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Abstract

Proliferative activity and lipid composition (phospholipids and gangliosides) were studied in rat hepatoma-27 transplanted subcutaneously or intrahepatically (as models for primary and metastasizing tumors). The mitotic index of subcutaneously transplanted hepatoma far exceeded that of the intrahepatically transplanted tumor. The overall amounts of both phospholipids and gangliosides increased appreciably in the subcutaneously growing hepatoma (in contrast to the intrahepatically growing tumor) in comparison to the control hepatic tissue. The ganglioside composition in the tumors differs from that in the liver: ganglioside GD3 appears, whereas gangliosides GD1b and GT1b decrease in amount in the intrahepatic tumor compared to the control liver and disappear in the subcutaneously transplanted hepatoma. In both tumor types, the amounts of both phosphatidylethanolamine and sphingomyelin exceed the control values. Comparison of these results with previously reported data concerning the phospholipid and ganglioside composition in the regenerating rat liver indicates that the difference in the lipid composition between the subcutaneously and intrahepatically growing hepatomas-27 is due to their different proliferative status and also their microenvironment.

Metabonomic Studies of Human Hepatocellular Carcinoma Using High-Resolution Magic-Angle Spinning 1H NMR Spectroscopy in Conjunction with Multivariate Data Analysis

“…The elevation of PE level is consistent with what was reported from a study of the rat hepatic tumors, 40 and the changes of choline metabolites are also in good agreement with previously reported results in in vivo MRS studies of both human HCC tumors 11,14 and rat models. 2,9,10,13 The elevation of PE and choline metabolites in HCC tumors probably resulted from metabolism of the membrane phospholipids due to accelerated cell proliferation, being consistent with the elevation of the total cholines in malignant tumors 17,18,20 and liver lesions.…”

“…The elevation of PE level is consistent with what was reported from a study of the rat hepatic tumors, and the changes of choline metabolites are also in good agreement with previously reported results in in vivo MRS studies of both human HCC tumors 11,14 and rat models. ,,, The elevation of PE and choline metabolites in HCC tumors probably resulted from metabolism of the membrane phospholipids due to accelerated cell proliferation, being consistent with the elevation of the total cholines in malignant tumors 17,18,20 and liver lesions. , Such event may also induce obstruction of bile ducts, leading to the increase of bile acid signal in tumors. However, the changes of lactate, glycine, leucine, alanine, glutamate, and glutamine were not reported before in HCC tumors due to low sensitivity and resolution and probably resulted from promotion of glycolysis and disruption of TCA cycle.…”

Efficacy of Withania somnifera on lipid profile of endosulfan induced toxicity in Swiss albino mice

“…Endosulfan also causes oxidative damage to the membrane due release of ROS leading to lipid peroxidation (Hincal et al, 1995;El-Shenawy 2010;Oliveira et al,2017). This altogether causes the onset of pathogenesis, causing disease in the animal (Di Rosa et al 1971;Kobliakov et al,2001;Sood et al 2009). Endosulfan is a known xenoestrogen which causes severe damage to the membrane due to lipid peroxidation.…”