Albumin-Enriched Fibrin Hydrogel Embedded in Active Ferromagnetic Networks Improves Osteoblast Differentiation and Vascular Self-Organisation

Levy, Galit Katarivas; Ong, John; Birch, Mark; Justin, Alexander W.; Markaki, Athina E.

doi:10.20944/preprints201909.0180.v1

Cited by 6 publications

(2 citation statements)

References 45 publications

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“…Khanna et al also prepared a heat-responsive BSA-based hydrogel, which was shown to be well suitable for topical drug delivery through a series of in vitro and in vivo experiments (Khanna et al, 2020). In addition, BSA-based hydrogels have also been investigated for applications in tissue regeneration, the preparation of thermosensitive or temperaturesensitive hydrogels, the prevention of post-operative adhesions and so on (Arabi et al, 2018;Katarivas Levy et al, 2019;Mao et al, 2021;Yoon et al, 2021).…”

Section: Bsa-based Hydrogelsmentioning

confidence: 99%

Research progress on albumin-based hydrogels: Properties, preparation methods, types and its application for antitumor-drug delivery and tissue engineering

Meng

Zhu

Deng

et al. 2023

Front. Bioeng. Biotechnol.

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Albumin is derived from blood plasma and is the most abundant protein in blood plasma, which has good mechanical properties, biocompatibility and degradability, so albumin is an ideal biomaterial for biomedical applications, and drug-carriers based on albumin can better reduce the cytotoxicity of drug. Currently, there are numerous reviews summarizing the research progress on drug-loaded albumin molecules or nanoparticles. In comparison, the study of albumin-based hydrogels is a relatively small area of research, and few articles have systematically summarized the research progress of albumin-based hydrogels, especially for drug delivery and tissue engineering. Thus, this review summarizes the functional features and preparation methods of albumin-based hydrogels, different types of albumin-based hydrogels and their applications in antitumor drugs, tissue regeneration engineering, etc. Also, potential directions for future research on albumin-based hydrogels are discussed.

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Section: Bsa-based Hydrogelsmentioning

confidence: 99%

Research progress on albumin-based hydrogels: Properties, preparation methods, types and its application for antitumor-drug delivery and tissue engineering

Meng

Zhu

Deng

et al. 2023

Front. Bioeng. Biotechnol.

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“…Recently, we demonstrated that human serum albumin (HSA) embedded in a fibrin hydrogel significantly promoted osteoblast differentiation and vascular self-organisation of human endothelial cells 5 . However, high cell numbers (10 6 cells/ml or greater) degrade these fibrin-based hydrogels within 8–10 days making it unsuitable for studying long term bone development and implantation into animal models.…”

Section: Introductionmentioning

confidence: 99%

Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment

Ong

Zhao

Levy

et al. 2020

Sci Rep

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Albumin-based hydrogels are increasingly attractive in tissue engineering because they provide a xeno-free, biocompatible and potentially patient-specific platform for tissue engineering and drug delivery. The majority of research on albumin hydrogels has focused on bovine serum albumin (BSA), leaving human serum albumin (HSA) comparatively understudied. Different gelation methods are usually employed for HSA and BSA, and variations in the amino acid sequences of HSA and BSA exist; these account for differences in the hydrogel properties. Heat-induced gelation of aqueous HSA is the easiest method of synthesizing HSA hydrogels however hydrogel opacity and poor cell attachment limit their usefulness in downstream applications. Here, a solution to this problem is presented. Stable and translucent HSA hydrogels were created by controlled thermal gelation and the addition of sodium chloride. the resulting bio-inert hydrogel was then subjected to air plasma treatment which functionalised its surface, enabling the attachment of basement membrane matrix (Geltrex). In vitro survival and proliferation studies of foetal human osteoblasts subsequently demonstrated good biocompatibility of functionalised albumin hydrogels compared to untreated samples. Thus, air plasma treatment enables functionalisation of inert heat-derived HSA hydrogels with extracellular matrix proteins and these may be used as a xeno-free platform for biomedical research or cell therapy. Albumin is an abundant non-glycosylated, 66.4 kDa protein in human serum that has a physiological half-life of approximately 19 days. It is synthesized predominantly by hepatocytes and poorly excreted through the renal glomerulus 1. Being poorly metabolised and weakly immunogenic, it is stable in vivo. Albumin is also versatile, acting as a weak pH buffer, and as a stabiliser to important proteins, hormones, metal ions, nanoparticles and drugs, making it an attractive biomaterial. These desirable attributes have led to extensive research into albumin as a protein conjugate for drug delivery and pharmacotherapy 1-3. However, the use of albumin-based hydrogels in biomedical research is comparatively under-studied 4. Recently, we demonstrated that human serum albumin (HSA) embedded in a fibrin hydrogel significantly promoted osteoblast differentiation and vascular self-organisation of human endothelial cells 5. However, high cell numbers (10 6 cells/ml or greater) degrade these fibrin-based hydrogels within 8-10 days making it unsuitable for studying long term bone development and implantation into animal models. Furthermore, it was anticipated that the implantation of fibrin-based scaffolds into bone would accelerate its degradation in vivo because of the presence of serum fibrinolytics and movement of bone during locomotion. Therefore, albumin-based hydrogels were explored as an alternative material for scaffolds. Heat-derived bovine serum albumin (BSA) hydrogels (20% w/v) exhibit high Young's modulus (~ 55 kPa) and longer degradation periods (~ 4 to 8 weeks) in vitro and...

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Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells

et al. 2021

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In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.

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Albumin-Enriched Fibrin Hydrogel Embedded in Active Ferromagnetic Networks Improves Osteoblast Differentiation and Vascular Self-Organisation

Cited by 6 publications

References 45 publications

Research progress on albumin-based hydrogels: Properties, preparation methods, types and its application for antitumor-drug delivery and tissue engineering

Research progress on albumin-based hydrogels: Properties, preparation methods, types and its application for antitumor-drug delivery and tissue engineering

Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment

Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells

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