AHSG, a Gene Promoting Tumour Proliferation, Migration and Invasion, is an Independent Prognostic Factor for Poor Overall Survival in Lung Adenocarcinoma

Xing, Xiaoying; Cao, Fu-min; Gao, Liping; Song, Minglei

doi:10.1007/s11033-023-08623-x

Cited by 2 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ALB is an important transport protein in serum, and decreased ALB expression worsens the prognosis of CRC patients [33] . AHSG is a serum glycoprotein synthesized by hepatocytes and adipocytes that can promote tumor proliferation, migration, and invasion [34] . FGG is a brinogen component that plays an instrumental role in blood coagulation, brinolysis, and cell-matrix interactions.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis reveals link between alternative complement cascade pathway and colorectal cancer liver metastasis

Shi,

Li,

Wei

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Colorectal cancer (CRC) has a high incidence mortality rate and is characterized by liver metastasis, which is the main cause of CRC patient death. In this study, a transcriptome sequencing dataset (GSE81558) from the integrated Gene Expression Omnibus database was evaluated to gain new insights into the pathogenesis of CRC and potential therapeutic targets. Methods All raw data were processed using R and screened for differentially expressed genes (DEGs) using LIMMA software. In-depth Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted and visualized using R and Cytoscape software. Protein‒protein interactions (PPIs) associated with the DEGs were assessed using the Search Tool for the Retrieval of Interacting Genes/Proteins database. A mouse model of CRC liver metastasis of CRC was used to verify key associated signaling pathways. Results The GO biological processes (BPs) and KEGG pathway analyses revealed that DEGs between the normal colon and CRC samples were mainly involved in the cell cycle and the P53 signaling pathway, which regulate the cell cycle and alter tumor signaling pathways. The 10 hub genes identified by PPI were cell cycle-related. In CRC versus and CRC liver metastasis samples, the GO BPs were mainly associated with platelets and coagulation, and the KEGG pathways were mainly enriched in the complement and coagulation cascades and drug metabolism. The PPI hub genes were blood protein-related, such as ALB, AHSG, and APOH, or plasma protease inhibitors, such as SERPINC1. To confirm bioinformatics analysis results, we used wild-type (WT), C4 (an important molecule in the classical and lectin complement cascade pathways), and complement factor B (fB, an important molecule in the alternative complement cascade pathway) knockout (KO) mice to construct a CRC liver metastasis model. Compared with WT mice, fB-KO mice demonstrated significantly reduced liver metastasis and inflammation, while there was no difference in C4-KO mice. Conclusion Bioinformatics analyses revealed that the complement cascade is related to CRC liver metastasis and that the cell cycle is related to CRC. The role of the alternative complement pathway in CRC liver metastasis was confirmed in mice, indicating that this pathway is a potential therapeutic target in CRC liver metastasis and providing a theoretical basis for further research.

show abstract

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis reveals link between alternative complement cascade pathway and colorectal cancer liver metastasis

Shi,

Li,

Wei

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Differential expression of ST6GALNAC1 and ST6GALNAC2 and their clinical relevance to colorectal cancer progression

Ahmad,

Braoudaki,

Siddiqui

2024

PLoS ONE

View full text Add to dashboard Cite

Colorectal cancer (CRC) has become a significant global health concern and ranks among the leading causes of morbidity and mortality worldwide. Due to its malignant nature, current immunotherapeutic treatments are used to tackle this issue. However, not all patients respond positively to treatment, thereby limiting clinical effectiveness and requiring the identification of novel therapeutic targets to optimise current strategies. The putative ligand of Siglec-15, Sialyl-Tn (STn), is associated with tumour progression and is synthesised by the sialyltransferases ST6GALNAC1 and ST6GALNAC2. However, the deregulation of both sialyltransferases within the literature remain limited, and the involvement of microRNAs (miRNAs) in STn production require further elucidation. Here, we identified miRNAs involved in the regulation of ST6GALNAC1 via a computational approach and further analysis of miRNA binding sites were determined. In silico tools predicted miR-21, miR-30e and miR-26b to regulate the ST6GALNAC1 gene, all of which had shown significant upregulated expression in the tumour cohort. Moreover, each miRNA displayed a high binding affinity towards the seed region of ST6GALNAC1. Additionally, enrichment analysis outlined pathways associated with several cancer hallmarks, including epithelial to mesenchymal transition (EMT) and MYC targets associated with tumour progression. Furthermore, our in silico findings demonstrated that the ST6GALNAC1 expression profile was significantly downregulated in CRC tumours, and its low expression correlated with poor survival outcomes when compared with patient survival data. In comparison to its counterpart, there were no significant differences in the expression of ST6GALNAC2 between normal and malignant tissues, which was further evidenced in our immunohistochemistry analysis. Immunohistochemistry staining highlighted significantly higher expression was more prevalent in normal human tissues with regard to ST6GALNAC1. In conclusion, the integrated in silico analysis highlighted that STn production is not reliant on deregulated sialyltransferase expression in CRC, and ST6GALNAC1 expression is regulated by several oncomirs. We proposed the involvement of other sialyltransferases in the production of the STn antigen and CRC progression via the Siglec-15/Sia axis.

show abstract

AHSG, a Gene Promoting Tumour Proliferation, Migration and Invasion, is an Independent Prognostic Factor for Poor Overall Survival in Lung Adenocarcinoma

Cited by 2 publications

References 27 publications

Bioinformatics analysis reveals link between alternative complement cascade pathway and colorectal cancer liver metastasis

Bioinformatics analysis reveals link between alternative complement cascade pathway and colorectal cancer liver metastasis

Differential expression of ST6GALNAC1 and ST6GALNAC2 and their clinical relevance to colorectal cancer progression

Contact Info

Product

Resources

About