Akaline, saline and mixed saline–alkaline stresses induce physiological and morpho‐anatomical changes in <i><scp>L</scp>otus tenuis</i> shoots

Paz, Rosalía Cristina; Reinoso, Herminda; Espasandin, Fabiana Daniela; Antivilo, F. A. González; Sansberro, Pedro Alfonso; Rocco, Rubén Anibal; Ruíz, Oscar Adolfo; Menéndez, Ana

doi:10.1111/plb.12156

Cited by 31 publications

(15 citation statements)

References 49 publications

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“…There was an apparent change in the overall stellar region under salinity stress for both tolerant and sensitive genotypes in root and shoot of sensitive ones. Reduction in overall cross-sectional area of cortical and stellar region was observed in Lotus tenuis under salinity stress has been reported earlier [73]. The cortical cell enlargement was also observed in sensitive cultigens under salt stress.…”

Section: Discussionsupporting

confidence: 82%

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Singh

Kumari

et al. 2017

PLoS ONE

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One hundred and sixty two genotypes of different Lens species were screened for salinity tolerance in hydroponics at 40, 80 and 120 mM sodium chloride (NaCl) for 30 d. The germination, seedling growth, biomass accumulation, seedling survivability, salinity scores, root and shoot anatomy, sodium ion (Na+), chloride ion (Cl-) and potassium ion (K+) concentrations, proline and antioxidant activities were measured to evaluate the performance of all the genotypes. The results were compared in respect of physiological (Na+, K+ and Cl-) and seed yield components obtained from field trials for salinity stress conducted during two years. Expression of salt tolerance in hydroponics was found to be reliable indicator for similarity in salt tolerance between genotypes and was evident in saline soil based comparisons. Impressive genotypic variation for salinity tolerance was observed among the genotypes screened under hydroponic and saline field conditions. Plant concentrations of Na+ and Cl- at 120 mM NaCl were found significantly correlated with germination, root and shoot length, fresh and dry weight of roots and shoots, seedling survivability, salinity scores and K+ under controlled conditions and ranked the genotypes along with their seed yield in the field. Root and shoot anatomy of tolerant line (PDL-1) and wild accession (ILWL-137) showed restricted uptake of Na+ and Cl- due to thick layer of their epidermis and endodermis as compared to sensitive cultigen (L-4076). All the genotypes were scanned using SSR markers for genetic diversity, which generated high polymorphism. On the basis of cluster analysis and population structure the contrasting genotypes were grouped into different classes. These markers may further be tested to explore their potential in marker-assisted selection.

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Section: Discussionsupporting

confidence: 82%

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Singh

Kumari

et al. 2017

PLoS ONE

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“…Negative relationships between stomatal morphologies and NaCl concentration (Table 3) suggested a clear response of stomatal closure to salt in a short term for alfalfa (Paz et al, 2014;Amjad et al, 2014;Xu et al, 2015). Stomatal closure partly explained our conjecture of declined photosynthesis presented as declined growth due to pressed CO2 exchange.…”

Section: /Namentioning

confidence: 74%

“…At a larger scale among groups the heterogeneity of gas exchange, parameters is thought to partly depend on variations of stomatal number and size over the plant leaf (Weyers and Lawson, 1997;Mott and Buckley, 1998), while at a smaller scale of individual plant the gas exchange parameters was observed to be related to non-uniform stomata behavior of patchy stomatal closure Buckley, 1998, 2000). These conclusions were drawn according to studies of crop and tree plants (Paz et al, 2014;Amjad et al, 2014;Xu et al, 2015) with the correlation between stomatal closure and decline of photosynthesis (Meloni et al, 2003;Xue and Liu, 2008). However, opposed viewpoints have also been put forward based on contrasting evidence (Yang et al, 2007;Gazanchian et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…However, opposed viewpoints have also been put forward based on contrasting evidence (Yang et al, 2007;Gazanchian et al, 2007). Although leaf abaxial surface imprints have been photographed and measured to reveal morphological response of stomatal aperture to salt stress (Amjad et al, 2014;Paz et al, 2014), evidence from the results may lost certification to a great extent because current measures on stomatal aperture were generally conducted in days after salt stress when aperture length and breadth may have been generated as a result of multiple reversible changes (Hernández and Almansa, 2002). Thus, short-term measurements in the first 24 h after salt stress have not received deserved attention.…”

Section: Introductionmentioning

confidence: 99%

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Physiological Responses of Alfalfa to High-level Salt Stress: Root Ion Flux and Stomatal Characteristics

Guo

Wei²,

Zhang³

et al. 2015

IJAB

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Alfalfa is an important salt-tolerant leguminous forage-plant in salinity areas worldwide, but its performance in high level of salt stress cannot meet the satisfactory requirement. Especially, the short-term response of alfalfa to high-level salt stress is still not clear. In the present study, thirty-day-old alfalfa Gongnong No. 1 (Medicago sativa L. cv. Gongnong No.1) seedlings were exposed to NaCl treatments at concentrations of 0 (control), 50 (moderate level), 150 (high level), and 250 mM (extremely high level). Twenty-four hours after salt stress treatment, with the increase of NaCl level plant height was slightly decreased but both shoot biomass and root length were substantially declined to a dramatic extent. Also decreased was root K + concentration. In contrast, both Na + concentration and ratio of K + /Na + showed increased trends. Root K + flux was determined using non-invasive micro-test technique (NMT) around apical root tips, wherein a clear K + influx was observed at the rate of about 0.5 nmol cm -2 s -1 under the condition without salt stress, while under salt stress at the rate of 2-3 nmol cm -2 s -1 did occur K + efflux. Accordingly, stomatal length and breadth and stomatal aperture breadth decreased with the increase of NaCl concentration, while stomatal aperture density increased with time in the first 24 h after NaCl treatment. In conclusion, as a species-specific test, alfalfa is sensitive to high-level salinity with NaCl concentrations above 150 mM in the first 24 h post salt-exposure. The key mechanism was found to be presented as the pressed stamatal conductance induced by K + -Na + unbalance which was caused by root K + efflux.

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“…The pattern of morphological changes in L. tenuis root architecture after the alkaline treatment (in the absence of NaCl) was similar to that found in the mixed saltalkaline treatment and different from that observed in neutral salt. A unique root morphological response to the mixed salt-alkaline stress was the reduction in the ratio between xylem vessels and root cross-sectional areas (Paz et al 2014a). Moreover, we characterized phenotipically the response to alkaline stress of the most widely used L. japonicus ecotypes, Gifu B-129 and MG-20, and analyzed global transcriptome of plants subjected to 10 mM NaHCO 3 during 21 days, by using the Affymetrix Lotus japonicusGeneChipH .…”

Section: Abiotic Stressesmentioning

confidence: 99%

LOTUS spp: BIOTECHNOLOGICAL STRATEGIES TO IMPROVE THE BIOECONOMY OF LOWLANDS IN THE SALADO RIVER BASIN (ARGENTINA)

Antonelli¹,

Calzadilla²,

Escaray³

et al. 2016

AGR

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The Salado River Basin region is the most important livestock breeding area in Argentina, wherethe Lotus species has been traditionally cultivated as forages. Nearly 60% of their land surface is dominated by salt-affected soils with severe constraints for crop cultivation. In order to cope with that limitation, farmers have utilized species such as non-native L. tenuis (ex-Lotus glaber), which shows a very good adaptation. As a result, inter-seeding of L. tenuis has been proposed as a strategy of choice for improving forage production in marginal areas. The increase in soil quality by these means is achieved by an increment of the organic matter content, improvement of soil fertility as well as microbial biodiversity. Thus, the introduction of L. tenuis and/or other Lotus genotypes could have enormous benefits for similar constrained lands around the world. We are developing an integrated analysis of the changes that occur in soils under legume production. We will not only analyze the microbial diversity associated, but also soil physical and chemical characteristics and the impact of different legume-microbes association on mitigation of GHG emissions. In addition, we are identifying the main genetic determinants associated with interesting agronomic traits such as plant tolerance against biotic and abiotic stresses and the content of condensed tannins. Our future and present research will build a solid base for the improvement of agronomicallyimportant species and the development of better strategies for the management of constrained lands such as the lowlands in the Argentinean Pampas.

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Akaline, saline and mixed saline–alkaline stresses induce physiological and morpho‐anatomical changes in Lotus tenuis shoots

Cited by 31 publications

References 49 publications

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Physiological Responses of Alfalfa to High-level Salt Stress: Root Ion Flux and Stomatal Characteristics

LOTUS spp: BIOTECHNOLOGICAL STRATEGIES TO IMPROVE THE BIOECONOMY OF LOWLANDS IN THE SALADO RIVER BASIN (ARGENTINA)

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