Albumina glicata. Un indice di controllo glicemico da rivalutare

Festa, Roberto; Mosca, Andrea; Lapolla, Annunziata; Paleari, Renata; Foti, Daniela; Ferrai, Grazia; Testa, Roberto

doi:10.1007/s13631-012-0045-0

Cited by 3 publications

(1 citation statement)

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“…Now there is the need to further explore the clinical value of the test in different patient subsets. Determination of reference intervals in a selected population according to American Diabetes Association (ADA) criteria is next to be investigated to pair Caucasian population values with those previously published (32)(33)(34)(35). Furthermore, GA% vs HbA 1c comparisons will be investigated.…”

Section: Discussionmentioning

confidence: 99%

Analytical Performances of an Enzymatic Assay for the Measurement of Glycated Albumin

Testa

Guerra

Bonfigli

et al. 2016

The Journal of Applied Laboratory Medicine

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Background: Short to intermediate integrated glycemic control is best determined by glycated albumin (GA). This assay is appropriate when interpretation of glycated hemoglobin (HbA 1c) is critical because of hemoglobinopathies, severe anemias, or other factors that affect red blood lifespan as hemodialysis. We evaluated a new assay based on the enzymatic quantification of GA by ketoamine oxidase and an albumin-specific protease. Methods: Limits of blank, detection, and quantification; precision; linearity; accuracy; interferences; correlation with HbA 1c ; and serum vs plasma study have been evaluated on ILab ® systems. Results: Limit of blank, detection, and quantification for GA (g/L) were, respectively, 0.26, 0.36, and 1.15. Repeatability and within-device precision CVs were lower than 2.11%, 1.61%, and 1.56% for GA (g/L), albumin (g/L), and GA%, respectively. Linearity for GA (g/L) and GA% was 1.2-36.8 and 5.5-92.2, respectively. Highest deviation from linearity was <11% and recovery was higher than 90%. Accuracy against the certified ReCCS Japan Clinical Chemistry Reference Material (JCCRM) 611 was <1%. Classical interfering substances had no significant impact. Correlation of GA% between ILab ® Taurus and ADVIA system was y = 1.02[GA%]+0.25; R 2 = 0.994. No difference was found in the determination of GA% in serum vs plasma. Conclusions: GA enzymatic assay is a reliable, fully automated method allowing accurate and precise determination of GA in a routine laboratory. IMPACT STATEMENT This article will benefit both diabetic patients with hemoglobinopathies or reduced red blood cell lifespan that need an alternative marker for glycemic control and people at risk of developing diabetes. Data show glycated albumin (GA) assay reliability. Moreover, it is easy to use, being a clinical chemistry assay: it can be run on standard clinical chemistry automated systems. This article summarizes almost a full proof of performance report of a GA assay investigating most of the performance aspects as precision, accuracy, linearity, and interference study.

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Section: Discussionmentioning

confidence: 99%