Alcohol decreases intestinal ratio of <i>Lactobacillus</i> to <i>Enterobacteriaceae</i> and induces hepatic immune tolerance in a murine model of DSS-colitis

Kuprys, Paulius V.; Cannon, Abigail R.; Shieh, Jennifer; Iftekhar, Noama; Park, Sun K; Ding, Xianzhong; Choudhry, Mashkoor A.

doi:10.1080/19490976.2020.1838236

Cited by 23 publications

(22 citation statements)

References 86 publications

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“…It has been proved that Bifidobacterium promotes the conversion of primary bile acids into secondary bile acids [ 32 , 33 ]. Besides, Lactobacillus , Caldicoprobacter are the main genus associated with lactic acid production and reducing pH in the gut, which promotes the production of short-chain fatty acids (SCFAs) by bacteria and maintains intestinal health [ 34 , 35 ]. The result was consistent with the previous report that ethanol has been shown to increase fecal pH and disrupt gut microbiota homeostasis [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Besides, Lactobacillus , Caldicoprobacter are the main genus associated with lactic acid production and reducing pH in the gut, which promotes the production of short-chain fatty acids (SCFAs) by bacteria and maintains intestinal health [ 34 , 35 ]. The result was consistent with the previous report that ethanol has been shown to increase fecal pH and disrupt gut microbiota homeostasis [ 34 ]. Moreover, Lactobacillus and Bifidobacterium encode ADH and ALDH to mediate the metabolism of ethanol and acetaldehyde, respectively, reducing the accumulation of acetaldehyde in the intestinal tract, which can improve the intestinal barrier function, showing a protective action on the development of alcohol-induced liver injury in mice [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

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The Protective Effects of Ganoderic Acids from Ganoderma lucidum Fruiting Body on Alcoholic Liver Injury and Intestinal Microflora Disturbance in Mice with Excessive Alcohol Intake

Cao

Huang

You

et al. 2022

Foods

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This study aimed to investigate the protective effects of ganoderic acids (GA) from Ganoderma lucidum against liver injury and intestinal microbial disorder in mice with excessive alcohol intake. Results showed GA supplement significantly inhibited the abnormal elevation of the liver index, serum lipid parameters, aspartate aminotransferase and alanine aminotransferase in mice exposed to alcohol intake, and also significantly protected the excessive lipid accumulation and pathological changes. Alcohol-induced oxidative stress in the liver was significantly ameliorated by GA intervention through reducing the levels of maleic dialdehyde and lactate dehydrogenase and increasing the levels of glutathione, catalase, superoxide dismutase and alcohol dehydrogenase. Intestinal microbiota profiling demonstrated GA intervention modulated the composition of intestinal microflora by increasing the levels of Lactobacillus, Faecalibaculum, Romboutsia, Bifidobacterium and decreasing the Helicobacter level. Furthermore, liver metabolomic profiling suggested GA intervention had a remarkable regulatory effect on liver metabolism with excessive alcohol consumption. Moreover, GA intervention regulated mRNA levels of alcohol metabolism, fatty lipid metabolism, oxidative stress, bile acid biosynthesis and metabolism-related genes in the liver. Conclusively, these findings demonstrate GA intervention can significantly relieve alcoholic liver injury and it is hopeful to become a new functional food ingredient for the prevention of alcoholic liver injury.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Protective Effects of Ganoderic Acids from Ganoderma lucidum Fruiting Body on Alcoholic Liver Injury and Intestinal Microflora Disturbance in Mice with Excessive Alcohol Intake

Cao

Huang

You

et al. 2022

Foods

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“…2E). Furthermore, ethanol exposure markedly increased the abundance of Enterbacteriaceae, which are closely associated with intestinal diseases (33), and this was significantly suppressed by the treatments of LA, VC and LA+VC (P<0.01; Fig. 2E).…”

Section: La Plus Vc Treatment Restores the Gut Microbiota Homeostasismentioning

confidence: 93%

Lactobacillus acidophilus and vitamin C attenuate ethanol‑induced intestinal and liver injury in mice

Xing

Wang

2021

Exp Ther Med

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Ethanol exposure frequently induces intestinal and liver injury, dysbiosis of the gut microbiota and vitamin C (VC) deficiency. Gut microbiota-targeted therapy is emerging as an important adjuvant method for protecting the body against ethanol-induced injury, particularly probiotics containing Lactobacillus acidophilus (LA). However, the feasibility and efficiency of using synbiotics containing LA and VC against ethanol-induced injury remained largely undetermined. To examine the advantages of LA+VC, their effect was evaluated in an ethanol-fed mouse model. The results suggested that LA+VC restored gut microbiota homeostasis and reinstated the immune balance of colonic T-regulatory cells (CD4 + CD45 + forkhead box p3 + ). In addition, intestinal barrier disorders were improved via upregulating tight junction proteins (claudin-2, zona occludens-1 and occludin) and mucus secretion, which prevented the translocation of lipopolysaccharide into circulatory systems and subsequently reduced the expression of Toll-like receptor 4 in liver tissues. In this context, LA+VC treatment reduced the inflammatory response in the liver, which was likely responsible for the improved liver function in ethanol-challenged mice. Collectively, these results indicated that LA+VC treatment significantly protected the intestine and liver from ethanol damage by enhancing intestinal barrier function and reducing systemic inflammation.The present study paved the way for further exploration of synbiotics based on Lactobacillus species and VC.

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“…Goblet cells were observed with Alcian blue/periodic acid–Schiff (AB/PAS) straining. These sections were observed under a microscope (BX53M; Olympus, Tokyo, Japan) and were scored based on a four-point scale (0–4) upon examination for exudates, polymorphonuclear leukocyte invasions, epithelial damage, submucosal edema, and necrosis ( Kuprys et al, 2020 ; Ma et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…The remaining colon samples were stored in liquid nitrogen until further use. scored based on a four-point scale (0-4) upon examination for exudates, polymorphonuclear leukocyte invasions, epithelial damage, submucosal edema, and necrosis (Kuprys et al, 2020;Ma et al, 2020).…”

Section: Experimental Setup To Induce Uc In Micementioning

confidence: 99%

Transcriptome and Gut Microbiota Profiling Revealed the Protective Effect of Tibetan Tea on Ulcerative Colitis in Mice

Wang

et al. 2022

Front. Microbiol.

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Traditionally, Ya’an Tibetan tea is routinely consumed by local people in the Tibet region. It is believed to possess promising anti-inflammatory benefits. This study was conducted to elucidate the protective impact of Tibetan tea extract (TTE) on dextran sodium sulfate (DSS)-induced colitis in mice. Mice were split into four groups: control (C) group, Tibetan tea (T) group, DSS-induced model (CD) group, and Tibetan tea + DSS (TD) group. The intake of TTE significantly reduced the clinical symptoms of ulcerative colitis (UC) by alleviating the impact of cellular damage and reducing glandular hypertrophy and the infiltration of inflammatory cells. UC led to a prominent shift of the microbial communities in the gut. Interestingly, the beneficial microbes, such as Lactobacillus reuteri, Bifidobacterium choerinum, and Lactobacillus intestinalis, were significantly increased in TTE-treated mice when compared to any other experimental group. The transcriptome analysis revealed that the positive effect of TTE on UC could be attributed to changes in the G alpha (i) signaling pathway and the innate immune system. The genes related to inflammation and immune system pathways were differentially expressed in the TTE-treated group. Moreover, the relative expression of genes linked to the inflammatory TLR4/MyD88/NF-κB signaling pathway was significantly downregulated toward the level of normal control samples in the TD group. Overall, this study revealed the modulatory effect by which TTE reversed the development and severity of chronic colon damage.

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Alcohol decreases intestinal ratio of Lactobacillus to Enterobacteriaceae and induces hepatic immune tolerance in a murine model of DSS-colitis

Cited by 23 publications

References 86 publications

The Protective Effects of Ganoderic Acids from Ganoderma lucidum Fruiting Body on Alcoholic Liver Injury and Intestinal Microflora Disturbance in Mice with Excessive Alcohol Intake

The Protective Effects of Ganoderic Acids from Ganoderma lucidum Fruiting Body on Alcoholic Liver Injury and Intestinal Microflora Disturbance in Mice with Excessive Alcohol Intake

Lactobacillus acidophilus and vitamin C attenuate ethanol‑induced intestinal and liver injury in mice

Transcriptome and Gut Microbiota Profiling Revealed the Protective Effect of Tibetan Tea on Ulcerative Colitis in Mice

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