Albumin–genipin solder for laser tissue repair

Lauto, Antonio; Foster, L. John R.; Ferris, L. A.; Avolio, Alberto; Zwaneveld, N.; Poole-Warren, Laura A.

doi:10.1002/lsm.20081

Cited by 35 publications

(32 citation statements)

References 30 publications

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“…For the initial improvement of tensile strength and a consequent limitation of thermal damage the use of albumin solders is very beneficial [19]. In this study, we used BSA for soldering.…”

Section: Discussionmentioning

confidence: 99%

“…To increase initial tensile strength of the wound solders have successfully been applied improving laser-mediated tissue welding through denaturation and bond formation [18]. Albumin as a solder for laser tissue fusion has been studied and employed in different clinical and experimental settings [19]. Exsudating wounds show abundant albumin concentrations, which might even redundantize exogenous albumin application.…”

Section: Introductionmentioning

confidence: 99%

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Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

et al. 2010

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Background and Objective: Collagen scaffolds are popular for the reconstitution of dermal equivalents. Usually, these scaffolds are fixed with sutures or staples and in many cases these devices have to be removed in a second procedure. Laser-mediated tissue welding in a wet environment is a potential alternative for collagen scaffold fixation and may be advantageous to suture, staple, and tissue glue fixation. Materials and Methods: Welding was performed with a continuous-wave diode laser system emitting radiation at a wavelength of 968 nm. Tensile strength after fixation to porcine skin and laser parameters were determined in vitro. In vivo, 24 excisional deep partial thickness wounds were created on flanks of two Goettingen mini pigs and covered with collagen scaffolds. These were randomized and fixated with either (1) staples, (2) fibrin glue, or (3) laser-mediated welding. Tissue biopsies for histological analysis were periodically performed and analyzed for wound healing progression, epidermal thickness, and extracellular matrix formation. Results: Biomechanical stability after laser welding was time dependent. A dwell time of up to 10 seconds led to a strong bonding with a tensile strength of more than 30 g. In vivo, the wound healing process was macroscopically comparable in all groups and showed no significant differences. Microscopic analysis determined a more progressed and quicker wound closure in both the laser and staples group compared to the fibrin glue fixated scaffold. Laser-mediated fixation led to a significantly reduced epidermal thickness when compared with stapling or fibrin glue (P < 0.05). Conclusions: Laser tissue welding is a feasible approach for temporary fixation of collagen scaffolds to the wound bed. It improves wound healing properties and may lead to faster wound healing and cosmetically better scarring. Laser tissue welding is thus a very interesting and promising alternative to currently established fixation methods in a single step, no touch procedure

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“…For the initial improvement of tensile strength and a consequent limitation of thermal damage the use of albumin solders is very beneficial [19]. In this study, we used BSA for soldering.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

et al. 2010

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“…The numbers in brackets indicate the reference from which the data were obtained. of PLGA ssLAVR was improved with the addition of the protein cross-linking agent genipin [107], which enhances cohesive and adhesive bonding and thereby increases the stability of the weld ( Figure 11C). With the addition of genipin, 80% of the end-to-end sutureless PLGA ssLAVAed carotid arteries withstood a 24-h ex vivo hemodynamic test without bursting or leaking (Figure 12) [108].…”

Section: Laser-scaffold Interactionsmentioning

confidence: 99%

“…With an optimal combination of chromophore concentration and lasing parameters, denaturation that starts at the solder base, as observed with solders containing high albumin content, potentially produces stronger adhesive bonding than the superficial denaturation seen with low albumin content solders. Furthermore, adhesive bonding can be increased by the addition of a protein cross-linking agent such as genipin, which chemically cross-links albumin to tissue collagens [107,111].…”

Section: Improvements In Adhesive Bondingmentioning

confidence: 99%

“…The other lasing parameters, i.e., irradiance, irradiation time, and irradiation mode, should be determined empirically. A low-irradiance continuous-wave laser operated in an intermittent regime is beneficial for uniform heat distribution and the limitation of thermal damage [86], whereas ultrashort irradiation with pulsed lasers provides better control of heat deposition and more efficient heat transfer [107].…”

Section: Future Perspectivesmentioning

confidence: 99%

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Laser-assisted vessel welding: state of the art and future outlook

Pabittei

Boon

Heger

et al. 2015

JCTRes

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Laser-assisted vascular welding (LAVW) is an experimental technique being developed as an alternative to suture anastomosis. In comparison to mechanical anastomosis, LAVW is less traumatic, non-immunogenic, provides immediate water tight sealant, and possibly a faster and easier procedure for minimally invasive surgery. This review focuses on technical advances to improve welding strength and to reduce thermal damage in LAVW. In terms of welding strength, LAVW has evolved from the photothermally-induced microvascular anastomosis, requiring stay sutures to support welding strength, to sutureless anastomoses of medium-sized vessels, withstanding physiological and supraphysiological pressure. Further improvements in anastomotic strength could be achieved by the use of chromophore-containing albumin solder and the employment of (biocompatible) polymeric scaffolds. The anastomotic strength and the stability of welds achieved with such a modality, referred to as scaffold-and solder-enhanced LAVW (ssLAVW), are dependent on the intermolecular bonding of solder molecules (cohesive bonding) and the bonding between solder and tissue collagen (adhesive bonding). Presently, the challenges of ssLAVW include (1) obtaining an optimal balance between cohesive and adhesive bonding and (2) minimizing thermal damage. The modulation of thermodynamics during welding, the application of semi-solid solder, and the use of a scaffold that supports both cohesive and adhesive strength are essential to improve welding strength and to limit thermal damage.

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Construction of a Band‐Aid Like Cardiac Patch for Myocardial Infarction with Controllable H₂S Release

Chen

Pan

et al. 2022

Advanced Science

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Excessive or persistent inflammation incites cardiomyocytes necrosis by generating reactive oxygen species in myocardial infarction (MI). Hydrogen sulfide (H2S), a gaseous signal molecule, can quickly permeate cells and tissues, growing concerned for its cardioprotective effects. However, short resident time and strong side effects greatly restrict its application. Herein, a complex scaffold (AAB) is first developed to slowly release H2S for myocardial protection by integrating alginate modified with 2‐aminopyridine‐5‐thiocarboxamide (H2S donor) into albumin electrospun fibers. Next, a band‐aid like patch is constructed based on AAB (center) and nanocomposite scaffold which comprises albumin scaffold and black phosphorus nanosheets (BPNSs). With near‐infrared laser (808 nm), thermal energy generated by BPNSs can locally change the molecular structure of fibrous scaffold, thereby attaching patch to the myocardium. In this study, it is also demonstrated that AAB can enhance regenerative M2 macrophage and attenuate inflammatory polarization of macrophages via reduction in intracellular ROS. Eventually, this engineered cardiac patch can relieve inflammation and promote angiogenesis after MI, and thereby recover heart function, providing a promising therapeutic strategy for MI treatment.

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Albumin–genipin solder for laser tissue repair

Abstract: Addition of a chemical crosslinking agent, such as genipin, significantly increased the tensile strength of adhesive-tissue bonds. A proposed mechanism for this enhanced bond strength is the synergistic action of mechanical adhesion with chemical crosslinking by genipin.

Cited by 35 publications

References 30 publications

Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

Laser-assisted vessel welding: state of the art and future outlook

Construction of a Band‐Aid Like Cardiac Patch for Myocardial Infarction with Controllable H₂S Release

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Albumin–genipin solder for laser tissue repair

Abstract: Addition of a chemical crosslinking agent, such as genipin, significantly increased the tensile strength of adhesive-tissue bonds. A proposed mechanism for this enhanced bond strength is the synergistic action of mechanical adhesion with chemical crosslinking by genipin.

Cited by 35 publications

References 30 publications

Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

Laser-assisted vessel welding: state of the art and future outlook

Construction of a Band‐Aid Like Cardiac Patch for Myocardial Infarction with Controllable H2S Release

Contact Info

Product

Resources

About

Construction of a Band‐Aid Like Cardiac Patch for Myocardial Infarction with Controllable H₂S Release