Alcian blue staining intestinal goblet cell antigen (GOA): A Marker for gastric signet ring cell and colonic colloidal carcinoma

Rapp, W.; Wurster, K

doi:10.1007/bf01476863

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…Then, samples of liver, duodenum, jejunum and ileum were collected and fixed in Davidson’s solution (100 mL glacial acetic acid, 300 mL 95% ethyl alcohol, 200 mL 10% neutral buffered formalin and 300 mL distilled water) for at least 24 h. Then, samples were dehydrated, infiltrated and embedded in paraffin following common histological routine. Blocks were cut in 5 μm sections and stained with hematoxylin and eosin with Alcian Blue for goblet cells staining ( Rapp and Wurster, 1978 ).…”

Section: Methodsmentioning

confidence: 99%

Applying I see inside histological methodology to evaluate gut health in broilers challenged with Eimeria

Belote

Soares

Tujimoto-Silva

et al. 2019

Veterinary Parasitology

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Section: Methodsmentioning

confidence: 99%

Applying I see inside histological methodology to evaluate gut health in broilers challenged with Eimeria

Belote

Soares

Tujimoto-Silva

et al. 2019

Veterinary Parasitology

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“…For the histological analysis, samples of the proximal ileum from two birds per pen (n = 80) were collected and fixed in Davidson's solution (100 mL glacial acetic acid, 300 mL 95% ethyl alcohol, 200 mL 10% neutral buffered formalin and 300 mL distilled water) for at least 24 h. Then, samples were dehydrated, infiltrated and embedded in paraffin following common histological routine. Blocks were cut in 5 µm sections and stained with hematoxylin and eosin plus Alcian Blue (23). An average of 20 intestinal villi per slide was evaluated per bird in 10× objective (using 20× and 40× objective to confirm alterations) of an optical microscope (Nikon Eclipse E200, São Paulo, SP, Brazil).…”

Section: Experimental Design Diets and Treatmentsmentioning

confidence: 99%

Protected Blend of Organic Acids and Essential Oils Improves Growth Performance, Nutrient Digestibility, and Intestinal Health of Broiler Chickens Undergoing an Intestinal Challenge

et al. 2020

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The growing restriction of antibiotic growth promoters (AGP) use in farming animals has raised a concern regarding the viability of the animal production system. In this new context, feed additives with proven positive impact on intestinal health may be used as strategy to avoid losses on performance. The aim of this study was to evaluate the effects of a protected blend of organic acids and essential oils [P(OA+EO)] on growth performance, nutrient digestibility, and intestinal health of broiler chickens. A total of 1,080 Cobb × Cobb 500 male broilers were randomly distributed in four treatments with 10 replicates (27 birds/each). Treatments were as follow: non-challenged control; challenged control; AGP (enramycin at 10 g/t); and P(OA+EO) at 300 g/t. All birds on challenged groups were challenged with Eimeria spp. at 1 day and with Clostridium perfringens at 11, 12, and 13 days. Body weight gain (BWG), feed intake and feed conversion ratio (FCR) were evaluated until 42 days. At 17 days, one bird per pen was orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and blood samples were collected for FITC-d detection to assess intestinal permeability. At 21 days, apparent ileal nutrient and energy (IDE) digestibility, intestinal macroscopic and histologic alterations (ISI) and, expression of mucin2 (MUC2), claudin1 (CLDN1), and occludin (OCLN) genes in the jejunum were evaluated. From 1 to 42 days, birds from the non-challenged and P(OA+EO) groups had greater (P < 0.001) BWG compared to challenged control and AGP groups. The challenged control group presented the worst FCR (P < 0.001). IDE was 106 kcal/kg greater when broilers were fed P(OA+EO) compared to the challenged control group. Broilers supplemented with P(OA+EO) had improved intestinal integrity with lower blood FITC-d concentration and ISI scores, and greater expression of MUC2, CLDN1, and OCLN genes compared to the challenged control group (P < 0.05). In conclusion, the P(OA+EO) and the AGP led to increased growth performance, nutrient digestibility and intestinal health of challenged broilers. A marked difference occurred in favor of the P(OA+EO), suggesting that this blend may be used to improve intestinal health and broiler growth performance in AGP free programs.

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“…Five infected chickens from each group were sacrificed with sterile knives and a strict aseptic technique, and then small tissue specimens from the intestines in all groups were collected in vials containing 10% formalin. Histopathology sections were prepared using the formalin sample and hematoxylin and eosin (H&E) were used to stain all histological sections (Rapp and Wurster, 1978).…”

Section: Histopathological Examinationmentioning

confidence: 99%

Efficacy of administration of ampicillin and/or dimetridazole in the management of experimentally induced necrotic enteritis in broiler chickens.

Youssif,

Salem,

salah

et al. 2023

Benha Veterinary Medical Journal

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This study aimed to investigate the efficacy of oral administration of ampicillin and dimetridazole (DMZ) either alone or in combination for the treatment of necrotic enteritis (NE) caused by Clostridium perfringens (CP) infection in broiler chickens. One hundred Hubbard chicks were allocated into five groups (A, B, C, D, and E, 20 birds each). Birds in all groups (except those in group A) were orally inoculated at the age of 15 days with 0.5 mL of CP broth culture (10 9 CFU/mL). Two days later, medications were orally given once daily for five days as follows; Group A and B were left untreated. Group C and D were treated with a therapeutic dose of ampicillin (20 mg/ kg b.wt) and DMZ (20 mg/ kg b.wt) respectively, while group E was treated with both ampicillin and DMZ. Clinical symptoms, weight gain, feed conversion rate (FCR), and the score of intestinal lesions were used to estimate the effectiveness of the investigated drugs. The results showed that administration of ampicillin and DMZ combination was followed by noticeable increases in body weight, weight gain, FCR, red blood cell count (RBCs), and hemoglobin concentration associated with a significant decrease in leukocyte. In addition, intestinal lesions in the ampicillin and DMZ groups showed nearly normal intestinal villi and enterocytes indicating recovery. The efficiency of ampicillin or DMZ was varied, but the combination of both drugs was greater. In conclusion, using ampicillin and DMZ together rather than separately was significantly more effective in controlling NE in broiler chickens.

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Alcian blue staining intestinal goblet cell antigen (GOA): A Marker for gastric signet ring cell and colonic colloidal carcinoma

Cited by 7 publications

References 16 publications

Applying I see inside histological methodology to evaluate gut health in broilers challenged with Eimeria

Applying I see inside histological methodology to evaluate gut health in broilers challenged with Eimeria

Protected Blend of Organic Acids and Essential Oils Improves Growth Performance, Nutrient Digestibility, and Intestinal Health of Broiler Chickens Undergoing an Intestinal Challenge

Efficacy of administration of ampicillin and/or dimetridazole in the management of experimentally induced necrotic enteritis in broiler chickens.

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