Aktivitas antibakteri Pleurotus ostreatus varietas Grey Oyster pada Staphylococcus aureus dan Pseudomonas aeruginosa

Abstract


 Oyster mushroom Pleurotus ostreatus varieties Gray oyster is often consumed by the community because it tastes delicious and contains nutrients for health but utilization for medical treatment has not been studied. The aim of this research was to analyze antimicrobial potential compounds in P. ostreatus and to investigate the inhibitory effect on the growth of Staphyllococcus aureus and Pseudomonas aeruginosa. This research was experimental type with treatment variation 0.1; 0.25; 0.5; 1; 1.5 and 2% of P. ostreatus extract, with 5 replicates. Antimicrobial potential compounds was analyzed by qualitative phytochemical test while antibacterial activity evaluated by calculating the inhibition zone diameter presented as the bright zone formed in the surroundings paper discs on bacterial cultures using solid medium. Phytochemical analysis obtained positive results for flavonoid, tannin and terpenoid compounds. The effect of mushroom extract on the bacterial growth inhibition were analyzed by One Way Anova test. The result showed that the inhibitory growth diameter for both bacteria was not significantly different. It meant that the difference in concentration variations of P. ostreatus extracts of 0.25 and 0.50 did not cause different inhibitory effects on S. aureus and P aeruginosa bacteria and 0.1% concentration was capable of causing inhibitory effect.

Review: SNEDDS Gel Kombinasi Ekstrak Daun Pegagan dan Daun Sirih Merah Sebagai Agen Penyembuh Luka

“…Uji aktivitas antibakteri metode difusi memiliki indikator diameter zona hambat, dimana semakin besar diameter zona hambat maka aktivitas antibakteri semakin baik. Kategori diameter zona hambat berdasarkan penelitian Hanizar dan Sari [21], adalah (1) nilai zona hambat ≤ 5 mm yaitu lemah, (2) nilai zona hambat 6-10 mm tergolong sedang, (3) nilai zona hambat 11-20 mm tergolong kuat, dan (4) nilai zona hambat ≥ 21 mm tergolong sangat kuat. Data terkait aktivitas antibakteri daun sirih merah sebagai agen penyembuh luka ditunjukan oleh tabel 4.…”