Ajoene: a natural compound with enhanced antimycobacterial and antibiofilm properties mediated by efflux pump modulation and ROS generation against M. Smegmatis

Sarangi, Ashirbad; Das, Bhabani Shankar; Pahuja, Isha; Ojha, Suvendu; Singh, Vishal; Giri, Sidhartha; Bhaskar, Ashima; Bhattacharya, Debapriya

doi:10.1007/s00203-024-04189-9

Arch Microbiol

2024

DOI: 10.1007/s00203-024-04189-9

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Ajoene: a natural compound with enhanced antimycobacterial and antibiofilm properties mediated by efflux pump modulation and ROS generation against M. Smegmatis

Ashirbad Sarangi,

Bhabani Shankar Das,

Isha Pahuja

et al.

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2024

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Cited by 1 publication

References 78 publications

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Thymol as Biofilm and Efflux Pump Inhibitor: A Dual‐Action Approach to Combat Mycobacterium tuberculosis

Shankar Das,

Sarangi,

Pahuja

et al. 2024

Cell Biochemistry & Function

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Tuberculosis (TB) remains a significant global health challenge, exacerbated by the emergence of drug‐resistant strains of Mycobacterium tuberculosis (M. tb). The complex biology of M. tb, particularly its key porins, contributes to its resilience against conventional treatments, highlighting the exploration of innovative therapeutic strategies. Following with this challenges, the present study investigates the bioactivity properties of phenolic compounds derived from the terpene groups, specifically through Thymol (THY) against M. smegmatis as a surrogated model for M. tb. Furthermore, the study employed with combination of two approaches i.e., in vitro assays and computational methods to evaluate the efficacy of THY against M. smegmatis and its interaction with M. tb biofilm and efflux pump proteins, particularly Rv1258c and Rv0194. The in vitro findings demonstrated that THY exhibits inhibitory activity against M. smegmatis and shows promising interaction with a combination of isoniazid (INH) and rifampicin (RIF) of TB regimens. Furthermore, THY demonstrated significant inhibitory action towards motility and biofilm formation of M. smegmatis. The combination of THY with INH and RIF exhibited a synergistic effect, enhancing the overall antimicrobial efficacy. Additionally, THY displayed reactive oxygen species (ROS) activity and potential efflux pump inhibitory action towards M. smegmatis. The computational analysis revealed that THY interacts effectively with efflux pump proteins Rv1258c and Rv0194, showing superior binding affinity compared to verapamil, a known efflux pump inhibitor. Pharmacokinetic studies highlighted that THY possess a favourable safety profile. In conclusion, THY represents a promising inhibitory compound for tuberculosis prevention, potentially addressing challenges posed by drug resistance.

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Thymol as Biofilm and Efflux Pump Inhibitor: A Dual‐Action Approach to Combat Mycobacterium tuberculosis

Shankar Das,

Sarangi,

Pahuja

et al. 2024

Cell Biochemistry & Function

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show abstract

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Ajoene: a natural compound with enhanced antimycobacterial and antibiofilm properties mediated by efflux pump modulation and ROS generation against M. Smegmatis

Cited by 1 publication

References 78 publications

Thymol as Biofilm and Efflux Pump Inhibitor: A Dual‐Action Approach to Combat Mycobacterium tuberculosis

Thymol as Biofilm and Efflux Pump Inhibitor: A Dual‐Action Approach to Combat Mycobacterium tuberculosis

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