Albumin-Encapsulated Liposomes: A Novel Drug Delivery Carrier With Hydrophobic Drugs Encapsulated in the Inner Aqueous Core

Okamoto, Yuko; Taguchi, Kazuaki; Yamasaki, Keishi; Saeedi, Mina; Kuroda, Shinya; Otagiri, Masaki

doi:10.1016/j.xphs.2017.08.003

Cited by 43 publications

(35 citation statements)

References 30 publications

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“…In addition, our previous study showed that BSA-liposomes possess good biocompatibility with nonhematologic toxicity and hepatic and renal injury associated with them. 8 Furthermore, on the basis of SAXS analyses, we estimate that the surfaces of BSA-liposomes are completely covered with PEG chains. As a result, the BSA-liposomes can avoid being captured and degraded by the mononuclear phagocyte system and acquire a stealth effect (the long blood retention).…”

Section: Discussionmentioning

confidence: 99%

“…As a result, the BSA-liposomes can avoid being captured and degraded by the mononuclear phagocyte system and acquire a stealth effect (the long blood retention). 8 These characteristics would be expected to confer on PTX–BSA-liposomes a high accumulation property at the tumor site via the EPR effect in AsPC-1 bearing mice. In fact, the in vivo accumulation of PTX–BSA-liposomes in the tumor site was observed starting 6 h after administration in AsPC-1 bearing mice (Figure 6a).…”

Section: Discussionmentioning

confidence: 99%

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Preparation, Characterization, and in Vitro/in Vivo Evaluation of Paclitaxel-Bound Albumin-Encapsulated Liposomes for the Treatment of Pancreatic Cancer

Okamoto¹,

Taguchi

Saeedi³

et al. 2019

ACS Omega

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Paclitaxel (PTX)-loaded liposomes were developed with the goal of enhancing the effects of cancer treatment. Although loading substances into the lipid membrane of liposome cause some destabilization of the lipid membrane, PTX was nearly exclusively embedded in the lipid membrane of liposomes, due to its low water solubility. Hydrophobic drugs can be encapsulated into the inner core of bovine serum albumin (BSA)-encapsulated liposomes (BSA-liposome) via noncovalent binding to albumin. Since PTX is able to noncovalently bind to albumin, we attempted to prepare PTX-loaded BSA-liposome (PTX–BSA-liposome). The amount of PTX loaded in the BSA-liposome could be increased substantially by using ethanol, since ethanol increases PTX solubility in BSA solutions via prompting the binding PTX to BSA. On the basis of the results of transmission electron microscopy and small-angle X-ray scattering, PTX–BSA-liposome formed unilamellar vesicles that were spherical in shape and the PTX was encapsulated into the inner aqueous core of the liposome as a form of PTX–BSA complex. In addition, the PTX–BSA-liposome, as well as nab-PTX, showed cytotoxicity against human pancreatic cancer cells, AsPC-1 cells, in a PTX concentration-dependent manner. The in vivo antitumor effect of PTX–BSA-liposomes was also observed in a mouse model that had been subcutaneously inoculated with pancreatic cancer cells by virtue of its high accumulation at the tumor site via the enhanced permeability retention effect. These results suggest that PTX–BSA-liposomes have the potential for serving as a novel PTX preparation method for the treatment of pancreatic cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Preparation, Characterization, and in Vitro/in Vivo Evaluation of Paclitaxel-Bound Albumin-Encapsulated Liposomes for the Treatment of Pancreatic Cancer

Okamoto¹,

Taguchi

Saeedi³

et al. 2019

ACS Omega

Self Cite

View full text Add to dashboard Cite

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“…[20,21] Phospoholipidic nanocarriers, such as liposomes, could also be used for delivery of hydrophobic drugs. [22][23][24] They could increase drug stability and enable drugs to pass through the cell membrane, as well as decrease the side effects and daily drug dosage intake. [25][26][27] Furthermore, the hydrogels with incorporated liposomes possess improved controlled drug releasing characteristics compared to hydrogels without liposomes.…”

Section: Introductionmentioning

confidence: 99%

Modification of hydrophilic polymer network to design a carrier for a poorly water‐soluble substance

Markovič

Panic

Šešlija

et al. 2020

Polymer Engineering & Sci

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pH sensitive, nontoxic, and biocompatible poly(methacrylic) acid (PMAA) based soft networks have been extensively used in the design of systems for targeted drug delivery. Still, their highly hydrophilic nature limits their potential to be used as a carrier of poorly water-soluble substances. With the aim to overcome this limitation, the present study details a new approach for modification of PMAA based carriers using two amphiphilic components: casein and liposomes. The FTIR analysis revealed structural features of each component as well as the synergetic effect that originated from the formation of specific interactions. Namely, hydrophobic interactions between the poorly watersoluble model drug (caffeine) and casein enabled caffeine encapsulation and controlled release, while addition of liposomes ensured better control of the release rate. The morphological properties of the carriers, swelling behavior, and release kinetics of caffeine were investigated depending on the variable synthesis parameters (neutralization degree of methacrylic acid, concentration of caffeine, presence/absence of liposomes) in two different media simulating the pH environment of human intestines and stomach. The data obtained from in vitro caffeine release were correlated and analyzed in detail using several mathematical models, indicating significant potential of investigated carriers for targeted delivery and controlled release of poorly water-soluble substances.

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“…2b) and interfere with specific DPTE-tbFGF mediated cellular uptake [57]. We did not investigate the fate of the liposome components after drug delivery, but expect them to be degraded and completely cleared from the cells [69].…”

Section: Discussionmentioning

confidence: 99%

Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery

et al. 2019

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Background The design of efficient drug delivery vectors requires versatile formulations able to simultaneously direct a multitude of molecular targets and to bypass the endosomal recycling pathway of cells. Liposomal-based vectors need the decoration of the lipid surface with specific peptides to fulfill the functional requirements. The unspecific binding of peptides to the lipid surface is often accompanied with uncontrolled formulations and thus preventing the molecular mechanisms of a successful therapy. Results We present a simple synthesis pathway to anchor cysteine-terminal peptides to thiol-reactive lipids for adequate and quantitative liposomal formulations. As a proof of concept, we have synthesized two different lipopeptides based on (a) the truncated Fibroblast Growth Factor (tbFGF) for cell targeting and (b) the pH sensitive and fusogenic GALA peptide for endosomal scape. Conclusions The incorporation of these two lipopeptides in the liposomal formulation improves the fibroblast cell targeting and promotes the direct delivery of cargo molecules to the cytoplasm of the cell. Electronic supplementary material The online version of this article (10.1186/s12951-019-0509-8) contains supplementary material, which is available to authorized users.

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Albumin-Encapsulated Liposomes: A Novel Drug Delivery Carrier With Hydrophobic Drugs Encapsulated in the Inner Aqueous Core

Cited by 43 publications

References 30 publications

Preparation, Characterization, and in Vitro/in Vivo Evaluation of Paclitaxel-Bound Albumin-Encapsulated Liposomes for the Treatment of Pancreatic Cancer

Preparation, Characterization, and in Vitro/in Vivo Evaluation of Paclitaxel-Bound Albumin-Encapsulated Liposomes for the Treatment of Pancreatic Cancer

Modification of hydrophilic polymer network to design a carrier for a poorly water‐soluble substance

Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery

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