1995
DOI: 10.1128/jb.177.23.6946-6951.1995
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AinS and a new family of autoinducer synthesis proteins

Abstract: In Vibrio fischeri, the autoinducer N-3-oxohexanoyl-L-homoserine lactone (AI-1) governs the cell densitydependent induction of the luminescence operon via the LuxR transcriptional activator. The synthesis of AI-1 from bacterial metabolic intermediates is dependent on luxI. Recently, we found a second V. fischeri autoinducer molecule, N-octanoyl-L-homoserine lactone (AI-2), that in E. coli also activates the luminescence operon via LuxR. A locus independent of luxI was identified as being required for AI-2 synt… Show more

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Cited by 170 publications
(162 citation statements)
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“…These results have led us to suspect that the AI-2s are not acyl-homoserine lactones. The luxS genes identified here bear no homology to other genes known to be involved in production of HSL autoinducers [luxI-like (1), luxLM-ainS-like (11,21)], further indicating that the AI-2 class of autoinducers is novel.…”
Section: Discussionmentioning
confidence: 69%
“…These results have led us to suspect that the AI-2s are not acyl-homoserine lactones. The luxS genes identified here bear no homology to other genes known to be involved in production of HSL autoinducers [luxI-like (1), luxLM-ainS-like (11,21)], further indicating that the AI-2 class of autoinducers is novel.…”
Section: Discussionmentioning
confidence: 69%
“…We plan to construct a new gene library from the expI mutant and screen it using E. coli/pSB401 acyl-HSL sensor strain with a longer exposure time. This second genetic locus could contain a second luxI homologue, or it may be related to the ainS locus of V. fischeri or to the luxLM locus of V. harveyi, both of which direct acyl-HSL synthesis but display no homology with luxI (Bassler et al, 1993;Gilson et al, 1995). The signal transducers AinR and LuxN, associated with AinS and LuxLM respectively, appear to autophosphorylate in the presence of their cognate acyl-HSL and induce a phosphoryl-transfer cascade that exerts control over bioluminescence (Bassler et al, 1993;Gilson et al, 1995).…”
Section: Discussionmentioning
confidence: 99%
“…Generally speaking, AHLs are biosynthesized by members of the LuxI family of AHL synthases using the substrates S-adenosylmethionine (SAM) and an acylated acyl carrier protein (acyl-ACP) (27). Exceptions to this are LuxM and AinS of Vibrio harveyi and Vibrio fischeri, respectively, which catalyze the same reaction but do not share homology with LuxI-type proteins (28). In general, each AHL synthase predominantly makes a single type of AHL, although some synthases also produce additional AHLs in smaller amounts.…”
Section: Ahl-based Quorum Sensingmentioning
confidence: 99%
“…AHL signals are produced by acyl-HSL synthases belonging to the LuxI or AinS family and are formed from the substrates SAM and acyl-ACP (27,28). As such, inhibition of AHL production could theoretically be achieved through suppression of SAM biosynthesis, interference with generation of acyl-ACPs, or inactivation of the synthase enzyme.…”
Section: Inhibition Of Ahl Productionmentioning
confidence: 99%