AINTEGUMENTA negatively regulates age-dependent leaf senescence downstream of AUXIN RESPONSE FACTOR 2 in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

Xu, Qian; Wang, Zhenyang; Zhuoma, Qiangjiu

doi:10.5511/plantbiotechnology.16.0222a

Cited by 17 publications

(12 citation statements)

References 40 publications

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“…Leaf senescence constitutes the final phase of leaf development and is a highly complex but genetically programmed process involving the expression of many senescence-associated genes ( SAGs ) 55 , 56 . Our study shows that, compared with the wild type, leaf senescence in the 35S-BrANT-1 transgenic line was delayed, which is consistent with that in Arabidopsis over-expressing the AtANT gene 57 . Additionally, a number of SAGs such as AtNAC2 (AT5G39610) 58 , AtSAUR36 (AT2G45210) 45 , and AtSAG13 (AT2G29350) 59 were prominently down-regulated in the 35S-BrANT-1 line.…”

Section: Discussionsupporting

confidence: 87%

Ectopic expression of a Brassica rapa AINTEGUMENTA gene (BrANT-1) increases organ size and stomatal density in Arabidopsis

Ding

Cui

et al. 2018

Sci Rep

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The AINTEGUMENTA-like (AIL) family plays a central role in regulating the growth and development of organs in many plants. However, little is known about the characteristics and functions of the AIL family in Chinese cabbage (Brassica rapa L. ssp. pekinensis). In this study, a genome-wide analysis was performed to identify the members of the AIL family in Chinese cabbage. We identified three ANT genes and six ANT-like genes of Chinese cabbage, most of which were differentially expressed in different organs or tissues. Furthermore, compared with the wild-type line, the size of different organs in the 35S-BrANT-1 line was significantly increased by promoting cell proliferation. Meanwhile, over-expression of BrANT-1 also increases the stomatal number and delays the leaf senescence. Transcriptome analyses revealed that a set of cell proliferation and stoma development genes were up-regulated, while the senescence-associated genes were down-regulated, suggesting these genes may be involved in BrANT-1 regulated processes for controlling organ size, stomatal density and leaf senescence. In summary, this study offers important insights into the characteristics and functions of the ANT genes in Chinese cabbage, and provides a promising strategy to improve yield or head size in Chinese cabbage breeding programs.

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Section: Discussionsupporting

confidence: 87%

Ectopic expression of a Brassica rapa AINTEGUMENTA gene (BrANT-1) increases organ size and stomatal density in Arabidopsis

Ding

Cui

et al. 2018

Sci Rep

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“…As previously reported, an3‐4 plants had smaller leaves and a reduced cell number compared with Col plants (Fig. d,e; Feng et al ., ). ore15‐2 an3‐4 plants had a much smaller leaf size and cell number, and ore15‐1D an3‐4 plants showed intermediate phenotypes compared with the two parental lines (Figs d,e, S8c,d), indicating a composite regulatory interaction between ORE15 and GIF1/AN3 in the regulation of leaf growth.…”

Section: Resultsmentioning

confidence: 97%

“…In addition, a mutant of the GIF1 gene, also known as ANGUSTIFOLIA3 ( AN3 ), exhibits similar phenotypes shown in the grf3 and grf5 mutants (Horiguchi et al ., ; Debernardi et al ., ). AINTEGUMENTA (ANT), another important leaf growth regulator through cell proliferation, has been implicated in the regulation of age‐dependent leaf senescence by acting downstream of ARF2 in Arabidopsis (Mizukami & Fischer, ; Feng et al ., ).…”

Section: Introductionmentioning

confidence: 97%

ORESARA15, a PLATZ transcription factor, mediates leaf growth and senescence in Arabidopsis

Kim

Jun

et al. 2018

New Phytologist

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Plant leaves undergo a series of developmental changes from leaf primordium initiation through growth and maturation to senescence throughout their life span. Although the mechanisms underlying leaf senescence have been intensively elucidated, our knowledge of the interrelationship between early leaf development and senescence is still fragmentary. We isolated the oresara15-1Dominant (ore15-1D) mutant, which had an extended leaf longevity and an enlarged leaf size, from activation-tagged lines of Arabidopsis. Plasmid rescue identified that ORE15 encodes a PLANT A/T-RICH SEQUENCE- AND ZINC-BINDING PROTEIN family transcription factor. Phenotypes of ore15-1D and ore15-2, a loss-of-function mutant, were evaluated through physiological and anatomical analyses. Microarray, quantitative reverse transcription polymerase chain reaction, and chromatin immunoprecipitation as well as genetic analysis were employed to reveal the molecular mechanism of ORE15 in the regulation of leaf growth and senescence. ORE15 enhanced leaf growth by promoting the rate and duration of cell proliferation in the earlier stage and suppressed leaf senescence in the later stage by modulating the GROWTH-REGULATING FACTOR (GRF)/GRF-INTERACTING FACTOR regulatory pathway. Our study highlighted a molecular conjunction through ORE15 between growth and senescence, which are two temporally separate developmental processes during leaf life span.

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“…Taken all together, we postulate that genes related to auxin play either positive or negative roles in regulating corolla senescence. A recent study has illustrated that AINTEGUMENTA ( ANT ), a member of the AP2/ERF TF family functioning downstream of ARF2 in Arabidopsis , negatively regulated leaf senescence 48 . The underlying mechanisms how ARFs regulate onset of corolla senescence require further studies in the future.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling reveals regulatory mechanisms underlying corolla senescence in petunia

et al. 2018

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The genetic regulatory mechanisms that govern natural corolla senescence in petunia are not well understood. To identify key genes and pathways that regulate the process, we performed a transcriptome analysis in petunia corolla at four developmental stages, including corolla fully opening without anther dehiscence (D0), corolla expansion, 2 days after anthesis (D2), corolla with initial signs of senescence (D4), and wilting corolla (D7). We identified large numbers of differentially expressed genes (DEGs), ranging from 4626 between the transition from D0 and D2, 1116 between D2 and D4, a transition to the onset of flower senescence, and 327 between D4 and D7, a developmental stage representing flower senescence. KEGG analysis showed that the auxin- and ethylene-related hormone biosynthesis and signaling transduction pathways were significantly activated during the flower development and highly upregulated at onset of flower senescence. Ethylene emission was detected at the D2 to D4 transition, followed by a large eruption at the D4 to D7 transition. Furthermore, large numbers of transcription factors (TFs) were activated over the course of senescence. Functional analysis by virus-induced gene silencing (VIGS) experiments demonstrated that inhibition of the expression of TFs, such as ethylene-related ERF, auxin-related ARF, bHLH, HB, and MADS-box, significantly extended or shortened flower longevity. Our data suggest that hormonal interaction between auxin and ethylene may play critical regulatory roles in the onset of natural corolla senescence in petunia.

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AINTEGUMENTA negatively regulates age-dependent leaf senescence downstream of AUXIN RESPONSE FACTOR 2 in <i>Arabidopsis thaliana</i>

Cited by 17 publications

References 40 publications

Ectopic expression of a Brassica rapa AINTEGUMENTA gene (BrANT-1) increases organ size and stomatal density in Arabidopsis

Ectopic expression of a Brassica rapa AINTEGUMENTA gene (BrANT-1) increases organ size and stomatal density in Arabidopsis

ORESARA15, a PLATZ transcription factor, mediates leaf growth and senescence in Arabidopsis

Transcriptome profiling reveals regulatory mechanisms underlying corolla senescence in petunia

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