Aislamiento de levaduras killer a partir de hormigas del género Atta y su efecto sobre el hongo patógeno del tomate rojo Geotrichum candidum

Robledo-Leal, Efrén; Elizondo-Zertuche, Mariana; TreviÃ±o-Rangel, Rogelio de JesÃos; HernÃ¡ndez-Luna, Carlos; Huerta-GonzÃ¡lez, NohemÃ­

doi:10.18781/r.mex.fit.1605-3

Cited by 2 publications

References 8 publications

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Yeasts associated with the worker caste of the leaf-cutting ant Atta cephalotes under experimental conditions in Colombia

et al. 2022

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Yeasts isolated from the worker caste of the Colombian leaf-cutting ant, Atta cephalotes (Hymenoptera: Myrmicinae) were cultured and identi ed by molecular methods. Abundant, persistent and omnipresent species were classi ed as "prevalent". Experimental data were compared with information gathered from published reports on the yeast species composition in other leaf-cutting ant species. Diversity analysis was conducted using diversity values (q0, q1, and q2) to compare richness and abundance of yeasts present in different leaf-cutting ant species Clustering analysis was carried out to assess similarity of yeast community according to ant species. The yeast species composition was highly variable among the ant species. Atta laevigata and A. capiguara showed the highest degree of similarity and differed from the group composed by A. cephalotes, A. sexdens, A. sexdens rubropilosa, and A. texana. The isolation of dominant yeasts in different ant castes within the different compartments of a colony strongly suggests that the identi ed microorganisms are not transient but are native to the soil surrounding ant colonies and the substrates used by the ants to grow their fungal cultivars. It is apparent that the ant-fungus mutualism does not operate in an environment devoid of other microbes, but rather that the association must be seen within the context of a background of other microorganisms, particularly the dominant yeasts.

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Yeasts associated with the worker caste of the leaf-cutting ant Atta cephalotes under experimental conditions in Colombia

et al. 2022

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The Prevalence of Killer Yeasts in the Gardens of Fungus-Growing Ants and the Discovery of Novel Killer Toxin named Ksino

Bizarria,

Creagh,

Badigian

et al. 2024

Preprint

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Killer toxins are proteinaceous antifungal molecules produced by yeasts, with activity against a wide range of human and plant pathogenic fungi. Fungus gardens of attine ants in Brazil were surveyed to determine the presence of killer toxin-producing yeasts and to define their antifungal activities and ecological importance. Our results indicate that up to 46% of yeasts isolated from specific fungal gardens can be killer yeasts, with an overall prevalence of 17% across all strains tested. Killer yeasts were less likely to inhibit the growth of yeasts isolated from the same environment but more effective at inhibiting yeast isolated from other environments, supporting a role for killer yeasts in shaping community composition. All killer yeasts harbored genome-encoded killer toxins due to the lack of cytoplasmic toxin-encoding elements (i.e., double-stranded RNA satellites and linear double-stranded DNAs). Of all the killer yeasts identified, an isolate of Candida sinolaborantium showed a broad spectrum of antifungal activities against 57% of yeast strains tested for toxin susceptibility. The complete genome sequence of C. sinolaborantium identified a new killer toxin, Ksino, with primary and tertiary structure homology to the Saccharomyces cerevisiae killer toxin named Klus. Genome-encoded homologs of Ksino were found in yeast strains of Saccharomycetes and Pichiomycetes, as well as other species of Ascomycota and Basidiomycota filamentous fungi. This demonstrates that killer yeasts can be widespread in attine ant fungus gardens, possibly influencing fungal community composition and the importance of these complex microbial communities for discovering novel antifungal molecules.

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Aislamiento de levaduras killer a partir de hormigas del género Atta y su efecto sobre el hongo patógeno del tomate rojo Geotrichum candidum

Abstract: Resumen. Una de las enfermedades que sufre el tomate es la pudrición ácida causada por Geotrichum candidum. Para lo cual se aislaron levaduras a partir de hormigas del género Atta y se seleccio- Isolation of killer yeasts from ants of the genus

Cited by 2 publications

References 8 publications

Yeasts associated with the worker caste of the leaf-cutting ant Atta cephalotes under experimental conditions in Colombia

Yeasts associated with the worker caste of the leaf-cutting ant Atta cephalotes under experimental conditions in Colombia

The Prevalence of Killer Yeasts in the Gardens of Fungus-Growing Ants and the Discovery of Novel Killer Toxin named Ksino

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