“…A multi-institutional team led by Louise Laurent (University of California San Diego, contact PI) will focus on using immunoaffinity separation (IMS) for isolation of exRNA carrier subclasses (CSs). This transdisciplinary team, with expertise in exRNA and lipoprotein biology ( Li et al., 2018b ), exRNA biomarker discovery ( Chen-Plotkin et al., 2018 ; Srinivasan et al., 2020 ; Xiao et al., 2017 ), exRNA sequencing ( Rodosthenous et al., 2020 ; Srinivasan et al., 2019 ), low-input proteomics ( Tsai et al., 2021 ), lipidomics ( Wang et al., 2021b ), and flow cytometry ( Görgens and Nolan, 2020 ; Nolan and Duggan, 2018 ; Welsh et al., 2020 ), will work together to develop and apply a rigorous, reproducible, efficient, scalable, and cost-effective workflow for preparative isolation of subtypes of EVs, RNPs, and LPPs for downstream-omics analysis. This team’s initial approach will be to use antibodies conjugated to magnetic nanoparticles, but they will also explore the use of multiplex bead-based flow sorting for even more efficient separation of CSs.…”