Airborne pollen in Kiev (Ukraine): gravimetric sampling

Savitsky, Vladimir D.; Bezusko, L.G.; Butich, Natalia G.; Tsymbaliuk, Zoya M.; Savitska, Olena V.; Bezus'ko, Timur V.

doi:10.1007/bf02248153

Cited by 4 publications

(1 citation statement)

References 2 publications

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“…The morphological characteristics of pollen grains are often used in taxonomy as additional diagnostic features (Nowicke 1975; Mosyakin and Tsymbalyuk 2015, 2017, Albach et al 2021; Tsymbalyuk et al 2021a, 2021b, 2022a, 2023; El Ghazali 2022; Kurşat et al 2022; Bahadur et al 2023), as well as for spore–pollen analysis in paleopalynology (Monoszon 1973; Bezusko et al 2003, 2006, 2019; Gerasimenko et al 2022; Usma et al 2022; Ahmad et al 2023). Many authors have reported representatives of the Chenopodiaceae/Amaranthaceae group as allergenic pollen producers (Savitsky et al 1996; Ianovici 2008; Rodríguez de la Cruz et al 2012; Angelini et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Taxonomic significance of pollen morphology of selected taxa of Bassia , Sedobassia , Spirobassia and Neokochia (Chenopodiaceae/Amaranthaceae sensu APG IV)

2023

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Based on molecular phylogenetic and morphological studies, the new genera Sedobassia Freitag & G. Kadereit and Spirobassia Freitag & G. Kadereit were described in the tribe Camphorosmeae (Chenopodiaceae/Amaranthaceae sensu APG IV). Palynomorphological peculiarities are often used in taxonomy as additional diagnostic features. Pollen grains from 26 herbarium specimens belonging to 13 taxa of Bassia, Sedobassia, Spirobassia and Neokochia genera in Eurasia and North America were acetolyzed and studied using both light microscopy and scanning electron microscopy. Dry pollen grains were also studied. The structure of the columellae of pollen grains was analyzed for the first time in the current study. The aim of this research was to provide data on their pollen characteristics and to evaluate the taxonomic value of these data for generic and species-specific identification. The pollen grains are pantoporate, spheroidal in shape, circular in outline, slightly undulate, undulate or straight on the edges; and medium- or small-sized. Pores are circular, with distinct or indistinct margins. Exine sculpture is nanoechinate; tectum psilate or psilate-perforate. Pore membranes with nanoechini. Taxonomic relevance of the most important characters of pollen grains is discussed (pollen diameter, pore number, pore diameter, distance between pores and between pore centers, pollen diameter(C)/distance between pore centers(D) value, nanoechini density, presence/absence of perforations, structure of columellae, number of nanoechini on pore membranes). Pollen morphology proved to be an important additional source of information for species-specific identification. This study provides new data and analyzes the morphological features of pollen grains of Bassia (in the currently accepted circumscription), palynomorphological similarities, and differences between taxa of the genera Bassia, Sedobassia, Spirobassia, and Neokochia.

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Section: Introductionmentioning

confidence: 99%

Taxonomic significance of pollen morphology of selected taxa of Bassia , Sedobassia , Spirobassia and Neokochia (Chenopodiaceae/Amaranthaceae sensu APG IV)

2023

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Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Ražná

Bežo

Nikolaieva

et al. 2014

Journal of Environmental Science and Health, Part B

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Corylus avellana is the source of inhalant allergies induced by hazel pollen as well as food allergies induced after ingestion of hazelnuts. In this study, real-time PCR approach was used to analyse expression of hazel pollen allergens on the molecular level. Relative quantity of hazelnut allergens Corylus avellana, L. CorA and Corylus avellana, L. pollen profiling in samples from different Ukraine areas were determining and comparing. Differences among the levels of both analysed allergen transcripts were found for hazel CorA and profillin. In both cases, the expression within the urbanized growth conditions was higher when compared to the sample from village area. The average expression for CorA was 0.84 times higher than for profilin and the results are very variable depending on the place of growth. Expression levels here were within the range of 2.957 up to the 52.936. Profilin expression was the highest in the sample from the polluted place of growth-cement plant area with the value of 52 times higher when compared to the sample from the village area. In this study, comparison of expression levels of hazel CorA and profiling pollen allergens was performed for the first time. Real-time PCR assay developed in this study proved the sensitivity for detection of the changes of the hazel pollen allergens expression levels and could benefit labs by fast and reproducible detection method of these allergens.

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Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine

Žiarovská

Labajová

Ražná

et al. 2013

Journal of Environmental Science and Health, Part A

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The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes-cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Betv1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.

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Airborne pollen in Kiev (Ukraine): gravimetric sampling

Cited by 4 publications

References 2 publications

Taxonomic significance of pollen morphology of selected taxa of Bassia , Sedobassia , Spirobassia and Neokochia (Chenopodiaceae/Amaranthaceae sensu APG IV)

Taxonomic significance of pollen morphology of selected taxa of Bassia , Sedobassia , Spirobassia and Neokochia (Chenopodiaceae/Amaranthaceae sensu APG IV)

Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine

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