AL-003 Effects of removal of necrotic blastomeres from human cryopreserved embryos on pregnancy outcome

Wx, Liu; Huang, Pinxiu; Wang, L; Cy, Zhao; Luo, MJ

doi:10.1016/s1472-6483(11)60493-8

Cited by 3 publications

(3 citation statements)

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“…However, laser techniques can facilitate the removal of necrotic blastomeres from the embryo by aspiration via a small hole created by a laser through the zona pellucida. Removal of necrotic blastomeres from partially damaged embryos has been shown to significantly increase the developmental potential of partially damaged frozen-thawed embryos; indeed, clinical pregnancy rates and implantation rates more than double those ensuing when necrotic blastomeres are not removed have been reported (Liu et al, 2005(Liu et al, , 2006Rienzi et al, 2002). Rienzi and colleagues showed that the developmental potential of partially damaged frozen-thawed embryos can be equivalent to normally healthy embryos if necrotic blastomeres are removed with the aid of laser technology, even if multiple blastomeres need to be removed (Rienzi et al, 2005).…”

Section: Embryo Cryopreservation Removing Necrotic Blastomeresmentioning

confidence: 99%

Laser technology in the ART laboratory: a narrative review

Davidson

Liu

Griffiths

et al. 2019

Reproductive BioMedicine Online

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This review discusses the current status of laser-assisted reproductive technologies in human infertility treatment. We highlight the benefits of laser technology in diagnostic and therapeutic treatments for subfertility and male-factor infertility, and current concern of this application in different clinical practice. ABSTRACTTo improve success rates, assisted reproductive technology (ART) procedures continually undergo optimization and enhancement such that the best quality gametes and embryos can be identified and manipulated, thus improving clinical outcomes. Laser technology is now being applied across ART to reduce procedure times and increase the consistency and reproducibility of traditional ART techniques such as assisted hatching, embryo biopsy, intracytoplasmic sperm injection cryopreservation and sperm immobilization/selection. This review examines the current status of cutting-edge laser-assisted reproductive technologies, investigates experimental techniques that are increasingly being applied clinically. It highlights the benefits of lasers as a powerful technology at the forefront of both diagnostic and therapeutic treatments for general subfertility and male-factor infertility. However, it is important to note that although lasers are becoming increasingly commonplace in ART units, there is comparatively little information in the existing literature pertaining to the potential negative effects that laser application might have on the developing human embryo, thus creating the need for further investigative research.

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Section: Embryo Cryopreservation Removing Necrotic Blastomeresmentioning

confidence: 99%

Laser technology in the ART laboratory: a narrative review

Davidson

Liu

Griffiths

et al. 2019

Reproductive BioMedicine Online

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“…It can be concluded that the developmental potential of partially damaged frozen and thawed embryos can be equivalent to fully intact embryos if the necrotic blastomeres are removed from the partially damaged embryos and only those of that showed post-thaw cleavage are selected for transfer (Rienzi et al, 2005;Liu et al, 2007).…”

Section: Removal Of Detrimental Structuresmentioning

confidence: 99%

Micromanipulation Techniques in IVF

Ebner

2010

Journal of Reproductive and Stem Cell Biotechnology

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After the world witnessed groundbreaking early advances in assisted reproduction that utilized conventional in vitro fertilization in the human, more complex invasive techniques of oocyte and embryo manipulation were introduced to treat human subfertility. Amongst these were numerous micromanipulation techniques such as partial zona dissection (PZD; 1988), subzonal insemination (SUZI, 1988) and subsequently ICSI, which became the most powerful tool of assisted fertilization (1992). ICSI requires micromanipulation of both the male and the female gamete. Another important approach is laser-assisted opening of the zona pellucida at different stages of preimplantation development either; to assist hatching (1990) or to remove cells for preimplantation genetic diagnosis (1989), or fragments to restore the integrity of an embryo (1999). Sometimes even blastocysts considered for vitrification need further manipulation in order to artificially shrink the blastocoel which facilitates survival of expanded blastocyst (2002). Other methods for reproductive purposes, however, failed to gain acceptance in population, such as cytoplasm transfer (1997), nuclear transfer (1998) and cloning (1996), to name but a few.

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“…Microsurgical removal of degenerated blastomeres of embryos was first carried out by Alikani et al (1993). This method was applied to human embryos, cryopreserved using slow cooling rates (Rienzi et al, 2005b;Elliott et al, 2007;Liu et al, 2007). The removal of non-compacted morulae cells and fragmentations .…”

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confidence: 99%

Cryopreservation of incomplete compacted morulae and preliminary biopsy of excluded fragments

Petrushkо

Yurchuk

Piniaiev

et al. 2019

Zygote

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SummaryThe complexity of predicting embryo development potential at the cleavage stages and the emergence of epigenetic risks during prolonged in vitro culture of pre-implantation embryos made it more advantageous to transfer embryos at the morula stage to the uterine cavity. The criteria for estimating embryos at this stage that allow prediction of cryopreservation outcomes have been poorly described. All day 4 embryos (n = 224) were graded 1, 2, 3, 4 or 5 according to blastomere compaction degree (BCD = 100, 75, 50, 25 or 0%, respectively) and the survival and blastocyst formation rate of these morulae were studied after cryopreservation. An inverse dependence was found between survival rate and BCD. Excluded fragments were characterized by low osmotic reaction during exposure to cryoprotective medium and, after freeze-thawing, they were destroyed. As damaged necrotic areas of the embryo can affect their further development rate we proposed blastomeres and biopsy fragments of incomplete compacted morula be removed before embryo cryopreservation. This step led to significant increase in the post-thawing survival rate up to 93.1 ± 4.1%, 75 ± 8.8% and blastocyst formation rate up to 85.2 ± 10.4%, 59.4 ± 5.2% in grade 2 and grade 3 embryos, respectively. There was no significant difference in grade 4 embryos. Therefore the removal of blastomeres and biopsy fragments in incomplete compacted morulae can improve cryopreservation outcomes of grade 2 and grade 3 embryos with BCD.

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AL-003 Effects of removal of necrotic blastomeres from human cryopreserved embryos on pregnancy outcome

Cited by 3 publications

References 0 publications

Laser technology in the ART laboratory: a narrative review

Laser technology in the ART laboratory: a narrative review

Micromanipulation Techniques in IVF

Cryopreservation of incomplete compacted morulae and preliminary biopsy of excluded fragments

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