Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice

Serré, Jef; Mathyssen, Carolien; Ajimé, Tom Tanjeko; Korf, Hannelie; Maes, Karen; Heulens, Nele; Gysemans, Conny; Mathieu, Chantal; Vanaudenaerde, Bart M.; Janssens, Wim; Gayan‐Ramirez, Ghislaine

doi:10.1016/j.jsbmb.2018.10.021

Cited by 15 publications

(34 citation statements)

References 50 publications

(86 reference statements)

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“…Mice were acclimatized during one week to CS by gradually increasing the number of cigarettes per day while the control mice were exposed to room air in soft restrains for the same duration ( Figure S1). The mice were nose-only exposed (InExpose System, SCIREQ, Montreal, Canada) to either six 3R4F research cigarettes (Kentucky Tobacco Research and Development Center, University of Kentucky) or room air (Con) in soft restrains twice daily, 5 days a week ( Figure S1), as previously described 25 . Total particle density was measured daily (Microdust, Casella CEL, Bedford, UK), and carbon monoxide (CO) levels in CS 3 times per week (EasyLog EL-USB-CO, Lascar electronics, UK), while body mass and food intake were measured weekly.…”

Section: Animals and Study Designmentioning

confidence: 99%

Two Weeks of Smoking Cessation Reverse Cigarette Smoke-Induced Skeletal Muscle Atrophy and Mitochondrial Dysfunction in Mice

Ajimé

Serré

Wüst

et al. 2020

Nicotine &Amp; Tobacco Research

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Introduction Apart from its adverse effects on the respiratory system, cigarette smoking also induces skeletal muscle atrophy and dysfunction. Whether short-term smoking cessation can restore muscle mass and function is unknown. We, therefore, studied the impact of 1- and 2-week smoking cessation on skeletal muscles in a mouse model. Methods Male mice were divided into four groups: Air-exposed (14 weeks); cigarette smoke (CS)-exposed (14 weeks); CS-exposed (13 weeks) followed by 1-week cessation; CS-exposed (12 weeks) followed by 2 weeks cessation to examine exercise capacity, physical activity levels, body composition, muscle function, capillarization, mitochondrial function and protein expression in the soleus, plantaris, and diaphragm muscles. Results CS-induced loss of body and muscle mass was significantly improved within 1 week of cessation due to increased lean and fat mass. Mitochondrial respiration and protein levels of the respiratory complexes in the soleus were lower in CS-exposed mice, but similar to control values after 2 weeks of cessation. Exposing isolated soleus muscles to CS extracts reduced mitochondrial respiration that was reversed after removing the extract. While physical activity was reduced in all groups, exercise capacity, limb muscle force, fatigue resistance, fiber size and capillarization, and diaphragm cytoplasmic HIF-1α were unaltered by CS-exposure. However, CS-induced diaphragm atrophy and increased capillary density were not seen after 2 weeks of smoking cessation. Conclusion In male mice, 2 weeks of smoking cessation reversed smoking-induced mitochondrial dysfunction, limb muscle mass loss, and diaphragm muscle atrophy, highlighting immediate benefits of cessation on skeletal muscles. Implications Our study demonstrates that CS-induced skeletal muscle mitochondrial dysfunction and atrophy are significantly improved by 2 weeks of cessation in male mice. We show for the first time that smoking cessation as short as 1 to 2 weeks is associated with immediate beneficial effects on skeletal muscle structure and function with the diaphragm being particularly sensitive to CS-exposure and cessation. This could help motivate smokers to quit smoking as early as possible. The knowledge that smoking cessation has potential positive extrapulmonary effects is particularly relevant for patients referred to rehabilitation programs and those admitted to hospitals suffering from acute or chronic muscle deterioration yet struggling with smoking cessation.

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Section: Animals and Study Designmentioning

confidence: 99%

Two Weeks of Smoking Cessation Reverse Cigarette Smoke-Induced Skeletal Muscle Atrophy and Mitochondrial Dysfunction in Mice

Ajimé

Serré

Wüst

et al. 2020

Nicotine &Amp; Tobacco Research

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“…C57BL/6JolaH mice (3 weeks old) were made vitamin D deficient or sufficient through the use of a vitamin D deficient or a standard control diet respectively [1] . Series 2 of [1] is elaborated in more detail below. Briefly, mice were infected with NTHi at 9 weeks of age and sacrificed at baseline and 4, 8, 24 and 72 h post-infection ( n = 6 mice/group/time point).…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Pro-inflammatory cytokines KC, Tumor Necrosis Factor (TNF)-α, Monocyte Chemotactic Protein (MCP)-1, Interleukin (IL)-6 and IL-1β were measured in the BAL fluid as described in [1] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Total RNA was extracted, converted to cDNA and analyzed via qRT-PCR as described in [1] . Primer sequences ( Table 2 ) and mRNA expression levels ( Table 3 ) of mucin glycoproteins (MUC), pattern recognition receptors (PRR), antimicrobial peptides (AMP) and surfactant proteins (SP): MUC5AC, MUC5B, toll-like receptor (TLR) 2, TLR4, lysozyme, secreted leukocyte protease inhibitor (SLPI), β-defensin 1, 2, 3 and cathelicidin related antimicrobial peptide (CRAMP), regenerating islet-derived protein 3-gamma (REG3γ), SP-A and D used in [1] are given below.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The data in this article acts as supplementary analysis that were performed in [1] . Table 1 summarizes the levels of pro-inflammatory cytokines measured in BALF before and after infection with NTHi in both groups.…”

Section: Datamentioning

confidence: 99%

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Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency

et al. 2019

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This article contains data related to the inflammatory cytokine and investigated pathways involved in bacterial clearance reported in “Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice” (Serré et al., 2018) [1]. Vitamin D deficient or sufficient mice were oropharyngeally instilled with 106 NTHi and sacrificed at 4, 8, 24 and 72 h post-infection. We measured proinflammatory cytokines (KC, TNF-α, IL-1β, IL6 and MCP-1) markers of bacterial clearance pathways (myeloid peroxidase, nitric oxide, complement C5a and immunoglobulin A) in bronchoalveolar fluid (BALF) during infection and mRNA expression levels of innate immune defense mechanism markers (mucin glycoproteins, pathogen recognitions receptor TLR2 and TLR4, antimicrobial peptides SLPI, REG3γ, lysozyme, BD-1, BD-2, BD-3 and surfactant proteins SP-A and SP-D) in lung homogenate. Finally, genomic DNA of NTHi (protein D) measured in lung homogenate was used as an indicator of NTHi invasion of alveolar macrophages or epithelial cells.

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Highlights from the 21st Workshop on Vitamin D in Barcelona, May 2018

Fleet

Campbell

Carlberg

et al. 2019

The Journal of Steroid Biochemistry and Molecular Biology

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The 21st Workshop on Vitamin D was held at the Centre de Convencions Internacional de Barcelona (CCIB) from May 16-19, 2018. The Workshop was organized by Dr Martin Hewison (Chair, University of Birmingham, Birmingham, UK) with the invaluable support of Dr JoEllen Welsh (CFO, SUNY Albany, USA) and Roxanne Hall (Meetings Plus). Invited presentations and topical sessions were chosen by the Workshop Executive Committee, with

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Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice

Cited by 15 publications

References 50 publications

Two Weeks of Smoking Cessation Reverse Cigarette Smoke-Induced Skeletal Muscle Atrophy and Mitochondrial Dysfunction in Mice

Two Weeks of Smoking Cessation Reverse Cigarette Smoke-Induced Skeletal Muscle Atrophy and Mitochondrial Dysfunction in Mice

Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency

Highlights from the 21st Workshop on Vitamin D in Barcelona, May 2018

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