Air pollution in Sarajevo, Bosnia and Herzegovina, assessed by plant comet assay

Hasanović, Mujo; Ćetković, Tamara; Pourrut, Bertrand; Klačar, Lejla Čaluk; Omanovic, Maida Hadzic; Durmić-Pašić, Adaleta; Haverić, Sanin; Haverić, Anja

doi:10.1093/mutage/geac022

Cited by 10 publications

(4 citation statements)

References 48 publications

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“…However, human biomonitoring remains the best approach for evaluating the effects of complex mixtures, providing data for further in vitro and in silico studies, and scientifically based risk assessment [ 72 , 73 , 74 , 75 ]. Recent studies suggest that sentinel organisms such as butterflies and plants, sensitive to air pollutants, could serve as early warning signs for human biomonitoring studies [ 76 , 77 , 78 , 79 ]. Future prospective studies should include biomarkers of air pollution exposure, human exposure conditions, and assessment of biomarkers of oxidative stress and inflammation, as these events might occur earlier in the exposure–effect scenario, forming a basis for DNA damage induction.…”

Section: Discussionmentioning

confidence: 99%

Air Pollution and Primary DNA Damage among Zagreb (Croatia) Residents: A Cross-Sectional Study

Gerić,

Pehnec,

Matković

et al. 2024

JoX

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More than eight million premature deaths annually can be attributed to air pollution, with 99% of the world’s population residing in areas below recommended air quality standards. Hence, the present study aimed to examine the association between primary DNA damage and air pollution data among 123 participants enrolled between 2011 and 2015 in Zagreb, Croatia. While most measured air pollutants adhered to regulatory limits, benzo[a]pyrene concentrations bound to PM10 exceeded them. Factorial analysis narrowed down air pollution data to four exposure factors (particulate matter, two metal factors, and other pollutants). Despite the absence of significant positive associations between modeled air pollution exposure factors and comet assay descriptors (tail length, tail intensity, tail moment, and highly damaged nuclei), the critical health implications of air pollution warrant further investigations, particularly with biomarkers of exposure and different biomarkers of effect in populations facing air pollution exposure.

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Section: Discussionmentioning

confidence: 99%

Air Pollution and Primary DNA Damage among Zagreb (Croatia) Residents: A Cross-Sectional Study

Gerić,

Pehnec,

Matković

et al. 2024

JoX

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“…The leaves of deciduous plants can be used as bioindicators of the DNA damage caused by various genotoxic factors such as radiation, chemicals, allelochemicals, heavy metals, nanoparticles, or complex contaminants originating from the atmosphere and soil. Ligustrum vulgare (common privet) has been used as a model to monitor the air pollution using Comet assays: analyses of leaves harvested at three locations with different levels of air pollution in Bosnia and Herzegovina, including two urban and one rural location, showed that DNA damage measured as tail intensity depended on the sampling period, leaf position, and growth stage, and showed clear differences between urban and rural locations [147]. Furthermore, this model proved sensitive to seasonal variations in air pollution levels since DNA damage in L. vulgare leaves was proportional to the average concentration of particulate matter of a size ranging from 0.001 to 2.5 µm in diameter (PM 2.5 ) and depended on indoor vs. outdoor conditions [147].…”

Section: Comet Assays In Plant Ecotoxicology and Biological Monitoringmentioning

confidence: 99%

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Tyutereva,

Strizhenok,

Kiseleva

et al. 2024

Horticulturae

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Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications; however, they are still relatively rarely used in physiological studies. In this review, we provide an overview of the basic principles and of useful variations of the protocols of Comet assays, as well as of their use in plant studies, in order to encourage plant physiologists to include this tool in the analysis of plant stress responses.

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“…Temperature inversion, as a natural phenomenon, occurs when temperatures at higher altitudes are higher than temperatures at lower altitudes, causing pollutants to be stuck and leading to higher pollutant concentrations in the air. The problem of air pollution is one of the major problems in the city of Sarajevo, where the citizens often face excessive levels of air pollutants [1,2]. The problem of air pollution is particularly present in the winter period and the heating season in the months of November, December, January, February and March.…”

Section: Introductionmentioning

confidence: 99%

Principal Component Regression Modeling and Analysis of PM10 and Meteorological Parameters in Sarajevo with and without Temperature Inversion

et al. 2023

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The specific geographic location of Sarajevo, which is located in a valley surrounded by mountains, provides the opportunity to analyze the relation between the concentration of PM10 and meteorological parameters with and without temperature inversion. The main aim of this paper was to develop forecasting models of the hourly average of PM10 values in the Sarajevo urban area based on meteorological parameters measured in Sarajevo and on the Bjelasnica mountain with and without temperature inversion by using principal component regression (PCR). Also, this research explored and analyzed the differences in the values of the meteorological parameters and PM10 in Sarajevo with and without temperature inversion, and the difference in temperatures between Sarajevo and Bjelasnica with temperature inversion using statistical hypothesis testing with a total of 240 hypothesis tests performed. The measurements of meteorological parameters were taken from 2020 to 2022 for both Sarajevo (630 m) and the Bjelasnica mountain (2067 m), which allowed for the identification of time periods with and without temperature inversion, while measurements of PM10 were taken only in Sarajevo. Data were collected during the heating season (November, December, January, February and March). Since analyses have shown that only January and November had time periods with and without temperature inversion during each hour of the day, a total of seven cases were identified: two cases with and five cases without temperature inversion. For each case, three PCR models were developed using all principal components, backward elimination and eigenvalue principal component elimination criteria (λ<1). A total of 21 models were developed. The performance of the models were evaluated based on the coefficient of determination R2 and the standard error SE. The backward elimination models were shown to have high performances with the highest value of R2= 97.19 and the lowest value of SE=1.32. The study showed that some principal components with eigenvalues λ<1 were significantly related to the independent variable PM10 and thus were retained in the PCR models. In the study, it was shown that backward elimination PCR was an adequate tool to develop PM10 forecasting models with high performances and that it could be useful for authorities for early warnings or other action to protect citizens from very harmful pollution. Hypothesis tests showed different relations of meteorological parameters and PM10 with and without temperature inversion.

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Air pollution in Sarajevo, Bosnia and Herzegovina, assessed by plant comet assay

Cited by 10 publications

References 48 publications

Air Pollution and Primary DNA Damage among Zagreb (Croatia) Residents: A Cross-Sectional Study

Air Pollution and Primary DNA Damage among Zagreb (Croatia) Residents: A Cross-Sectional Study

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Principal Component Regression Modeling and Analysis of PM10 and Meteorological Parameters in Sarajevo with and without Temperature Inversion

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