Akut Lenfoblastik Lösemide Flow Sitometri ile İmmünofenotiplemenin Prognoz Üzerine Etkileri

Abstract

Background: The identification of immunophenotype subgroups is very important for the diagnosis and prognosis of acute lymphoblastic leukemia (ALL). Material and Method:The study is designed as a retrospective cohort study which included 105 children with ALL (65 males, 40 females; mean age 5.9±3.8 years) who were treated TR-ALL 2000 (modified) BFM treatment protocol. Results:The distributions of EGIL classification were pro-B ALL (n=1), common B ALL (n=46), pre-B ALL (n=40), pre-T ALL (n=8), cortical T ALL (n=6), and mature T ALL (n=4). Leukocyte≥100,000/ mm³, lymphadenopathy≥2 cm, mediastinal involvement were commonly identified in T ALL group. T ALL had a poor response to chemotherapy according to 8th-day peripheral circulation blast counts and 15th-day bone marrow aspiration (BMA) blast counts. The recurrence, mortality, and death rate in the induction period of treatment were frequently detected in T ALL group. The variables that had prognostic potential, as indicated by univariate analyses, were leukocyte count, hepatomegaly, splenomegaly, and lymphadenopathy at the time of diagnosis, 8th-day steroid response, 15th-day BMA response, risk group, recurrence, and immunophenotyping. Multivariate Cox regression analysis demonstrated that only the leukocyte count (HR 2.51, p < 0.001) was a predictor of prognosis. Conclusion:Immunophenotyping may be effective in the diagnosis and prognosis of ALL, identification of risk groups, and in risk-based treatment planning. T ALL had a poor prognosis.

Evaluation of the Correlation of Immunohistochemical Findings with Flow Cytometric Findings in Newly Diagnosed Pediatric Acute Lymphoblastic Leukemia Patients

“…In terms of gender distribution in ALL cases in the literature, it was observed as 55.6% male, 44.4% female and 61.9% male, 38.1% female, [27] and it is more common in male. [28] In our study, 71.42% of the cases were male and 28.58% were female, and our cases in the male gender were higher compared to the literature. In the study of Noronha et al [25] the mean age of BCP-ALL cases was four years and the mean age of T-ALL cases was 8 years and in our study, the mean age of BCP-ALL cases (between 2 and 17 years) was 7.22, the age of T-ALL cases was between 3 and 14, with a mean age of 8.80.…”