Alcohol and nicotine co-Administration during pregnancy and lactation periods alters sensory discrimination of adult NMRI mice offspring

Sabzalizadeh, Mansoureh; Afarinesh, Mohammad Reza; Mafi, Fatemeh; Mosanejad, Elahe; Haghpanah, Tahereh; Golshan, Fatemeh; Koohkan, Faezeh; Ezzatabadipour, Massood; Sheibani, Vahid

doi:10.1016/j.physbeh.2019.112731

Cited by 13 publications

(12 citation statements)

References 77 publications

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“…Unlikely, some have reported a synergistic effect 92 . In contrast to the current findings, Sabzalizadeh et al 19 revealed that these two substances could synergistically impair cognition in pups. However, it is yet unknown whether such a different effect is dependent on the developing tissue; the interaction of these substances during intrauterine programming might differently affect cells in a developing reproductive system relative to the nervous system.…”

Section: Discussioncontrasting

confidence: 99%

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Genetic and epigenetic modifications of F1 offspring’s sperm cells following in utero and lactational combined exposure to nicotine and ethanol

Pabarja

Hakemi

Musanejad

et al. 2021

Sci Rep

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It is well established that maternal lifestyle during pregnancy and lactation affects the intrauterine programming of F1 offspring. However, despite the co-use of alcohol and nicotine is a common habit, the effects of exposure to both substances on the reproductive system of F1 male offspring and the underlying mechanisms of developmental programming have not been investigated. The present study aimed to examine pre- and postnatal concurrent exposure to these substances on genetic and epigenetic alterations of sperm cells as well as testis properties of F1 offspring compared with exposure to each substance alone. Pregnant dams in the F0 generation randomly received normal saline, nicotine, ethanol, and combinations throughout full gestation and lactation periods. Sperm cells and testes of F1 male offspring were collected at postnatal day 90 for further experiments. High levels of sperm DNA fragmentation were observed in all exposed offspring. Regarding epigenetic alterations, there was a significant increase in the relative transcript abundance of histone deacetylase 1 and 2 in all exposed sperm cells. Moreover, despite a decrease in the expression level of DNA methyltransferase (DNMT) 3A, no marked differences were found in the expression levels of DNMT1 and 3B in any of the exposed sperm cells compared to non-exposed ones. Interestingly, combined exposure had less prominent effects relative to exposure to each substance alone. The changes in the testicular and sperm parameters were compatible with genetic and epigenetic alterations. However, MDA level as an oxidative stress indicator increased in all exposed pups, which may be responsible for such outputs. In conclusion, maternal co-exposure to these substances exhibited epigenotoxicity effects on germline cells of F1 male offspring, although these effects were less marked relative to exposure to each substance alone. These counteracting effects may be explained by cross-tolerance and probably less impairment of the antioxidant defense system.

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Section: Discussioncontrasting

confidence: 99%

“… 80 . Nicotine + Ethanol group (Nic + Ethn): the pregnant mice daily received the same doses of nicotine and ethanol, simultaneously 19 .…”

Section: Methodsmentioning

confidence: 99%

Genetic and epigenetic modifications of F1 offspring’s sperm cells following in utero and lactational combined exposure to nicotine and ethanol

Pabarja

Hakemi

Musanejad

et al. 2021

Sci Rep

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“…Yet, some studies determined that this counteracting effect was only observed in acute exposure dose 66 and in contrast, some reported a synergistically contribution of these drugs 67 in impairment even in acute dose exposure. In contrast to our ndings, Sabzalizadeh and co-workers 68 revealed that these two substances could synergistically impair cognition in pups. However, it is yet unknown that whether such a different effect is dependent on the developing tissue; so that the interaction of these substances during embryofoetal life might differently affect cells in developing reproductive system rather than the nervous system.…”

Section: Discussioncontrasting

confidence: 99%

“…Nicotine + Ethanol group (Nic+Ethn): the pregnant mice daily received the same doses of nicotine and ethanol, simultaneously 68 .…”

Section: Injectionmentioning

confidence: 99%

In Utero and Lactational Exposure of F1 Adult Pups to Ethanol and Nicotine: Alteration of The Reproductive System and Epigenetic Modifying Enzymes of Sperm Cells?

Pabarja¹,

Hakemi²,

Musanejad³

et al. 2021

Preprint

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Co-use of alcohol and nicotine is a common habit which could affect reproductive system. The present study was designed to investigate the effect of in-utero and lactational exposure of offspring to alcohol, nicotine, and combinations on the reproductive system and epigenetic alterations of male gamete. Pregnant mice and lactating NMRI pups randomly received nicotine, ethanol, and combinations during gestational days 1 until weaning. Sperm and testes were collected on postnatal day 90 for further experiments. Exposure to these substances, particularly, nicotine, highly affected testicular Johnsen’s score, sperm parameters including; number, motility, viability, DNA integrity and also serum MDA level. Interestingly, concurrent exposure to nicotine and alcohol somehow reversed the effects. Quantitative real-time PCR data showed an increase in the mRNA level of histone deacetylation 1 and 2 and a decrease in the level of DNA methyltransferase (DNMT) 3A, while no marked differences in the expression level of DNMT1 and 3B were observed between the exposed and non-exposed pups. Alleviating effects were observed in the epigenetic modifying enzymes transcripts of co-exposed pups compared with the exposure to nicotine or alcohol. Taken together, our findings determined that exposure of offspring in-utero and during lactational period to these substances could result in lasting epigenetic changes in sperm cells. However, unexpectedly co-exposure of pups to nicotine and alcohol lessened these negative effects.

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“…Both drugs disrupt the development of the nervous system, producing long-lasting neurological and behavioral outcomes in the offspring [16][17][18] . These include increased risk of attention deficit hyperactivity disorder [19][20][21] , learning and memory deficits [22][23][24] and impaired sensory processing [25][26][27] .…”

Section: Introductionmentioning

confidence: 99%

Sex- and age-dependent differences in nicotine susceptibility evoked by developmental exposure to tobacco smoke and/or ethanol in mice

Nunes‐Freitas

Manhães

Dutra-Tavares

et al. 2020

J Dev Orig Health Dis

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Either tobacco smoking or alcohol consumption during pregnancy sex-selectively increases susceptibility to drugs of abuse later in life. Considering that pregnant smoking women are frequently intermittent consumers of alcoholic beverages, here, we investigated whether a short-term ethanol exposure restricted to the brain growth spurt period when combined with chronic developmental exposure to tobacco smoke aggravates susceptibility to nicotine in adolescent and adult mice. Swiss male and female mice were exposed to tobacco smoke (SMK; research cigarettes 3R4F, whole-body exposure, 8 h/daily) or ambient air during the gestational period and until the tenth postnatal day (PN). Ethanol (ETOH, 2 g/Kg, 25%, i.p.) or saline was injected in the pups every other day from PN2 to PN10. There were no significant differences in cotinine (nicotine metabolite) and ethanol serum levels among SMK, ETOH and SMK + ETOH groups. During adolescence (PN30) and adulthood (PN90), nicotine (NIC, 0.5 mg/Kg) susceptibility was evaluated in the conditioned place preference and open field tests. NIC impact was more evident in females: SMK, ETOH and SMK + ETOH adolescent females were equally more susceptible to nicotine-induced place preference than control animals. At adulthood, SMK and SMK + ETOH adult females exhibited a nicotine-evoked hyperlocomotor profile in the open field, with a stronger effect in the SMK + ETOH group. Our results indicate that ethanol exposure during the brain growth spurt, when combined to developmental exposure to tobacco smoke, increases nicotine susceptibility with stronger effects in adult females. This result represents a worsened outcome from the early developmental dual exposure and may predispose nicotine use/abuse later in life.

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Alcohol and nicotine co-Administration during pregnancy and lactation periods alters sensory discrimination of adult NMRI mice offspring

Cited by 13 publications

References 77 publications

Genetic and epigenetic modifications of F1 offspring’s sperm cells following in utero and lactational combined exposure to nicotine and ethanol

Genetic and epigenetic modifications of F1 offspring’s sperm cells following in utero and lactational combined exposure to nicotine and ethanol

In Utero and Lactational Exposure of F1 Adult Pups to Ethanol and Nicotine: Alteration of The Reproductive System and Epigenetic Modifying Enzymes of Sperm Cells?

Sex- and age-dependent differences in nicotine susceptibility evoked by developmental exposure to tobacco smoke and/or ethanol in mice

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