AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP BAKTERI Staphylococcus aureus

Abstract

This study aims to examine the antibacterial activity of n-hexane, ethyl acetate, and n-butanol extract of tenggulun leaves (Protium javanicum Burm. F.) in inhibiting the growth of S. aureus bacteria and determine the minimum inhibitory concentration of the most active extracts and identify its active compounds. A total of 1000 g of tenggulun leaf powder was macerated with methanol and 91.78 g of crude methanol extract was obtained which was further partitioned into n-hexane, ethyl acetate, and n-butanol extracts. Antibacterial activity test was carried out using the diffusion well method and the result showed that the ethyl acetate extract at a concentration of 20% was strongly able to inhibit the growth of S. aureus bacteria with inhibition zones of 20.08 mm, followed by n-butanol 16.66 mm, and n-hexane 13.33 mm. The minimum concentration of ethyl acetate extract was 0.2% in inhibiting the growth of S. aureus with inhibition zone diameter of 6.20 mm. Separation of the active ethyl acetate extract was conducted by silica gel column chromatography with mobile phase of n-hexane: ethyl acetate (7.5: 2.5) gave 6 combined fractions. Fractions B, G, H and I showed antibacterial activity at concentrations of 50% with a diameter of inhibition zone of 5.62 mm, 6.87 mm, 8.50 mm, and 6.75 mm respectively. The results of mass spectra analysis from the chromatogram peaks of LC-MS / MS suggested that the G and I fractions were tentatively identified as crotarin, benzophenone-2, and medroxyprogesterone acetate which may contributed to the antibacterial activity by acting synergisticly. 
 
 Keywords: antibacterial, tenggulun, Staphylococcus aureus, LC-MS/MS.

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“…Salah satu tumbuhan yang memiliki potensi sebagai antioksidan alami adalah Tenggulun (Protium javanicum Burm f.). Tumbuhan tenggulun dilaporkan mengandung flavonoid, terpenoid, steroid, fenol, saponin, dan tanin yang berpotensi sebagai antioksidan (Puspawati, et al, 2023). Pada penelitian yang dilakukan oleh Adfa et al (2013)…”

EFEK PEMBERIAN EKSTRAK KAYU CENDANA (Santalum album L.) TERHADAP KADAR C-REACTIVE PROTEIN (CRP) TIKUS PUTIH (Rattus norvegicus) MODEL SEPSIS Methicillin-Resistant Staphylococcus aureus (MRSA)

“…Kayu cendana juga mengandung senyawa yang digunakan sebagai antiinflamasi (Puspawati Ni Made, et al, 2017). Kandungan senyawa fitokimia tersebut dapat berupa senyawa alkaloid, terpenoid, flavonoid, fenol, tanin, dan steroid yang berpsotensi sebagai senyawa untuk antibakteri (Puspawati, 2018).…”

UJI TOKSISITAS EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

“…Kajian ilmiah tentang khasiat daun tenggulun lebih banyak dilaporkan pada minyak atisrinya (Sukmajaya et al, 2012;Suirta et al, 2016;dan Setianingsih et al, 2013). Ekstrak metanol daun tengulun dilaporkan memiliki aktivitas sebagai antiinflamasi (Ahuja et al, 2019), sebagai antibakteri (Puspawati et al, 2020) dan beberapa penelitian melaporkan bioaktivitas ekstrak kulit batang tenggulun sebagai antiinflamasi (Dewi et al, 2015dan Suirta et al, 2016.…”