AISLAMIENTO E IDENTIFICACIÓN DE HONGOS PATÓGENOS DE NARANJA<i>Citrus sinensis</i>L. OSBECK CULTIVADA EN BAJA CALIFORNIA SUR, MÉXICO ISOLATION AND IDENTIFICATION OF PATHOGENIC FUNGI FROM ORANGE<i>Citrus sinensis</i>L. OSBECK CULTURED IN BAJA CALIFORNIA SUR, MEXICO

Ochoa, José Luís; Hernández-Montiel, L. G.; Latisnere-Barragán, Hever; Luz, José Luis León de la; Larralde-Corona, Claudia Patricia

doi:10.1080/11358120709487712

Cited by 21 publications

(12 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fusarium culmorum is a ubiquitous soil-borne fungus able to cause root rot on citrus specimens, particularly oranges and lemons [17]. However, the most frequently isolated fungi from the rotted roots of lemon transplants were F. oxysporum , F. solani, and R. solani , showing root rot and wilt disease complexes.…”

Section: Introductionmentioning

confidence: 99%

Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

et al. 2019

View full text Add to dashboard Cite

In this study, for the environmental development, the antifungal, antibacterial, and antioxidant activities of a water extract of flowers from Acacia saligna (Labill.) H. L. Wendl. were evaluated. The extract concentrations were prepared by dissolving them in 10% DMSO. Wood samples of Melia azedarach were treated with water extract, and the antifungal activity was examined at concentrations of 0%, 1%, 2%, and 3% against three mold fungi; Fusarium culmorum MH352452, Rhizoctonia solani MH352450, and Penicillium chrysogenum MH352451 that cause root rot, cankers, and green fruit rot, respectively, isolated from infected Citrus sinensis L. Antibacterial evaluation of the extract was assayed against four phytopathogenic bacteria, including Agrobacterium tumefaciens, Enterobacter cloacae, Erwinia amylovora, and Pectobacterium carotovorum subsp. carotovorum, using the micro-dilution method to determine the minimum inhibitory concentrations (MICs). Further, the antioxidant capacity of the water extract was measured via 2,2′-diphenylpicrylhydrazyl (DPPH). Phenolic and flavonoid compounds in the water extract were analyzed using HPLC: benzoic acid, caffeine, and o-coumaric acid were the most abundant phenolic compounds; while the flavonoid compounds naringenin, quercetin, and kaempferol were identified compared with the standard flavonoid compounds. The antioxidant activity of the water extract in terms of IC50 was considered weak (463.71 μg/mL) compared to the standard used, butylated hydroxytoluene (BHT) (6.26 μg/mL). The MIC values were 200, 300, 300, and 100 µg/mL against the growth of A. tumefaciens, E. cloacae, E. amylovora, and P. carotovorum subsp. carotovorum, respectively, which were lower than the positive control used (Tobramycin 10 μg/disc). By increasing the extract concentration, the percentage inhibition of fungal mycelial was significantly increased compared to the control treatment, especially against P. chrysogenum, suggesting that the use of A. saligna flower extract as an environmentally friendly wood bio-preservative inhibited the growth of molds that cause discoloration of wood and wood products.

show abstract

Section: Introductionmentioning

confidence: 99%

Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Wood, paper, and wood products in use can be colonized by molds, causing surface discoloration when subjected to humid conditions while in use [12][13][14][15][16][17]. The phytopathogenic fungi Fusarium culmorum, F. solani, and Rhizoctonia solani have been reported to cause several diseases in plants, such as root rot and wilt disease complex [18,19], as well as causing postharvest problems for citrus and stone fruits [20,21].…”

Section: Introductionmentioning

confidence: 99%

Antifungal and Antibacterial Activities of Musa paradisiaca L. Peel Extract: HPLC Analysis of Phenolic and Flavonoid Contents

et al. 2019

View full text Add to dashboard Cite

In the present study, Melia azedarach wood samples that were treated with the methanolic extract of Musa paradisiaca L. peels were evaluated for their antibacterial and antifungal activities against Agrobacterium tumefaciens, Dickeya solani, Erwinia amylovora, Pseudomonas cichorii, Serratia pylmuthica, Fusarium culmorum, and Rhizoctonia solani. The strongest antibacterial activity was only found against A. tumefaciens (inhibition zone 90 mm), while the other bacterial strains showed resistance to wood that was treated with the extract. Potential antifungal activity against F. culmorum and R. solani was observed; the mycelial growth inhibition percentages reached 68.88% and 94.07%, respectively, in wood samples that were treated with the 3% methanolic extract of M. paradisiaca peel. HPLC analysis demonstrated the presence of seven phenolic compounds and three flavonoid compounds, as their peaks were matched with the standard compounds in a HPLC analysis. The major constituents of phenolic and flavonoid compounds in mg/100 g dry extract (DE) were ellagic acid (16.19), gallic acid (7.73), rutin (973.08), myricetin (11.52), and naringenin (8.47). The results demonstrated the potential effects of banana peel extract as a natural compound that can protect wood from molds while in use.

show abstract

“…flavus, is not a frequent pathogen of fruits, nevertheless, it has been isolated from sour lemon (Citrus aurantifolia Swingle) (Bamba & Sumball, 2005) and from orange (Citrus sinensis L. Osbeck), causando rots in postharvest (Ochoa et al, 2007), in the same manner, it has been detected in almond seeds (Prunus dulcis (Mill.) D.A.…”

Section: Fusarium Solani In Both Types Of Inflorescences Causedmentioning

confidence: 97%

“…Aspergillus niger, caused dry and humid rot in a 90 % in male inflorescences tested, over which a softening of the tissue was observed, and humid rot with abundant black sporulation that invaded the entire tissue (Table 1, Figure Revista Bio Ciencias 5(nesp1), e438. (Ochoa et al, 2007), de la misma manera, se ha detectado en semillas de almendras (Prunus dulcis (Mill.) D.A.…”

Section: Fusarium Solani In Both Types Of Inflorescences Causedmentioning

confidence: 99%

Untitled

2018

rbio

View full text Add to dashboard Cite

En México, el estado de Nayarit es el principal productor de yaca (Artocarpus heterophyllus Lam.) y recientemente el cultivo ha sido afectado por pudriciones de inflorescencias sin hasta el momento determinar el agente causal. El objetivo fue identificar el agente causal de la pudrición de inflorescencias de yaca en Nayarit y la asociación de Aphis (Toxoptera) aurantii. Durante los meses de mayo a agosto de 2016, se recolectaron inflorescencias sintomáticas con pudriciones húmedas, secas, y especímenes de pulgones asociados a inflorescencias con pudrición y micelio. Las muestras se recolectaron en huertas de yaca de los municipios de San Blas y Compostela, Nayarit, México. Se procesaron 300 disecciones de las zonas de avance de la enfermedad a partir de 250 inflorescencias y 40 disecciones de 20 especímenes de pulgones. Se aislaron seis hongos de inflorescencias y uno de pulgón, cinco se inocularon a concentración de 1 x 10 6 por mL y dos con segmentos de PDA con micelio de cinco días de edad. La inoculación se realizó con y sin herida en epidermis de inflorescencias, aparentemente sanas y en condiciones de laboratorio. Los siete hongos fueron capaces de reproducir los síntomas de pudrición del primero al quinto día post-inoculación In Mexico, Nayarit is the main state of production of jackfruit (Artocarpus heterophyllus Lam.) and recently the crop has been affected by decay of inflorescences without so far determining the causal agent. The objective of this work was to identify the cause of the jackfruit inflorescence rot in Nayarit and the association of Aphis (Toxoptera) aurantii. Symptomatic inflorescences were collected with wet, dry rot and specimens of aphids associated with inflorescences with rot and mycelium. The samples were collected in jackfruit orchards of the municipalities of San Blas and Compostela, Nayarit, Mexico. Three hundred dissections of disease progression zones were processed from 250 inflorescences and 40 dissections of 20 specimens of aphids. Six fungi of inflorescence and one of aphids were isolated; five were inoculated at a concentration of 1*10 6 per mL and two with segments of PDA with five days old Article Info/Información del artículo

show abstract

AISLAMIENTO E IDENTIFICACIÓN DE HONGOS PATÓGENOS DE NARANJACitrus sinensisL. OSBECK CULTIVADA EN BAJA CALIFORNIA SUR, MÉXICO ISOLATION AND IDENTIFICATION OF PATHOGENIC FUNGI FROM ORANGECitrus sinensisL. OSBECK CULTURED IN BAJA CALIFORNIA SUR, MEXICO

Cited by 21 publications

References 34 publications

Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

Antifungal and Antibacterial Activities of Musa paradisiaca L. Peel Extract: HPLC Analysis of Phenolic and Flavonoid Contents

Untitled

Contact Info

Product

Resources

About