AL-002 Effects of sperm preparation on sperm DNA fragmentation and morphology

Efficacy of swim-up versus density gradient centrifugation in improving sperm deformity rate and DNA fragmentation index in semen samples from teratozoospermic patients

“…Jayaraman et al pointed out that the percentage of TUNEL positive sperm after sperm processing by swim-up or DGC was significantly lower in teratozoospermic samples, and there was no difference in the incidence of TUNEL positive sperm between the two techniques, suggesting that the two techniques are comparable in terms of recovering sperm with low DNA damage [14]. In addition, controversial results also exist concerning the selection ability and effect of different kinds semen preparation methods that could recover the maximal number of sperm with genetic competence [22][23][24]. These controversial results indicate that a wide variety of factors may affect sperm DNA integrity, including semen selection method, DNA integrity evaluation technique, and study population.…”

Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

“…It has been reported that although discontinuous gradient eliminates morphologically abnormal sperm and swim-up treatment decreases DFI and HDS of spermatozoa, both methods are effective for embryo development [18]. The first study to compare the effects of gradient-density centrifugation and swim-up techniques on sperm apoptosis using flow cytometry also suggested that both the sperm preparation methods allow obtaining a sperm population with low percentage of apoptotic sperm [19].…”

“…Comparative studies done earlier on sperm preparation methods have essentially investigated outcomes such as recovery rates and conventional semen parameters [9][10][11][12][13]. In the recent days, many studies have been conducted, attempting to identify an effective sperm preparation method that would yield the maximal number of genetically competent sperm [14][15][16][17][18][19]. The results of these studies, are however inconclusive to recommend any specific selection method [20,21].…”

Hartree–Fock interactions in the integer quantum Hall effect

“…In Figs. 2 and 3 we show results for a model of an n-type InSb structure of three-dimensionally distributed donor atoms with a 2DES sitting on top of the cleaved surface [42]. The first 2 spin-split Landau levels are shown for…”

“…The local density of states (LDOS) is a very interesting quantity which is experimentally accessible via spatial scanning tunneling spectroscopy (STS) [40][41][42]. The differential tunneling current between the STS tip and the sample is thereby proportional to the density of existing states at a certain energy.…”