A dual-wavelength lidar fluorosensor system for fast diagnosis of chromophoric dissolved matter (CDOM) in water in the Pearl River estuary was discussed. The laser-induced fluorescence (LIF) system used two lasers as excitation sources with wavelength at 355nm and 532 nm, and a hyperspectral CCD spectrometer was used to record the fluorescence signal. The overlapping fluorescence spectra of water Raman scattering and CDOM were separated with fitting biGaussian of the least squares method. High correlation was observed between concentration of CDOM and fluorescence normalized to water Raman scattering. The in situ results demonstrated rapid characterization of dissolved organic matter can be done by the LIF technique.