Air Particulate Matter Induces Skin Barrier Dysfunction and Water Transport Alteration on a Reconstructed Human Epidermis Model

Hieda, Daniele Seo; Carvalho, Larissa Anastácio da Costa; Mello, Barbara Vaz de; Oliveira, Érica Aparecida de; Assis, Sílvia Romano de; Wu, Joanna X.; Du‐Thumm, Laurence; Silva, Claudia Larissa Viana da; Roubicek, Deborah A.; Maria‐Engler, Silvya Stuchi; Barros, Sílvia Berlanga de Moraes

doi:10.1016/j.jid.2020.03.971

Cited by 29 publications

(30 citation statements)

References 44 publications

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“…In this study, we demonstrate significant inhibition of FLG expression in keratinocyte cultures, organotypic skin cultures, and the skin of the animals exposed in vivo to PM2.5. This observation is consistent with previous reports (27,29). However, this is the first study that examined the effects of PM2.5 on skin both in vitro and in vivo.…”

Section: Discussionsupporting

confidence: 93%

“…In contrast, earlier studies examined the effects of PM in HaCat tumor keratinocyte cell line and human keratinocytes, using doses that far exceed those in the environment. We found that these PM2.5 doses were cytotoxic to primary keratinocytes in the current study (27)(28)(29).…”

Section: Discussionsupporting

confidence: 54%

“…Most published papers related to PM2.5 have focused on cardiovascular and airway diseases, with very few studies examining the effects of PM2.5 in the skin. To date, limited and conflicting data regarding the effects of PM on skin and skin barrier proteins have been reported (27)(28)(29). Given the importance of potential detrimental effects of PM2.5 skin exposure, this study was done to evaluate the effects of PM2.5 on skin barrier function using in vitro and in vivo models.…”

Section: Introductionmentioning

confidence: 99%

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Particulate matter causes skin barrier dysfunction

et al. 2021

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The molecular mechanisms that underlie the detrimental effects of particulate matter (PM) on skin barrier function are poorly understood. In this study, the effects of PM2.5 on filaggrin (FLG) and skin barrier function were investigated in vitro and in vivo. The levels of FLG degradation products including pyrrolidone carboxylic acid, urocanic acid (UCA), and cis/trans UCA were significantly decreased in skin tape stripping samples of study subjects when they moved from Denver, an area with low PM2.5, to Seoul, an area with high PM2.5 count. Experimentally, PM2.5 collected in Seoul inhibited FLG, loricrin, keratin-1, desmocollin-1, and corneodesmosin, but did not modulate involucrin or claudin-1 in keratinocyte cultures. Moreover, FLG protein expression was inhibited in human skin equivalents and murine skin treated with PM2.5. We demonstrate that this process was mediated by PM2.5-induced TNF-alpha and was aryl hydrocarbon receptor-dependent. PM2.5 exposure compromised skin barrier function, resulting in increased transepidermal water loss and enhanced the penetration of FITC-dextran in organotypic and mouse skin. PM2.5-induced TNF-alpha causes FLG deficiency in the skin and subsequently induces skin barrier dysfunction. Compromised skin barrier due to PM2.5 exposure may contribute to the development and the exacerbation of allergic diseases such as AD.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Particulate matter causes skin barrier dysfunction

et al. 2021

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“…The cells from RHEs tissues were lysed, according to Hieda et al [41]. Protein concentrations were determined with the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA).…”

Section: Protein Expression By Western Blottingmentioning

confidence: 99%

“…Total protein (40 µg) was subjected to electrophoresis in 8%, 12%, and 15% polyacrylamide gels under reducing conditions and transferred to polyvinylidene fluoride membranes (Hybond, Amersham Pharmacia Biotech, Piscataway, NJ, USA). The membranes were blocked in 3% BSA diluted in TBS-Tween 20 [41] for 1 h at room temperature and probed with the following antibodies: against cytokeratin 14 (Abcam-ab7800 1:1000), against cytokeratin 10 (Abcam ab76318 1:1000) and against involucrin (Abcam-ab27495 1:1000), and against β-actin (Abcam ab8227 1:2000) overnight at 4 • C. After washing, the membranes were incubated for 1 h at room temperature with the secondary antibody. Protein bands were detected by an enhanced chemiluminescence system (ECL, Amersham Pharmacia Biotech, Piscataway, NJ, USA).…”

Section: Protein Expression By Western Blottingmentioning

confidence: 99%

Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing

et al. 2020

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The development of products for topical applications requires analyses of their skin effects before they are destined for the market. At present, the ban on animal use in several tests makes the search for in vitro models (such as artificial skin) necessary to characterize the risks involved. In this work, tissue engineering concepts were used to manufacture collagen-free three-dimensional scaffolds for cell growth and proliferation. Two different human skin models—reconstructed human epidermis and full-thickness skin—were developed from electrospun scaffolds using synthetic polymers such as polyethylene terephthalate, polybutylene terephthalate, and nylon 6/6. After the construction of these models, their histology was analyzed by H&E staining and immunohistochemistry. The results revealed a reconstructed epidermal tissue, duly stratified, obtained from the nylon scaffold. In this model, the presence of proteins involved in the epidermis stratification process (cytokeratin 14, cytokeratin 10, involucrin, and loricrin) was confirmed by immunohistochemistry and Western blot analysis. The nylon reconstructed human epidermis model’s applicability was evaluated as a platform to perform irritation and corrosion tests. Our results demonstrated that this model is a promising platform to assess the potential of dermal irritation/corrosion of chemical products.

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Assessing the impact of airborne particulate pollution on human skin utilizing a novel human skin equivalent containing MUTZ‐3‐derived Langerhans cells

Simpson,

DiColandrea,

Przyborski

2024

Bioengineering & Transla Med

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Air pollution is an exogenous stressor known to have a detrimental impact on skin health through the induction of inflammation; however, the direct effect of topical pollution exposure is still being elucidated. Human skin equivalents (HSE) aim to reproduce in vitro the structure and function of the native skin tissue. However, HSEs typically lack skin‐resident immune cells, which could play a key role in the inflammatory response induced by pollution exposure. We outline the development of a HSE‐containing MUTZ‐3‐derived Langerhans cells (MUTZ‐3‐LCs), which show dendritic morphology and Langerhans cell marker expression. We demonstrated that HSE‐containing MUTZ‐3‐LC have lower basal levels of proinflammatory cytokines, but topical stimulation with allergens and irritant compounds induced a greater inflammatory response in these models compared to HSE without immune cells. To study the effect of pollution, we created a technique to apply diesel particulate matter (DPM) to HSEs. Though our microscopic analysis demonstrated that DPM does not penetrate the stratum corneum, we showed that DPM did induce production of proinflammatory cytokines, but notably only in HSEs containing MUTZ‐3‐LCs. These data suggest that topical exposure to air pollution can induce cutaneous inflammation and that skin‐resident immune cells contribute to this response. This highlights the significance of immune‐competent HSEs to the study of exogenous stressors in vitro.

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Air Particulate Matter Induces Skin Barrier Dysfunction and Water Transport Alteration on a Reconstructed Human Epidermis Model

Cited by 29 publications

References 44 publications

Particulate matter causes skin barrier dysfunction

Particulate matter causes skin barrier dysfunction

Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing

Assessing the impact of airborne particulate pollution on human skin utilizing a novel human skin equivalent containing MUTZ‐3‐derived Langerhans cells

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