Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice

Tsuruta, Takeshi; Muhomah, Teresia Aluoch; Sonoyama, Kei; Nguyen, Qui D.; Takase, Yurika; Nishijima, Aoi; Himoto, Shiori; Katsumata, Emiko; Nishino, Norikazu

doi:10.1016/j.nutres.2021.06.009

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…When it was disrupted, the gut barrier allows bacterial toxins and other toxic substances enter the bloodstream (known as serum endotoxins). IgA is the most abundant antibody secreted into the gut and plays a crucial role in immune exclusion by neutralizing pathogenic toxins and preventing pathogen attachment and invasion across the gut barrier [ 23 ]. Therefore, we used gut occludin, serum endotoxin, and fecal IgA as indicators of damaged barrier function.…”

Section: Discussionmentioning

confidence: 99%

Effects of Amazake Produced with Different Aspergillus on Gut Barrier and Microbiota

Nakano,

Setoguchi,

Kawano

et al. 2023

Foods

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Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract. To explore the preventive effects of dietary foodson IBD, we evaluated the effects of the traditional Japanese fermented beverage “Amazake” on gut barrier function in this study. Black koji Amazake (BA) derived from Aspergillus luchuensis MEM-C strain and yellow koji Amazake (YA) derived from Aspergillus oryzae were made in this study, and their nutrients were analyzed. Mice with mild gut barrier dysfunction induced by Western diet were administered with 10% of each Amazake for two months. Mice gut microbiota were analyzed by 16S rRNA gene sequencing. BA contained a higher amount of isomaltooligosaccharides, citric acid, and ferulic acid than YA. The animal data revealed that BA significantly induced the expressions of antioxidant factors and enzymes such as NF-E2-related factor 2 (Nfr2), heme oxygenase 1 (HO1), and superoxide dismutase-2 (SOD-2). The gut barrier protein, occludin, and fecal immunoglobulin A (IgA) were also significantly enhanced by BA. Furthermore, the levels of serum endotoxin and hepatic monocyte chemotactic protein-1 (MCP-1) were decreased in both the BA and YA groups. In gut microbiota, Lachnospiraceae was increased by BA while Akkermansia muciniphilia was increased by YA. Black koji Amazake contained a higher amount of isomaltooligosaccharides, citric acid, and ferulic acid than yellow koji Amazake and contributed to protecting gut barrier function to reduce endotoxin intrusion and inflammation.

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Section: Discussionmentioning

confidence: 99%

Effects of Amazake Produced with Different Aspergillus on Gut Barrier and Microbiota

Nakano,

Setoguchi,

Kawano

et al. 2023

Foods

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“…[ 8 ] In addition, we recently demonstrated that HFD feeding reduces immunoglobulin A (IgA) coating of gut bacteria, and the reduction in IgA coating is related to HFD‐induced IR. [ 9 ] IgA is a major antibody isotype secreted into the gut and contributes to the maintenance of gut homeostasis. [ 10,11 ]…”

Section: Introductionmentioning

confidence: 99%

“…[8] In addition, we recently demonstrated that HFD feeding reduces immunoglobulin A (IgA) coating of gut bacteria, and the reduction in IgA coating is related to HFDinduced IR. [9] IgA is a major antibody isotype secreted into the gut and contributes to the maintenance of gut homeostasis. [10,11] Dietary fibers are defined as the edible parts of plants or analogous carbohydrates that are resistant to digestion and absorption in the human small intestine, with complete or partial fermentation in the colon.…”

Section: Introductionmentioning

confidence: 99%

Cyclic Nigerosylnigerose Attenuates High‐Fat Diet‐Induced Fat Deposition, Colonic Inflammation, and Abnormal Glucose Metabolism and Modifies Gut Immunoglobulin a Reactivity to Commensal Bacteria

Tsuruta

Sonoyama

Miyamoto

et al. 2023

Molecular Nutrition Food Res

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Scope High‐fat diet (HFD) intake induces gut dysbiosis, inflammation in the peripheral tissues, and a reduction in immunoglobulin A (IgA) coating of gut bacteria, which is related to HFD‐induced insulin resistance (IR). This study evaluates the effect of cyclic nigerosylnigerose (CNN), a dietary fiber that prevents gut inflammation and promotes IgA coating of gut bacteria, on the above‐mentioned HFD‐induced disorders. Methods and results Balb/c mice are fed an HFD and administered CNN for 20 weeks. CNN administration reduces mesenteric adipose tissue weight, colonic tumor necrosis factor α (TNFα) mRNA expression, and serum endotoxin levels and ameliorates HFD‐induced abnormal glucose metabolism. Additionally, CNN administration promotes gut bacteria‐specific IgA secretion and alters IgA reactivity to gut bacteria. The alterations of IgA reactivity to specific bacteria such as Erysipelatoclostridium, Escherichia, Faecalibaculum, Lachnospiraceae genera, and Stenotrophomonas are correlated with mesenteric adipose tissue weight, colonic TNFα mRNA expression, serum endotoxin levels, and a homeostasis model assessment for IR. Conclusion CNN‐induced alterations in IgA reactivity to gut bacteria may be related to the suppression of HFD‐induced fat deposition, colonic inflammation, endotoxemia, and IR. These observations indicate that dietary fiber that modulates IgA reactivity to gut bacteria may be useful in preventing HFD‐induced disorders.

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Unabsorbed Fecal Fat Content Correlates with a Reduction of Immunoglobulin a Coating of Gut Bacteria in High‐Lard Diet‐Fed Mice

Katsumata,

Tsuruta,

Sonoyama

et al. 2024

Molecular Nutrition Food Res

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ScopeImmunoglobulin A (IgA) selectively coats gut bacteria and contributes to regulatory functions in gastrointestinal inflammation and glucose metabolism. Excess intake of lard leads to decrease in the IgA coating of gut bacteria, although the underlying mechanisms remain unknown. This study validates how unabsorbed fat derived from a high‐lard diet in the gut affects the IgA coating of bacteria, as assessed in mouse models using three types of dietary fat (lard, medium‐, and long‐chain triglycerides [MLCTs], and medium‐chain triglycerides [MCTs]) exhibiting different digestibilities.Methods and resultsC57BL/6J mice are maintained on diets containing lard, MLCTs, or MCTs at 7% or 30% w/w for 10 weeks (n = 6 per group). The fecal fatty acid concentration is measured to quantify unabsorbed fat content. The ratio of IgA‐coated bacteria to total bacteria (IgA coating ratio) in the feces is measured by flow cytometry. Compared to lard‐fed mice, MLCT‐ and MCT‐fed mice exhibit lower fecal concentrations of palmitic acid, stearic acid, and oleic acid and higher IgA coating ratios at both 7% and 30% dietary fat, and these parameters exhibit significant negative correlations.ConclusionUnabsorbed fat content in the gut may result in attenuated IgA coating of bacteria in high‐lard diet‐fed mice.

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Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice

Cited by 3 publications

References 34 publications

Effects of Amazake Produced with Different Aspergillus on Gut Barrier and Microbiota

Effects of Amazake Produced with Different Aspergillus on Gut Barrier and Microbiota

Cyclic Nigerosylnigerose Attenuates High‐Fat Diet‐Induced Fat Deposition, Colonic Inflammation, and Abnormal Glucose Metabolism and Modifies Gut Immunoglobulin a Reactivity to Commensal Bacteria

Unabsorbed Fecal Fat Content Correlates with a Reduction of Immunoglobulin a Coating of Gut Bacteria in High‐Lard Diet‐Fed Mice

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