Alcanivorax nanhaiticus sp. nov., isolated from deep sea sediment

Abstract

A taxonomic study was carried out on strain 19-m-6 T , which was isolated from deep sea sediment of the South China Sea during the screening of alkane-degrading bacteria. The isolate was Gram-reaction-negative, and oxidase-and catalase-positive. On the basis of 16S rRNA gene sequence similarity, strain 19-m-6 T was shown to belong to the genus Alcanivorax, related to Alcanivorax jadensis T9 T (97.5 %), Alcanivorax hongdengensis A-11-3 T (97.3 %), A. lcanivorax borkumensis SK2 T (96.6 %) and seven other species of the genus Alcanivorax (93.9-95.4 %). Average nucleotide identity values between strain 19-m-6 T and A. jadensis T9 T , A. hongdengensis A-11-3 T and A. borkumensis SK2 T were 85.12, 85.87 and 84.35 %, respectively. The estimated DNA-DNA hybridization values between strain 19-m-6 T and these three type strains were 22.0, 22.6 and 21.2 %, respectively. Four alkane hydroxylase (alkB) genes were obtained from the draft genome sequence. The G+C content of the chromosomal DNA was 56.44 mol%. The major fatty acids were C 16 : 0 , C 18 : 1 !7c and summed feature 3 (C 16 : 1 !6c and/or C 16 : 1 !7c). The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, one aminolipid, three phospholipids, two glycolipids and two aminophospholipids. According to its phenotypic features, fatty acid composition and 16S rRNA gene sequence, the novel strain fitted well into the genus Alcanivorax, but could be clearly distinguished from all other known Alcanivorax species described to date. The name Alcanivorax nanhaiticus sp. nov. is thus proposed, with 19-m-6 T (=MCCC 1A05629 T =KCTC 52137 T ) as the type strain.

Effects of Elevated pCO2 on the Survival and Growth of Portunus trituberculatus

“…These bacteria are residents in the marine environment and some have distinctive ecological functions. For instance, the genus Alcanivorax is well known for its ability to degrade n-alkanes (Wu et al, 2009;Lai et al, 2016;Konieczna et al, 2018). The genus Oceanicola is capable of degrading polycyclic aromatic hydrocarbons (Yuan et al, 2009;Gutierrez et al, 2017).…”

Antibiofilm activity of secondary metabolites of sponge-associated bacterium Alcanivorax sp. from the Red Sea

“…The bacteria Alcanivorax is widely distributed in marine environments occupying different depths and habitats as free-living or in association with other marine organisms. It is found on the surface water and surface sediments, deep seawater, and deep-sea sediments (Lai et al, 2013;Barbato et al, 2015;Lai et al, 2016;Karthick and Mohanraju, 2018;Song et al, 2018;Liu J. et al, 2019;Liao et al, 2020). It has been reported to be living in a symbiotic relationship with several organisms in the marine environment such as seaweeds and microalgae (Karthick and Mohanraju, 2018;Chernikova et al, 2020).…”

Comparison of Hydrocarbon-Degrading Consortia from Surface and Deep Waters of the Eastern Mediterranean Sea: Characterization and Degradation Potential

“…In the Deep consortium incubations, Alcanivorax and Vibrio dominated at 14 • C but were replaced by the cosmopolitan Pseudoalteromonas and Pseudomonas at 25 • C after Day 6. The role of Alcanivorax species in deep-sea oil-spill remediation is ambiguous; several species of Alcanivorax have been isolated from the deep sea [52][53][54] yet Alcanivorax was not reported among the dominant taxa in the deep hydrocarbon plume during DWH and, based on ex situ experiments, this genus is not considered as characteristic of the microbial community response to oil contamination in the deep sea [11,45,55,56]. Nevertheless, Alcanivoraceae were identified as key players in crude oil degradation in deep waters of the EMS [20], in agreement with the results of this study.…”