AI-2/Lux-S Quorum Sensing of Lactobacillus plantarum SS-128 Prolongs the Shelf Life of Shrimp (Litopenaeus vannamei): From Myofibril Simulation to Practical Application

Li, Yuan; Liu, Taige; Meng, Xia; Qian, Yilin; Yan, Song Y.; Liu, Zunying

doi:10.3390/foods11152273

Cited by 4 publications

(1 citation statement)

References 49 publications

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“…The anti-QS activity of Bacillus strains was identified through the QQ activity of lactonase and the antagonistic effect of DKPs on QS receptors. Li et al (2022a) [ 81 ] showed that QS promoted the growth of Lb. plantarum ss-128, but they did not investigate its anti-QS activity against spoilage bacteria.…”

Section: Mechanism Of Qs Inhibitionmentioning

confidence: 99%

Anti-Quorum Sensing Activity of Probiotics: The Mechanism and Role in Food and Gut Health

2023

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Background: Quorum sensing (QS) is a cell-to-cell communication mechanism that occurs between inter- and intra-bacterial species and is regulated by signaling molecules called autoinducers (AIs). It has been suggested that probiotics can exert a QS inhibitory effect through their metabolites. Purpose: To provide an overview of (1) the anti-QS activity of probiotics and its mechanism against foodborne pathogenic and spoilage bacteria; (2) the potential role of the QS of probiotics in gut health; and (3) the impact of microencapsulation on QS. Results: Lactobacillus species have been extensively studied for their anti-QS activity and have been found to effectively disrupt QS in vitro. However, their effectiveness in a food matrix is yet to be determined as they interfere with the AI receptor or its synthesis. QS plays an important role in both the biofilm formation of probiotics and pathogenic bacteria. Moreover, in vitro and animal studies have shown that QS molecules can modulate cytokine responses and gut dysbiosis and maintain intestinal barrier function. In this scenario, microencapsulation was found to enhance AI activity. However, its impact on the anti-QS activity of probiotics and its underlying mechanism remains unclear. Conclusions: Probiotics are potential candidates to block QS activity in foodborne pathogenic and food spoilage bacteria. Microencapsulation increases QS efficacy. However, more research is still needed for the identification of the QS inhibitory metabolites from probiotics and for the elucidation of the anti-QS mechanism of probiotics (microcapsules and free cells) in food and the human gut.

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Section: Mechanism Of Qs Inhibitionmentioning

confidence: 99%

Anti-Quorum Sensing Activity of Probiotics: The Mechanism and Role in Food and Gut Health

2023

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The V‐Shaped Structuring Regulated via the LuxS‐Dependent Quorum‐Sensing Pathway Is Associated With Lactiplantibacillus plantarum Survivability in Acidic Environments

Venugopal,

Sionov,

Kroupitski

et al. 2024

Food Frontiers

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Tolerance to acidic environments is an important adaptive feature of the probiotic bacterium Lactiplantibacillus plantarum for surviving gastrointestinal transit or food processing. Here, we report a previously uncharacterized biological process that enables a highly coordinated adaptation of this bacterium to acidic stress. We provide mechanistic evidence for the role of the V‐shaped multicellular structuring, associated with incomplete daughter cell separation, in the survival of L. plantarum cells in acidic environments. We also show that this process facilitates structured biofilm formation through the LuxS‐dependent quorum‐sensing pathway associated with autoinducer‐2 (AI‐2) production. The LuxS knockout results in compromised V‐shaped structuring and poor biofilm formation under acidic conditions, whereas exogenous supplementation of 4,5‐dihydroxy‐pentanedione (DPD), the precursor of AI‐2, to the LuxS knockout strain, restores V‐shaped structuring and biofilm formation in an acidic environment. Furthermore, we show that LuxS‐dependent V‐shaped structuring provides an extraordinary protection mode for cellular survivability in extremely acidic conditions. Consequently, we propose that the self‐generated V‐shaped structuring, regulated by LuxS, is associated with protective and coordinated multicellular behavior during adaptation to acidic stress. It is believed that this study paves the way for developing a promising platform for preserving and delivering probiotic cells to mammalian hosts by utilizing persistent geometrical structuring.

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Erythromycin disrupts Acinetobacter baumannii biofilms through destruction of the quorum sensing system

Dong,

Sun,

Liu

et al. 2024

Medicine

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Background: This study was conducted to explore the effects of erythromycin on biofilms comprising Acinetobacter baumannii (A baumannii). Methods: To clarify the effect of erythromycin on the biofilms of A baumannii, we collected pure Ab strains isolated and identified from a variety of sample types extracted from patients in the microbiological laboratory of our hospital from April to August 2023, and divided them into an experimental group (treated with erythromycin) and a control group (without erythromycin). The morphology and quantity of A baumannii biofilm were observed at 24h, 48h, 72h, and 5d post-treatment, respectively, and the expression of quorum sensing (QS) system gene (abaI, abaR) mRNA was detected by fluorescence quantitative PCR. Results: The results showed that A baumanniis are prone to form multiple drug-resistant (MDR) bacteria, against which the most commonly used clinical antibiotics are ineffective. Overall, we found that the number of bacteria, the number of bacteria in the biofilm, and the number of biofilms formed gradually increased over time, with a statistical difference (P < .05). After the addition of erythromycin, significant improvements in biofilm formation were achieved, indicating that erythromycin can destroy A baumannii biofilms, inhibiting bacterial growth to a certain extent. The expression levels of abaI and abaR gradually increased over time, indicating that the role of the QS system became more apparent over time. Biofilm formation is related to the QS system of A baumanniis. After erythromycin treatment, abaI and abaR mRNA expression was downregulated in the experimental group. Conclusion: Erythromycin disrupts A baumannii biofilms by destroying the quorum sensing system.

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AI-2/Lux-S Quorum Sensing of Lactobacillus plantarum SS-128 Prolongs the Shelf Life of Shrimp (Litopenaeus vannamei): From Myofibril Simulation to Practical Application

Cited by 4 publications

References 49 publications

Anti-Quorum Sensing Activity of Probiotics: The Mechanism and Role in Food and Gut Health

Anti-Quorum Sensing Activity of Probiotics: The Mechanism and Role in Food and Gut Health

The V‐Shaped Structuring Regulated via the LuxS‐Dependent Quorum‐Sensing Pathway Is Associated With Lactiplantibacillus plantarum Survivability in Acidic Environments

Erythromycin disrupts Acinetobacter baumannii biofilms through destruction of the quorum sensing system

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