Alanyl-Glutamine (Ala-Gln) Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Colitis by Regulating the Gut Microbiota, PI3K-Akt/NF-κB/STAT3 Signaling, and Associated Pulmonary Injury

Abstract

The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice. The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4 + T-cell subsets in mesenteric lymph nodes (MLNs). In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS-induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes. Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis. Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4 + T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis. In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.

Oral vancomycin treatment alters levels of indole derivatives and secondary bile acids modulating the expression of mTOR pathway genes in astrocytes during EAE

“…These findings suggest that Clostridia vadinBB60 group may have anti-inflammatory properties. Consistent with this notion, two studies have reported that Clostridia vadinBB60 group is protective in IBD 42,43 .…”

Preparation of Glutamine-Enriched Fermented Feed from Corn Gluten Meal and Its Functionality Evaluation

“…Currently, a greater diversity and richness in gut bacteria are considered advantageous, helping animals navigate various environmental challenges. Gln and glutamine peptides have been reported to preserve intestinal structure and function, in addition to enhancing growth performance, by modulating gut microbiota [34]. In this study, microbial diversity, richness, and community abundance experienced a significant upsurge when GCFF replaced 15% and 30% of SBM (Figure 2).…”

Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites

“…AESc upregulated the abundance of beneficial Alcaligenaceae (Collins et al, 2014), Coriobacteriaceae (Sun et al, 2023), Ruminococcaceae (Keshteli et al, 2022), Lachnospiraceae (Herp et al, 2019), [Paraprevotellaceae], Mucispirillum (Herp et al, 2019), Adlercreutzia (Chen et al, 2019), Allobaculum (van Muijlwijk et al, 2021, [Ruminococcus] (Lee et al, 2017), and [Prevotella] (Iljazovic et al, 2021) and downregulated the abundance of pathogenic Turicibacteraceae (Tong et al, 2021), Enterobacteriaceae (Hu et al, 2022), Proteus (Zhang et al, 2021), Turicibacter (Meng et al, 2019), Coprococcus (Yang et al, 2023), Shigella (Dekker and Frank, 2015), and Prevotella (Sharma et al, 2022). However, the modulating effects on Desulfovibrionaceae (Liu et al, 2023), Deferribacteraceae (Berry et al, 2012), Helicobacteraceae Tong et al, 2021), Verrucomicrobiaceae (Tong et al, 2021), Bacteroidaceae (Peng et al, 2019), Lactobacillaceae (Ma L. et al, 2022), Prevotellaceae (Liu et al, 2020), Oscillospira (Chen et al, 2021), Akkermansia (Qu et al, 2021), Bacteroides (Shao et al, 2021), and Lactobacillus (Jia et al, 2022) were inconsistent with the literature reports. We hypothesized that AESc regulates the UC intestinal flora selectively.…”