AIEgen-Peptide Bioprobes for the Imaging of Organelles

Abstract

Organelles are important subsystems of cells. The damage and inactivation of organelles are closely related to the occurrence of diseases. Organelles’ functional activity can be observed by fluorescence molecular tools. Nowadays, a series of aggregation-induced emission (AIE) bioprobes with organelles-targeting ability have emerged, showing great potential in visualizing the interactions between probes and different organelles. Among them, AIE luminogen (AIEgen)-based peptide bioprobes have attracted more and more attention from researchers due to their good biocompatibility and photostability and abundant diversity. In this review, we summarize the progress of AIEgen-peptide bioprobes in targeting organelles, including the cell membrane, nucleus, mitochondria, lysosomes and endoplasmic reticulum, in recent years. The structural characteristics and biological applications of these bioprobes are discussed, and the development prospect of this field is forecasted. It is hoped that this review will provide guidance for the development of AIEgen-peptide bioprobes at the organelles level and provide a reference for related biomedical research.

Tyrosinase‐activated Nanocomposites for Double‐Modals Imaging Guided Photodynamic and Photothermal Synergistic Therapy

“…Mitochondria are the energy factories of cells. [55][56][57][58] IOBOH@BSA finally targeted mitochondria, which would be beneficial to target cancerous cells and improve the efficiency of tumor treatment.…”

A Review on Recent Advances in Peptide‐based Fluorescence Probes and their Potential Applications

“…[74] The peptide-based fluorescent probe usually possessed a large Stokes shift that can up to or above 170 nm. [21,73,[75][76][77] Wang et al designed a peptide fluorescence probe L based on the solid phase peptide synthesis method by combination of a tetrapeptide with Dansyl group. [78] According to the PET fluorescence quenching mechanism, the probe fluorescence was quenched and the fluorescence was restored after combined with Cd 2 + ion.…”

“…The small Stokes shifts of most dyes including fluorescein, rhodamine and cyanine usually within the range of 10–50 nm, which limited their application [74] . The peptide‐based fluorescent probe usually possessed a large Stokes shift that can up to or above 170 nm [21,73,75–77] . Wang et al .…”

Aggregation-Induced Enhanced Emission of Tetraphenylethene-phenylalanine Hybrids: Synthesis and Characterization

“…Among AIEgens, tetraphenylethene (TPE, Figure , 2 ) has been widely used as a building block for the construction of a number of fluorescent sensors and probes. − The AIE property of TPE is due to the restriction of intramolecular rotation and intramolecular π–π stacking interactions that occur when it is in the aggregated state. In the medical field, TPE has been conjugated with peptides to develop specific bioprobes for real-time monitoring and understanding of complex processes at the molecular level. ,− First, the Liu group synthesized a fluorescent light-up sensor by conjugating a TPE moiety with a caspase-specific hydrophilic peptide (DEVDK) via a click reaction.…”