ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules

Tong, Jingjing; Ren, Zhitong; Sun, Landi; Zhou, Sixian; Yuan, Wei; Hui, Yufan; Ci, Dong; Wang, Wei; Fan, Liu‐Min; Wu, Zhe; Qian, Weiqiang

doi:10.1038/s41477-022-01175-1

Cited by 50 publications

(28 citation statements)

References 59 publications

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“…For many years, mRNA decay appears to be an important process for plant development and response to stress [ 3 , 8 , 19 , 20 , 21 , 22 ]. The development of accurate methods to quantify decay intermediates is crucial to understand the importance of mRNA turnover in gene expression regulation.…”

Section: Discussionmentioning

confidence: 99%

Monitoring mRNA Half-Life in Arabidopsis Using Droplet Digital PCR

Boubegtitene

Merret

2022

Plants

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mRNA decay is an important process in post-transcriptional regulation; in addition, it plays a crucial role in plant development and response to stress. The development of new tools to quantify mRNA decay intermediates is thus important to better characterize the dynamic of mRNA decay in various conditions. Here, we applied droplet digital PCR (ddPCR), a recent and precise PCR technology, to determine mRNA half-life in Arabidopsis seedlings. We demonstrated that ddPCR can correctly assess mRNA half-life from a wide variety of transcripts in a reproducible manner. We also demonstrated that thanks to multiplexing mRNA, the half-life of multiple transcripts can be followed in the same reaction. As ddPCR allows precise quantification, we proposed that this approach is highly suitable when a low amount of RNA is available; for the detection of many targets or for the analysis of lowly expressed transcripts.

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Section: Discussionmentioning

confidence: 99%

Monitoring mRNA Half-Life in Arabidopsis Using Droplet Digital PCR

Boubegtitene

Merret

2022

Plants

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“…Furthermore, expansion of the SYMPL toolbox can also open the door for detecting and monitoring a wide assortment of post-translational modifications, such as ubiquitination or acetylation, by swapping the FHA1/WW/14-3-3 phospho-binding domain with a ubiquitin or acetyl-Lysine binding domain 69 70 71 . Additionally, this approach can be adapted to study other types of biomacromolecule interactions involving for instance protein-RNA recognition 12 72 . In vitro studies were even able to specifically visualize phase-separated protein-methylated DNA interaction 73 .…”

Section: Discussionmentioning

confidence: 99%

“…Phase separation relies on the presence of intrinsically disordered regions in proteins and occurs naturally in biological systems, such as stress granules, nucleoli and Cajal bodies 7 8 9 . Furthermore, it was shown to control numerous physiological processes such as immunity, flowering, auxin signaling, seed germination, CO2 sensing, thermotolerance, endocytosis, transposon silencing and RNA processing 10 11 12 13 14 15 . For example, AUXIN RESPONSIVE FACTORs (ARFs) undergo phase separation through their intrinsically disordered PB1 domains leading to the formation of a large complex unable to access the nucleus.…”

Section: Mainmentioning

confidence: 99%

Phase separation-based visualization of protein-protein interactions and kinase activities in plants

Safi

Smagghe

Gonçalves

et al. 2022

Preprint

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Protein activities depend heavily on protein complex formation and dynamic post-translational modifications, such as phosphorylation. Their dynamic nature is notoriously difficult to monitor in planta at cellular resolution, often requiring extensive optimization and high-end microscopy. Here, we generated and exploited the SYnthetic Multivalency in PLants (SYMPL)-vector set to study protein-protein interactions (PPIs) and kinase activities in planta based on phase separation. This technology enabled easy detection of inducible, binary and ternary protein-protein interactions among cytoplasmic, nuclear and plasma membrane proteins in plant cells via a robust image-based readout. Moreover, we applied the SYMPL toolbox to develop an in vivo reporter for SnRK1 kinase activity, allowing us to visualize tissue-specific, dynamic SnRK1 activation upon energy deprivation in stable transgenic Arabidopsis plants. The applications of the SYMPL cloning toolbox lay the foundation for the exploration of PPIs, phosphorylation and other post-translational modifications with unprecedented ease and sensitivity.

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“…O t h e r D N A / R N A -b i n d i n g p r o t e i n s i n c l u d e ACETYLATION LOWERS BINDING AFFINITY (ALBA) proteins, which are well studied in archaea and protozoan parasites. Scientists have also identified six types of ALBA proteins in Arabidopsis, of which ALBA4, ALBA5, and ALBA6 especially help plants fight heat stress (Tong et al, 2022). Under normal conditions, ALBA proteins can interact with many SG and P-body components, including DCP5 and Rbp47.…”

Section: Cytoplasmic Inducible Condensatesmentioning

confidence: 99%

Landscape of biomolecular condensates in heat stress responses

et al. 2022

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High temperature is one of the abiotic stresses that plants face and acts as a major constraint on crop production and food security. Plants have evolved several mechanisms to overcome challenging environments and respond to internal and external stimuli. One significant mechanism is the formation of biomolecular condensates driven by liquid–liquid phase separation. Biomolecular condensates have received much attention in the past decade, especially with regard to how plants perceive temperature fluctuations and their involvement in stress response and tolerance. In this review, we compile and discuss examples of plant biomolecular condensates regarding their composition, localization, and functions triggered by exposure to heat. Bioinformatic tools can be exploited to predict heat-induced biomolecular condensates. As the field of biomolecular condensates has emerged in the study of plants, many intriguing questions have arisen that have yet to be solved. Increased knowledge of biomolecular condensates will help in securing crop production and overcoming limitations caused by heat stress.

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ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules

Cited by 50 publications

References 59 publications

Monitoring mRNA Half-Life in Arabidopsis Using Droplet Digital PCR

Monitoring mRNA Half-Life in Arabidopsis Using Droplet Digital PCR

Phase separation-based visualization of protein-protein interactions and kinase activities in plants

Landscape of biomolecular condensates in heat stress responses

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