AIDS virus env Protein Expressed from a Recombinant Vaccinia Virus

Kiény, Marie-Paule; Rautmann, G; Schmitt, D; Dott, Karin; Wain‐Hobson, Simon; Alizon, Marc; Girard, Marc; Chamaret, S.; Laurent, A G; Montagnier, Luc; Lecocq, Jean‐Pierre

doi:10.1038/nbt0986-790

Cited by 94 publications

(65 citation statements)

References 47 publications

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“…The construction of recombinant VV encoding gpl60 (VV-gpl60) has been described elsewhere (4). VV-gpl6O-lL-2 was constructed by inserting the coding sequence for human lL-2 cDNA, excised from pcD-IL-2 (K. I. Arai.…”

Section: Construction Of Recomhinani Vaccinia Virus Encoding Gpl60 Anmentioning

confidence: 99%

Response of monkeys to vaccination with recombinant vaccinia virus which coexpress HIV gpl60 and human interleukin‐2

Ruby

Brinkman

Jones

et al. 1990

Immunology & Cell Biology

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Summary Immunization of two macaque monkeys with recombinant vaccinia viruses encoding the cnv gene of HlV-1 (VV-gpl60) resulled in demonstrable levels of gpl60, gpl20 and gp41-specific immunoglobulins in both animals. The virus used to immunize one ofthe monkeys additionally expressed Ihc human IL-2 gene, which encoded human [L-2 (VV-gpl60-IL-2). No toxic side-effects of vaecine-delivered IL-2 were observed. Despite marked attenuation of virulence by the coexpresscd lymphokine, the levels of vector-specific antibodies in both animals were similar. Some differences in the HI V-specifie reactivity patterns were detected. Serum reactivityof monkey #A56( VV-gp 160) was directed against gp41, whereas monkey #B58 (VV-gpl60-IL-2) showed a wider range of recognition, with higher antibody titres against the HIV lysate preparation. Furthermore, this study demonstrates the capacity to boost antibody responses to the vector and coexpresscd HIV antigens in primates which are already immune.

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Section: Construction Of Recomhinani Vaccinia Virus Encoding Gpl60 Anmentioning

confidence: 99%

Response of monkeys to vaccination with recombinant vaccinia virus which coexpress HIV gpl60 and human interleukin‐2

Ruby

Brinkman

Jones

et al. 1990

Immunology & Cell Biology

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“…All cell lines were cultivated at 37°C in a 5% CO 2 atmosphere and split twice a week. Stocks of recombinant VV (Copenhagen strain) were prepared on CV-1 cells as described by Kieny et al [23].…”

Section: Cells and Virusesmentioning

confidence: 99%

“…Recombinant VV were constructed and isolated as previously described [23,24]. Transfer plasmids, derived from pTG 186poly [23] were used to insert foreign sequences at the vaccinia TK locus.…”

Section: Construction Of Recombinant Vvmentioning

confidence: 99%

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Immunopotentiation of the antibody response against influenza HA with apoptotic bodies generated by rabies virus G-ERA protein-driven apoptosis

Mégret

Préhaud

Lafage

et al. 2005

Vaccine

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Apoptosis is considered to be a way of eliminating unwanted cells without causing major inflammation. Nevertheless, several lines of evidence show that apoptotic cell-derived antigens can be strong immunogens. The rabies virus glycoprotein G-ERA is an apoptotic molecule. We tested the ability of G-ERA to potentiate a B cell response against an exogenous antigen (influenza hemagglutinin, HA). We found that co-expression of G-ERA and HA in apoptotic bodies increased both the primary and memory HA-specific immune responses. The immunopotentiation of G-ERA is apoptosis-mediated but not necrosismediated. Our data indicate that G-ERA-mediated apoptosis might be useful to improve the immunogenicity of live vaccines.3

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“…24 For pTG1142, the cDNAs coding for the heavy and light chains were introduced into plasmid pTG186poly downstream of two copies of the 7.5K vaccinia promoter. 25 The expression blocks were placed in reverse orientations to avoid homologous recombination between the two copies of the promoter. For the construction of pTG2179, the cDNA coding for the heavy chain of mAb CO17-1A was cloned in phase upstream of the sequence coding for the transmembrane and intracytoplasmic domains of rabies glycoprotein.…”

Section: Vvsmentioning

confidence: 99%

Redirected cellular cytotoxicity by infection of effector cells with a recombinant vaccinia virus encoding a tumor-specific monoclonal antibody

et al. 2000

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Cytotoxicity is an important function of the immune system that results in the destruction of cellular targets by humoral and/or cellular mechanisms. We wanted to assess the possibility of targeting the lytic function of immune cells toward cancer cells, which express the gene coding for a known tumor antigen (Ag) (GA733-2/epithelial cell adhesion molecule), using a viral vector encoding a monoclonal antibody (mAb) specific for said tumor Ag (CO17-1A). To this end, we have constructed recombinant vaccinia viruses expressing the sequences corresponding to mAb CO17-1A, which recognizes a specific Ag (GA733-2) that is present on the surface of most gastrointestinal carcinomas. The recombinant vectors encoding either a secreted or membrane-anchored form of CO17-1A mAb were used to infect effector cells, which were subsequently assessed for their cytotoxic activity. The recombinant viruses were able to infect both granulocyte-macrophage colony-stimulating factor-activated human macrophages and Ag-stimulated murine cytotoxic T lymphocytes. Infected granulocyte-macrophage colony-stimulating factor-activated macrophages were found to be able to kill GA733-2-expressing tumor cells. Likewise, infected cytotoxic T lymphocytes, although conserving their original alloreactivity, gained the capability of killing GA733-2-expressing cancer cells. Cancer Gene Therapy (2000) 7, 615-623

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AIDS virus env Protein Expressed from a Recombinant Vaccinia Virus

Cited by 94 publications

References 47 publications

Response of monkeys to vaccination with recombinant vaccinia virus which coexpress HIV gpl60 and human interleukin‐2

Response of monkeys to vaccination with recombinant vaccinia virus which coexpress HIV gpl60 and human interleukin‐2

Immunopotentiation of the antibody response against influenza HA with apoptotic bodies generated by rabies virus G-ERA protein-driven apoptosis

Redirected cellular cytotoxicity by infection of effector cells with a recombinant vaccinia virus encoding a tumor-specific monoclonal antibody

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