501A, CYP1A). Inappropriate AHR activation in heart muscle cells also underlies the cardiotoxicity of some higher molecular weight PAHs, as well as dioxins and polychlorinated biphenyls (43)(44)(45)(46)(47). Species-specific developmental variation in ion channel expression and other targets for PAHs in cardiac tissues may underlie the observed subtle differences in MC252 oil-induced functional defects in the developing hearts of tunas, amberjack, and zebrafish.…”

Section: Discussionmentioning

confidence: 99%

“…Crude oil HEWAFs similar to those used here were shown to block key voltage-gated potassium and calcium ion channels controlling excitation-contraction coupling in fish cardiomyocytes (22), and the precise impacts on cardiac function may depend on the relative levels of these targets in different species. In addition, results from studies on alkylated phenanthrenes and more weathered crude oil mixtures enriched with higher levels of alkylated tricyclic PAHs suggest mechanisms of cardiotoxicity that are downstream of aryl hydrocarbon receptor (AHR) activation in cardiac muscle cells (21,43). The AHR is the ligand-activated transcription factor that induces the upregulation of protective PAH-metabolizing enzymes (e.g., cytochrome P4501A, CYP1A).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Deepwater Horizon crude oil impacts the developing hearts of large predatory pelagic fish

Incardona

Gardner

Linbo

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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376

238

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The Deepwater Horizon disaster released more than 636 million L of crude oil into the northern Gulf of Mexico. The spill oiled upper surface water spawning habitats for many commercially and ecologically important pelagic fish species. Consequently, the developing spawn (embryos and larvae) of tunas, swordfish, and other large predators were potentially exposed to crude oil-derived polycyclic aromatic hydrocarbons (PAHs). Fish embryos are generally very sensitive to PAH-induced cardiotoxicity, and adverse changes in heart physiology and morphology can cause both acute and delayed mortality. Cardiac function is particularly important for fast-swimming pelagic predators with high aerobic demand. Offspring for these species develop rapidly at relatively high temperatures, and their vulnerability to crude oil toxicity is unknown. We assessed the impacts of field-collected Deepwater Horizon (MC252) oil samples on embryos of three pelagic fish: bluefin tuna, yellowfin tuna, and an amberjack. We show that environmentally realistic exposures (1-15 μg/L total PAH) cause specific dosedependent defects in cardiac function in all three species, with circulatory disruption culminating in pericardial edema and other secondary malformations. Each species displayed an irregular atrial arrhythmia following oil exposure, indicating a highly conserved response to oil toxicity. A considerable portion of Gulf water samples collected during the spill had PAH concentrations exceeding toxicity thresholds observed here, indicating the potential for losses of pelagic fish larvae. Vulnerability assessments in other ocean habitats, including the Arctic, should focus on the developing heart of resident fish species as an exceptionally sensitive and consistent indicator of crude oil impacts.oil spill | damage assessment | heart development | embryology

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Deepwater Horizon crude oil impacts the developing hearts of large predatory pelagic fish

Incardona

Gardner

Linbo

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

376

238

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“…edemas, changes in progeny, neuronal cell death, failed inflation of the swim bladder and anemia [14][15][16]. Alkylphenanthrenes are PAH constituents of crude oil and have been implicated in the toxicity of crude oil es-pecially to the early states [17]. PAH toxicity is linked to aryl hydrocarbon receptor (AhR) and alkylphenanthrenes such as retene are shown to produce AhR-dependent cardiac toxicity [17].…”

Section: Introductionmentioning

confidence: 99%

“…Alkylphenanthrenes are PAH constituents of crude oil and have been implicated in the toxicity of crude oil es-pecially to the early states [17]. PAH toxicity is linked to aryl hydrocarbon receptor (AhR) and alkylphenanthrenes such as retene are shown to produce AhR-dependent cardiac toxicity [17]. Crude oil exposure has been reported to shorten the hatching time of Pacific herring [9,13], while according to Lindén [11] the mortality of Baltic herring during oil exposure is highest immediately after hatching.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of Different Early Developmental Periods of Baltic Herring (Clupea harengus) to Water-accommodated Fraction of Crude Oil

Venesjärvi¹

2013

TOEPTJ

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The probability for the oil accident to occur at the Gulf of Finland has been increasing last ten years and it is predicted to continue so. The risk is high based on the accident probability and the probable harm caused; some spawning areas of the Baltic herring (Clupea harengus membras) are located in the vicinity of ports and ship fairways with heavy traffic, so any oil spillage there could affect recruitment of this commercially important species. In this study, newly fertilized embryos, the older embryos and the hatched larvae of Baltic herring were exposed to water-accommodated fraction (WAF) made from crude oil. Oil exposure increased most the mortality of the hatched larvae, and larvae hatched during the exposure were more sensitive to the impacts of oil than larvae which were exposed after their hatching. The LC 50 value was 16 % of WAF-solution of crude oil for the exposed larvae. The most tolerant for the oil were the embryos in late embryonic stage (the LC 50 was 44 % of WAF-solution). During the exposure, some malformations i.e. a curving of the notochord were noticed.

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Developmental toxicity screening in zebrafish

McCollum

Ducharme

Bondesson

et al. 2011

Birth Defects Research Part C: Embryo Today: Reviews

140

101

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Given the ever-increasing toxic exposure ubiquitously present in our environment as well as emerging evidence that these exposures are hazardous to human health, the current rodent-based regulations are proving inadequate. In the process of overhauling risk assessment methodology, a nonrodent test organism, the zebrafish, is emerging as tractable for medium- and high-throughput assessments, which may help to accelerate the restructuring of standards. Zebrafish have high developmental similarity to mammals in most aspects of embryo development, including early embryonic processes, and on cardiovascular, somite, muscular, skeletal, and neuronal systems. Here, we briefly describe the development of these systems and then chronicle the toxic impacts assessed following chemical exposure. We also compare the available data in zebrafish toxicity assays with two databases containing mammalian toxicity data. Finally, we identify gaps in our collective knowledge that are ripe for future studies.

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AhR2-mediated, CYP1A-independent cardiovascular toxicity in zebrafish (Danio rerio) embryos exposed to retene

Cited by 121 publications

References 35 publications

Deepwater Horizon crude oil impacts the developing hearts of large predatory pelagic fish

Deepwater Horizon crude oil impacts the developing hearts of large predatory pelagic fish

Sensitivity of Different Early Developmental Periods of Baltic Herring (Clupea harengus) to Water-accommodated Fraction of Crude Oil

Developmental toxicity screening in zebrafish

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