Alcian Stains for Histochemical Localization of Acid and Sulfated Polysaccharides in Algae

Parker, Barry; Diboll, Alfred G.

doi:10.2216/i0031-8884-6-1-37.1

Cited by 129 publications

(42 citation statements)

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“…3) also secrete highly sulphated adhesives with small carboxyl components. The results with Grammatophora differ from those of other species since only the centres of the adhesive links stain with the PAS reaction, while the protein specific Chayen et al (1973) McCully (1966) Chayen et al (1973) Pearse (1968) Blanquet (1976) Parker & Diboll (1966 Scott & Dorling (1965) Pearse (1968) Heath (1961) Hollander (1964) McGee-Russell (1955 ninhydrin-Schiff procedure produces the reverse effect. This is almost certainly due in part to a coating of bacteria and claymineral particulates, but the positive localization of acidic groups in the adhesive may indicate a predominance of these in the outer regions of the pad.…”

Section: Pad-forming Speciesmentioning

confidence: 90%

Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms

Daniel

Chamberlain

Jones

1987

British Phycological Journal

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Section: Pad-forming Speciesmentioning

confidence: 90%

Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms

Daniel

Chamberlain

Jones

1987

British Phycological Journal

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“…Alcian blue (AB) has been used in a number of studies for quantification of AP and SP in algae (e.g., Parker and Diboll 1966;Ramus 1977;Crayton 1982). In the staining reaction process, a pH below 1.0 in the dye solution allows AB to be used for quantification and characterization of SP (Crayton 1982).…”

Section: Discussionmentioning

confidence: 99%

Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)

Xing

et al. 2008

J Appl Phycol

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There are several apparent developmental stages in the life cycle of Nostoc sphaeroides Kützing, an edible cyanobacterium found mainly in paddy fields in central China. The cytochemical changes in developmental stages such as hormogonia, aseriate stage, filamentous stage and colony in N. sphaeroides were examined using fluorescent staining and colorimetric methods. The staining of acidic and sulfated polysaccharides increased with development when hormogonia were used as the starting point. Acidic polysaccharides (AP) were most abundant at the aseriate stage and then decreased, while the sulfated polysaccharides (SP) were highest at the colony stage. Quantitatively, along the developmental process from hormogonia to colony, total carbohydrates first increased, then became stable, and then reached their highest level at the colony stage, while reducing sugars were highest at the hormogonia stage and then decreased sharply once development began. SP were not detectable in the hot water soluble polysaccharides (HWSP), and hormogonia had the lowest content of AP, while old colonies had the highest. The AP content of the aseriate stage, filamentous stage and young colony stage were very similar. The evolutionary relationships reflected in the developmental stages of N. sphaeroides are discussed.

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“…Es ya conocida la importancia qu e tienen los «tubos cribosos» I o f¡lamento s c ribosos p rimedu lares en la trans locación de s u~t a n ci as el lo largo del tal o adulto de Macro cysris (Parkcr, 1965;1966 ;Shih el al., 1983). Las células que los componen (element os cribosos) tienen pl acas cflbosas con poro s entre 0,45 f-lm y 4 ,50 f-lm de diámetro , según las especies, la edad de la planta y la ubi ca ción anatómica (Parker & Huber, 1965 ;Schmitz, 198 1;Hay, 1986 ;Núñez & Alveal , 1988) También se señala la presencla de otros filamentos en la médul a, de posición más in terna , los llamado s fi lame ntos trompe ta (S mith , 1939) o filamentos c ribosos medulares (Esau, 1969).…”

Section: Discusionunclassified

“…N T ejada y M . Go nzalez tra ns locación d e fotosintato s (O liver, 1887;Sykes, 1908;Parker & Diboll, 1966;Parker, 197 1; BarraJes e l al., J981;Grenvill e el al., 1982;úñez & Alveal, 1988). Sykes ( 1908) realizó un detallado estudio del crecimiento y la di fe ren ciac ió n de talos adultos de M. pyriJera basánd ose en observ ac iones sobre plantas macroscó picas .…”

Section: Introduccionunclassified

Ultraestructura de esporofitos jovenes de Macrocystis pyrifera (Linnaeus) C. Agardh (Phaeophyceae, Laminariales)

1998

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RESUMENSe investigó el desarrollo anatómico de esporofitos jóvenes de Macrocystis pyrifera obtenidos a partir de cigotos en cultivos de laboratorio. Los talos eran monostromáticos luego de dos semanas de creci miento y las células eran similares entre sí, fotosintéticas y conectadas por campos de puntuación primarios. A las cinco semanas, los talos eran poliestromáticos y estaban diferenciados internamente en meristodermo, corteza y médula. Filamentos cribosos longitudinales aparecieron en la médula, de sarrollándose de la corteza interna y rodeados por una matriz intercelular. Cada elemento criboso pre sentaba un núcleo, cloroplastos y varios plasmodesmos, los cuales estaban distribuidos más o menos al azar en paredes transversales o agrupados en campos de puntuación primarios en paredes longitudinales. Luego de nueve semanas, los talos eran más gruesos y estaban diferenciados morfológicamente en una lámina, estípite y grampón rizoidal. Además de los filamentos cribosos, apa recieron filamentos menores o «hifas» en la médula. Se discute el desarrollo del esporofito joven y la relación de nuestras observaciones con estudios en otras algas pardas. ABSTRACTThe anatomical development of young sporophytes of Macrocystis pyrifera obtained from zygotes in laboratory cultures was investigated. Thalli were monostromatic alter two weeks of growth , and cells were similar, photosynthetic, and connected by primary pit fields. Thalli were polystromatic and diHerentiated internally into meristoderm, cortex and medulla in the fifth week . Longitudinal sieve filaments appeared in the medulla developing from the inner cortex and they were surrounded by an intercellular matrix. Individual sieve elements contained a nucleus, chloroplasts and several plasmodesmata, which were more or less randomly distributed in transverse walls or they were grouped in primary pit fields in longitudinal walls. Thalli were thicker and diHerentiated morphologically into a single lamina, stipe and rhizoidal holdfast alter nine weeks . In addition to sieve filaments, smaller lilaments or «hyphae» appeared in the medulla. Possible developmental pathways to adult stages and the relationship 01 our finding with other studies lor brown algae are discussed.

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Alcian Stains for Histochemical Localization of Acid and Sulfated Polysaccharides in Algae

Cited by 129 publications

References 0 publications

Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms

Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms

Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)

Ultraestructura de esporofitos jovenes de Macrocystis pyrifera (Linnaeus) C. Agardh (Phaeophyceae, Laminariales)

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