Albizia chinensis bark extract ameliorates the genotoxic effect of cyclophosphamide

Abstract

Background The genus Albizia (Leguminoseae) is used in folk medicine for the treatment of a wide range of ailments. Recently, saponins from plant origin have attracted much attention. Saponins are recorded to have a broad range of biological and pharmacological activities. This study was performed to evaluate the protective role of Albizia chinensis bark methanolic extract (MEAC) against the genotoxicity induced by cyclophosphamide (CP) using different mutagenic parameters. Results The results showed that MEAC induced an inhibitory effect against chromosomal aberrations of CP in mouse bone marrow and spermatocytes. Such effect was found to be significant (p < 0.01) with a dose of 100 mg/kg treated once for 24 h and also after repeated treatment at a dose of 25 mg/kg for 7 days. In sperm abnormalities, the protective effect of Albizia extract showed a dose-related relationship. Different doses of MEAC (25, 50, and 100 mg/kg) significantly (p < 0.01) ameliorated sperm abnormalities induced by CP dose-dependently. The percentage of sperm abnormalities was decreased to 5.14 ± 0.72 in the group of animals treated with CP plus MEAC (100 mg/kg) indicating an inhibitory effect of about 50%. Conclusion MEAC at the doses examined was non-genotoxic compared to control (negative) and exhibited a protective role against CP genotoxicity.

Histological Study of the Damaging Effect Induced by Cyclophosphamide on Intestinal Mucosa of Adult Male Albino Rat

“…Cyclophosphamide is an effective anticancer drug used in the management of many types of cancers, e.g., acute and chronic leukemia, lymphomas, multiple myeloma, breast cancer, ovarian carcinoma, neuroblastoma, and sarcoma (Nabil et al, 2020). It is also used as an immunesuppressant for the treatment of autoimmune diseases (Madondo et al, 2016).…”

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“…The medicinal plants of this species have been reported for hepatoprotective and antioxidant, acetylcholinesterase inhibitory 20, 21, anti-diabetic 22, ant-idiarrheal, antispasmodic, and Bronchodilator effects 23 . A. stipulata is a conventionally significant plant and is reported to possess enormous medicinal properties such as anti-ulcer, anti-proliferative, antioxidant, antidiabetic, anti-microbial and anti-inflammatory, spermicidal, thrombolytic, analgesic, anti-diarrheal, CNS depressant activities of various extract from different parts of the plant and its gum used as a polymer for control released tablets 24,34, which exposed that it has been contained terpenoids, steroids, flavonoids, phenols, saponins, inositol, and quinone. Moreover, previous phytochemical studies revealed the presence of several cytotoxic triterpenes, saponins, glycosides, flavonoids such as luteolin, quercetin, steroids namely Stigmasterol, β-Sitosterol, alkaloids, and phenols 26,35 .…”