Aktivitas Bakteri Endofit Asal Padi Lokal Kamba Dalam Menghambat Pertumbuhan Koloni Alternaria Porri  Secara in Vitro

Sudewi, Sri; Ratnawati, Ratnawati; Bangkele, Lisa Indriani; Idris, Idris; Jaya, Kasman; Saleh, Abdul Rahim

doi:10.35329/agrovital.v7i1.2856

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…began to grow to fill the petri dish on the 9th day of observation. The research conducted by Sudewi stated that, the time needed for A. porri to grow to fill the petri dish is ±14 days [16].…”

Section: Alternaria Sp Colony Growth Rate (Mm/day)mentioning

confidence: 99%

Test antagonism of several Trichoderma species in suppressing the growth of Alternaria sp. in vitro

Susanna,

Sayuthi,

Salsabila

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Alternaria sp. is a pathogenic fungus that causes purple blotch disease in onion plants. Losses from this disease lead to a decrease in yield by more than 70% and seed damage to 100%. For this reason, a study was conducted to test the antagonistic ability of several Trichoderma species in suppressing the growth of Alternaria sp. in-vitro as an alternative to plant disease control that is safer and more environmentally friendly. Alternaria sp. isolate. derived from onion plants located in Bale Atu, Bukit District, Bener Meriah Regency, Aceh Province. The Trichoderma species used are T. asperellum, T. harzianum, T. virens, and Trichoderma sp. Antagonism testing was carried out using a dual culture method in a 9 cm diameter petri dish. The results showed that, T. asperellum, T. harzianum, T. virens, and Trichoderma sp. able to suppress the growth of Alternaria sp. on observation of 5 HSI. The antagonistic mechanism that occurs is competition against space, nutrition, antibiosis and mycoparasites. The best percentage of resistance occurred in the treatment of T. virens, which was 77.78%. next followed by the inhibitory power of Trichoderma sp. by 72.39%, T. harzianum by 64.80%, and T. asperellum by 60.77%.

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Section: Alternaria Sp Colony Growth Rate (Mm/day)mentioning

confidence: 99%

Test antagonism of several Trichoderma species in suppressing the growth of Alternaria sp. in vitro

Susanna,

Sayuthi,

Salsabila

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…The isolation followed the protocol suggested previously [13]. Initially, the leaves were clean washed using distilled water and cut into 1 cm 2 , before soaked in ethanol 70% for 1 min.…”

Section: Isolation Of Fungal Pathogens From Shallotsmentioning

confidence: 99%

Antagonistic activity of Trichoderma sp. against pathogens in the leaves of Allium ascalonicum L.

Maulana,

Lubis,

Harahap

et al. 2024

Narra X

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Pathogenic fungi pose constraints and reduce shallot production. Trichoderma sp. is an antagonistic fungus capable of controlling pathogen growth in shallots. The aim of this study was to determine the characteristics of Trichoderma sp. and pathogens in shallots and to assess the antagonistic ability of Trichoderma sp. against shallot pathogens (Allium ascalonicum L.). Trichoderma sp. and shallot pathogens were isolated using a serial dilution agar plate method using potato dextrose agar medium with 7-day incubation. The antagonistic activity of Trichoderma sp. against shallot pathogens was evaluated based on the dual culture method. In this study, we successfully isolated Trichoderma harzianum from the shallot leaf and its root systems. Moreover, four morphologically distinctive pathogens from shallot roots and leaves were successfully isolated (l Aspergillus sp., Colletotrichum sp., Phytium sp. and Penicillium sp. T. harzianum was found to have the ability to inhibit 23.45% growth of Aspergillus sp, 26.19% growth of Colletotrichum sp., 75.40% growth of Phytium sp., and 40.38% growth of Penicillium sp. In conclusion, the isolated T. harzianum had a strong antagonistic activity against some pathogens in the shallot, but the activity was weak against some others.

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“…Upaya pengendalian yang dapat dilakukan yaitu dengan penggunaan fungisida sintetik. Namun penggunaan fungisida sintetik untuk mengendalikan penyakit ini perlu diaplikasikan dengan frekuensi tinggi (Sudewi et al, 2022). Aplikasi yang tidak disertai dengan dosis yang tepat, intensitas berlebihan dapat menyebabkan pencemaran lingkungan dan resistensi dari cendawan patogen A. porri tersebut (Sari et al, 2017).…”

Section: Pendahuluanunclassified

“…Cendawan patogen A. porri dalam penelitian ini adalah isolat koleksi Ratnawati et al (2019) hasil isolasi dari tanaman bawang merah "Lembah Palu" yang menunjukkan gejala penyakit bercak ungu. Miselium isolat cendawan patogen murni hasil koleksi disubkultur kembali pada media PDA steril, lalu diinkubasikan pada suhu 28 o C, untuk selanjutnya digunakan sebagai isolat uji (Sudewi et al, 2022).…”

Section: Penelitian Dilaksanakan DIunclassified

Isolasi dan karakterisasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” dan potensinya menghambat penyakit bercak ungu Alternaria porri (ELL) CIF

Sudewi,

Ratnawati,

Jaya

et al. 2023

J. AGRO

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The decrease in shallot production due to Alternaria porri infection can reach 50%-70% or can even cause crop failure. One of the biological control methods to overcome this is by utilizing antagonistic microbes. The purpose of this study was to identify endophytic fungi from the rhizosphere of shallots "Lembah Palu " that have the potential to inhibit the A. porri fungus that causes purple spot disease. Research January-September 2022, the activity began with isolating endophytic fungi using the surface sterilization method, followed by characterization and identification of morphology macroscopically and microscopically, compatibility testing between isolates, and antagonism power test. The results obtained 5 isolates of endophytic fungi identified in the genus Fusarium sp., Aspergillus flavus, Penicillium sp., Aspergillus niger, and Trichoderma sp. A total of seven compatible interactions between endophytic fungi resulted from compatibility tests, while 3 other interactions were not compatible. The antagonism test of 5 fungal isolates against the A. porri produced an average inhibition of ≥ 75%. The HKP5 isolate (Penicillium sp) had the best inhibition of 78.96%. This indicates that endophytic fungal isolates have some potencies the potential to be developed as biological control agents in an effort to support sustainable agriculture.ABSTRAKPenurunan produksi tanaman bawang merah akibat serangan Alternaria porri dapat mencapai 50%-70% bahkan dapat menyebabkan terjadinya gagal panen. Salah satu metode pengendalian hayati untuk mengatasi hal tersebut yaitu dengan memanfaatkan mikroba yang bersifat antagonis . Tujuan dari penelitian ini untuk mengidentifikasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” yang berpotensi dalam menghambat cendawan A. porri penyebab penyakit bercak ungu. Penelitian Januari-September 2022, kegiatan diawali dengan mengisolasi cendawan endofit menggunakan metode sterilisasi permukaan, dengan perendaman sampel akar berurutan dalam 2% Natrium hipoklorit, etanol 70% dan air steril, kemudian dikulturkan dalam media Potato Dextrose Agar (PDA). Selanjutnya setelah isolat tumbuh, disubkultur kembali untuk dilakukan karakterisasi dan identifikasi morfologi secara makroskopis dan mikroskopis, pengujian kompatibilitas antarisolat serta uji daya antagonisme. Dari hasil penelitian diperoleh 5 isolat cendawan endofit yang teridentifikasi dalam genus Fusarium sp., Aspergillus flavus, Penicillium sp., Aspergillus niger, dan Trichoderma sp. Sebanyak tujuh interaksi kompatibel antarcendawan endofit dihasilkan dari uji kompatibilitas, sedangkan 3 interaksi lainnya tidak kompatibel. Uji daya antagonisme 5 isolat cendawan terhadap patogen A. porri menghasilkan rata-rata daya hambat ≥ 75%. Isolat HKP5 (Penicillium sp) memiliki daya hambat terbaik sebesar 78,96%. Hal ini mengindikasikan bahwa isolat cendawan endofit berpotensi untuk dikembangkan sebagai agens pengendali hayati dalam upaya mendukung pertanian berkelanjutan.

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Aktivitas Bakteri Endofit Asal Padi Lokal Kamba Dalam Menghambat Pertumbuhan Koloni Alternaria Porri Secara in Vitro

Cited by 4 publications

References 11 publications

Test antagonism of several Trichoderma species in suppressing the growth of Alternaria sp. in vitro

Test antagonism of several Trichoderma species in suppressing the growth of Alternaria sp. in vitro

Antagonistic activity of Trichoderma sp. against pathogens in the leaves of Allium ascalonicum L.

Isolasi dan karakterisasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” dan potensinya menghambat penyakit bercak ungu Alternaria porri (ELL) CIF

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