Airway proteins?surfactants

Proteomics of Human Pulmonary Surfactant Proteins

“…SP-A and SP-D play roles in host defense including response to inflammatory and infectious challenges as well as in surfactant metabolism. However, the exact mechanisms by which SP-A and SP-D work are not yet well-established (Halliday, 2004;Crouch and Wright, 2001;Hawgood et al, 2001;Floros and Wang, 2001). Using a murine model to mimic occupational exposures to endotoxin, either in the form of grain dust or purified LPS, it was observed that repeated exposure to the endotoxin caused increase of whole lung SP-A gene expression and type II alveolar epithelial cell hyperplasia, whereas SP-A level in BALF decreased (George et al, 2003).…”

“…Studies on the functions of SP-A examined under in vitro conditions indicate that SP-A functions as an autocrine regulator of phospholipids, enhances surfactant adsorption, participates in the formation of tubular myelin, regulates phospholipid turnover, blocks the phospholipid secretion, overcomes the surfactant protein inactivation of surfactant function, inhibits calcium-independent phospholipase A 2, and aids in host defense (Halliday, 2004;Hawgood and Poulain, 2001;Sano et al, 1999;Rooney, 1998;Fischer, 1998;Fischer et al, 1994;Weaver and Whitsett, 1991).…”

“…The aim of the present review is to present an outline of recent progresses in proteomic techniques and its impact on research on these proteins. Further details can be obtained from several recent review articles on the developments in proteomics technology (Noel-Georis et al, 2001Anderson and Anderson, 2002;Patton, 2002;Rabilloud, 2002;Yanagita, 2002;Zhu et al, 2003;Bischoff, 2003;Görg et al, 2004;Resing and Ahn, 2005;Wu et al, 2005) and current proteomics of pulmonary surfactant-associated proteins (Robertson et al, 1992;Pison et al, 1994;Kuroki and Voelker, 1994;Johansson et al, 1994;Vandenbussche et al, 1995;Whitsett et al, 1995;Roberston and Taeusch, 1995;Johansson and Curstedt, 1997;Pryhuber, 1998;Crouch and Wright, 2001;Hawgood and Poulain, 2001;Weaver and Conkright, 2001;Nogee, 2004;Hirsch et al, 2004;Halliday, 2004;Dietl and Haller, 2005). A general background of the pulmonary surfactant, important reviews and publications related to the developments of current proteomics of pulmonary surfactant-associated proteins, a comprehensive discussion, and the salient features of recent developments of current proteomics of pulmonary surfactant proteins follows.…”

“…With the respiratory process, tenthousand of liters of oxygen can be inhaled from the atmospheric air and carbon dioxide exhaled from the body into the external environmental air daily. The work of the respiratory process is held to a minimum due to the physiological functions of the pulmonary surfactant, a mixture of lipids and pulmonary surfactant-associated proteins, SP-A, SP-B, SP-C and SP-D (Dietl and Haller, 2005;Halliday, 2004;Hirsch et al, 2004).…”

“…The search for lung disease protein biomarkers and the formulation of a synthetic mixture for effective prophylactic and/or therapeutic application (Halliday, 2004;Hirsch et al, 2004) have, in part, been the reasons of the research work on structure and functional relationship of the lipid and protein components of the pulmonary surfactant. The latter is the proteomic studies on bronchoalveolar lavage fluid (BALF).…”