Alcian Blue 8GS: A New Stain for Mucin

Steedman, Hilary

doi:10.1242/jcs.s3-91.16.477

Cited by 166 publications

(34 citation statements)

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“…The epidermal and subepidermal (/lands and their relation to feeding These secretions can be divided into mucoid, giving a positive reaction with P.A.S. and Alcian Blue (Steedman, 1950), and the non-mucoid or rhabdoid, giving a negative reaction.…”

Section: The Structure Of the Gut And Course Of Digestionmentioning

confidence: 99%

Studies on Feeding, Digestion, and Food Storage in Free-Living Flatworms (Platyhelminthes: Turbellaria)

Jennings¹

1957

The Biological Bulletin

165

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Investigations upon turbellarian nutrition (summarized by Hyman, 1951 andYonge, 1954) have so far dealt mainly with triclads, where intracellular digestion has been demonstrated but the possibility of some supplementary intraluminar digestion not fully explored. Triclad nutrition has therefore been re-examined, using Polycelis cornuta, and the opportunity taken to make comparable investigations on representatives of the other turbellarian orders. In each case the nature of the food and the feeding mechanism, the structure of the gut and the course of digestion, and the nature and location of the food reserves have been studied. The various mucoid and rhabdoid secretions have also been examined and an assessment made

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Section: The Structure Of the Gut And Course Of Digestionmentioning

confidence: 99%

Studies on Feeding, Digestion, and Food Storage in Free-Living Flatworms (Platyhelminthes: Turbellaria)

Jennings¹

1957

The Biological Bulletin

165

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“…The sections were stained with 1% Alcian Blue in distilled water for 10–40 s and rinsed with distilled water to remove excess stain. After the tissue was stained with Haemalum technique, it was counterstained with eosin, dehydrated and was mounted in resinous medium (Steedman, 1950).…”

Section: Methodsmentioning

confidence: 99%

Incidence of multiple bacterial infections in Pacific whiteleg shrimp, Litopenaeus vannamei

Dewangan

Gopalakrishnan

Shankar³

et al. 2022

Aquaculture Research

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A rare incidence of multiple bacterial infections was reported in Litopenaeus vannamei grow‐out ponds in India. The pathogenic organisms identified are Aeromonas veronii, Bacillus flexus, Kurthia gibsonii, Shewanella decolorationis, Bacillus cereus and Shewanella amazonensis. The infected shrimp showed various clinical and morphological changes such as mucus secretion in the hepatopancreas, bacterial spots in the carapace, red or pink discoloration of abdomen with black stripes and highly melanized body. The Histological studies of the hepatopancreas revealed bacterial plaque in the lumen, inflammatory response, necrosis of connective tissue and lipid droplets. The histology of stomach detected degradation of spongy connective tissue, hypertrophied nucleus and the abdomen showed bacterial plaques in the gaps of the muscle fibre. Histochemically, both gram‐positive and gram‐negative bacteria's were identified in the hepatopancreas and the presence of mucus in the lumen of hepatopancreatic microtubules was confirmed by Alcian blue staining. The electron microscopic studies of the infected shrimp showed multiple bacterial infections and cellular responses like phagocytosis in the hepatopancreas, using transmission electron microscope and scanning electron microscopic confirmed the presence of bacillus and coccobacillus‐shaped bacteria in carapace.

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“…In addition to these, and to certain special methods described in the literature, the following methods are referred to in this paper : a. Alcian blue 8GS. 4 This stain, whose use for mucus has recently been suggested by Steedman (1950), was employed in 1% aqueous solution; metanil yellow was used as a counterstain.…”

Section: Methodsmentioning

confidence: 99%

Cytological Studies on Mucus Formation and Secretion in Busycon

Ronkin¹

1952

The Biological Bulletin

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Alcian Blue 8GS: A New Stain for Mucin

Abstract: A new method of staining mucin is described. The stain used is alcian blue 8GS The method is rapid and easy in application. The results are clear and permanent.

Cited by 166 publications

References 0 publications

Studies on Feeding, Digestion, and Food Storage in Free-Living Flatworms (Platyhelminthes: Turbellaria)

Studies on Feeding, Digestion, and Food Storage in Free-Living Flatworms (Platyhelminthes: Turbellaria)

Incidence of multiple bacterial infections in Pacific whiteleg shrimp, Litopenaeus vannamei

Cytological Studies on Mucus Formation and Secretion in Busycon

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