Airway permeability

Persson, C. G. A.; Andersson, Morgan; Greiff, Lennart; Svensson, C.; Erjefält, Jonas; Sundler, F.; Wollmer, Per; Alkner, U.; Erjefält, I.; Gustafsson, Bàrbro; Linden, Margareta; Nilsson, Mikael

doi:10.1111/j.1365-2222.1995.tb00022.x

Cited by 49 publications

(49 citation statements)

References 42 publications

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“…Contrary to current beliefs, luminal entry of bulk plasma causes no disturbances of the pseudostratified epithelial lining, which remains uncompromised, both structurally (as assessed by light microscopy and transmission electron microscopy), and functionally (determined by absorption of small and large molecules) [2,5]. In an as yet unexplained manner, the extravasated macromolecules pass through the epithelial tight junctions situated at the apicolateral borders of the epithelial cells.…”

Section: Epithelial Passage Of Bulk Plasmamentioning

confidence: 69%

“…b) the mucosal exudation response involves little sieving of the plasma macromolecules, as indicated by similar concentration ratios between albumin (MW 69 kDa) and α 2 -macroglobulin (MW 700 kDa) in plasma and on the surface of the histamine-challenged airway. Note that histamine, even at 2,000 µg·mL -1 , may not affect the nasal mucosa as an absorption barrier [5]. Histamine inhalation and endobronchial allergen application also cause prompt bronchial mucosal exudation of α 2 -macroglobulin as determined in samples of c) induced sputum and d) bronchial lavage, respectively.…”

Section: Bioactive Molecules Of the Plasma Exudatementioning

confidence: 98%

“…Observations made in the intact tube preparation [5,32] support the above hypothesis by demonstrating that: 1) the extravasating bulk plasma moving between the columnar epithelial cells needs to increase the local hydrostatic pressure by only a few cmH 2 O to create ubiquitous paracellular passage routes for macromolecules into the lumen (paracellular stretches of 30-40 m would be available for this passage per square centimetre of mucosal surface); 2) the luminal entry operates without the need for the pharmacological effects of mediators that act directly on the epithelial barrier [32]; and 3) the hydraulic ("plasma exudate-epithelial") mechanism of luminal entry of macromolecules exhibits reversibility, repeatability and direction-selectivity in agreement with the in vivo observations [5].…”

Section: Epithelial Passage Of Bulk Plasmamentioning

confidence: 99%

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Plasma-derived proteins in airway defence, disease and repair of epithelial injury

Persson

Erjefält²,

Greiff³

et al. 1998

Eur Respir J

123

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One significant characteristic of the airway mucosa in vivo, that cannot easily be mimicked in vitro, is its microcirculation, which generates a highly dynamic, biologically active milieu of plasma-derived molecules that may pass to the airway lumen in vivo. New data on the mechanisms of airway mucosal exudation indicate that the protein systems of circulating plasma may contribute significantly to the biology and immunology of the lamina propria, its surface epithelium and the luminal surface, not only in injured airways, but also in airways that are activated but display no sign of oedema, epithelial disruption, or increased absorption capacity. We suggest that present knowledge of the mechanisms of plasma exudation, together with rapidly emerging information (not detailed herein) on receptors, target cells and cellular responses to the plasma-derived molecules, must be considered in any realistic model that investigates "immuno-inflammatory" mechanisms of the airway mucosa.

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Section: Epithelial Passage Of Bulk Plasmamentioning

confidence: 69%

Section: Bioactive Molecules Of the Plasma Exudatementioning

confidence: 98%

Section: Epithelial Passage Of Bulk Plasmamentioning

confidence: 99%

See 1 more Smart Citation

Plasma-derived proteins in airway defence, disease and repair of epithelial injury

Persson

Erjefält²,

Greiff³

et al. 1998

Eur Respir J

123

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“…Indeed, the exudation process itself has been thought to cause epithelial damage and disruption. However, this notion is inconsistent with the actual in vivo data demonstrating that bulk movement of plasma proteins to the airway lumen neither causes, nor is a reliable sign of, mucosal damage [7,8]. Acute luminal entry of bulk plasma can thus occur without disturbing the integrity of the epithelium as a tight absorption barrier in animal and human airways.…”

Section: Fig 2 (A)mentioning

confidence: 90%

“…Acute luminal entry of bulk plasma can thus occur without disturbing the integrity of the epithelium as a tight absorption barrier in animal and human airways. (Observations on the function of airway absorption barriers in vivo in acute exudative conditions as well as in chronic exudative disease with epithelial injuryrepair processes have been reviewed elsewhere [8].) Its potent molecular contents and its swift luminal entry across an intact epithelium [6] are the basis for proposing plasma exudation as part of the first-line immune defence of the airways [7].…”

Section: Fig 2 (A)mentioning

confidence: 99%

Contribution of Plasma‐Derived Molecules to Mucosal Immune Defence, Disease and Repair in the Airways

et al. 1998

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This review discusses recent observations, in health and disease, on the release and distribution of plasmaderived molecules in the airway mucosa. Briefly, the new data on airway mucosal exudation mechanisms suggest that the protein systems of plasma contribute significantly to the mucosal biology, not only in injured airways but also in such mildly inflamed airways that lack oedema and exhibit no sign of epithelial derangement. Plasma as a source of pluripotent growth factor, adhesive, leucocyte-activating, etc., molecules may deserve a prominent position in schemes that claim to illustrate immunological and inflammatory mechanisms of the airway mucosa in vivo.

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Lung proteome alterations in a mouse model for nonallergic asthma

et al. 2003

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A mouse model for nonatopic asthma was employed to study the alterations of the lung proteome to gain insight into the underlying molecular mechanisms of disease pathophysiology post-challenge. Lung samples from asthmatic and control mice were used to generate 24 high quality two-dimensional electrophoresis gels wherein 2115 proteins were examined for disease relevance. In total, 23 proteins were significantly up- or down-regulated following hapten-challenge of dinitro-fluorobenzene-hypersensitive mice. Twenty proteins were identified by mass spectrometry, of which 18 could be linked to asthma related symptoms, such as stress and inflammation, lung detoxification, plasma exudation and/or tissue remodeling. As such, proteomics was clearly vindicated as a means of studying this complex disease phenomenon. The proteins found in this study may not necessarily play a role in the immunological mechanisms and/or pathophysiology of asthma development. However, they may prove useful as surrogate biomarkers for quantitatively monitoring disease state progression or response to therapy. The mathematics of achieving statistical confidence from low numbers of gel replicates containing large numbers of independent variables stress the need for high numbers of replicates to better sample the population of proteins revealed by two-dimensional gel electrophoresis.

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Airway permeability

Cited by 49 publications

References 42 publications

Plasma-derived proteins in airway defence, disease and repair of epithelial injury

Plasma-derived proteins in airway defence, disease and repair of epithelial injury

Contribution of Plasma‐Derived Molecules to Mucosal Immune Defence, Disease and Repair in the Airways

Lung proteome alterations in a mouse model for nonallergic asthma

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