Alcohol Dehydrogenase in the Human Colon and Rectum

Seitz, Helmut K.; Egerer, Gerlinde; Oneta, Carl; Kramer, S; Sieg, Andreas; Klee, Fritz; Simanowski, Ulrich A.

doi:10.1159/000201322

Cited by 39 publications

(26 citation statements)

References 9 publications

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“…In contrast, the serum activity of other tested classes of ADH isoenzymes in patients with cancer does not significantly differ between heavy drinkers and moderate drinkers. Class I ADH isoenzymes are expressed mainly in the liver, but they are also present in the other organs of the gastrointestinal tract [25,26]. The elevated activity of class I isoenzymes in our study was apparently caused by the enzyme released from the organ damaged by ethanol.…”

Section: Discussionmentioning

confidence: 44%

Alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in the sera of patients with esophageal cancer

et al. 2009

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Various alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) exist in the human esophageal mucosa. In our last experiments we have shown that ADH and ALDH are present also in the esophageal cancer cells. Moreover, the activities of total ADH and class IV isoenzymes were significantly higher in cancer tissue than in healthy mucosa, which suggests that these changes may be reflected by enzyme activity in the serum. Therefore, we measured the activity of total alcohol dehydrogenase, and classes I-IV of this enzyme and aldehyde dehydrogenase in the sera of patients with this cancer. Serum samples were taken for routine biochemical investigation from 67 patients with esophageal cancer before treatment. Total ADH activity was measured by photometric method with p-nitrosodimethylaniline (NDMA) as a substrate and ALDH activity by the fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I and II isoenzymes, we employed the fluorometric methods, with class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was measured by the photometric method with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. A statistically significant increase of class IV alcohol dehydrogenase isoenzymes was found in the sera of cancer patients. The median activity of this class isoenzyme in the total cancer group increased by about 26.5% (7.42 mU/l) in comparison to the control level (5.46 mU/l). The total alcohol dehydrogenase activity was significantly higher (30%) among patients with cancer. The activities of other tested ADH isoenzymes and total ALDH were unchanged. The activity of the class I ADH isoenzyme was significantly higher in the sera of drinkers with esophageal cancer than non-drinking patients. The increased total activity of alcohol dehydrogenase and class IV isoenzyme in the sera of patients with esophageal cancer probably can be caused by release of this isoenzyme from cancer cells or might be stimulated by alcohol drinking.

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Section: Discussionmentioning

confidence: 44%

Alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in the sera of patients with esophageal cancer

et al. 2009

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“…Therefore, it is very important to find markers that would detect a malignant cell transformation as early as possible. Human colorectal mucosa is equipped with alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) which perform many important physiological functions [2]. ADH exists in multiple molecular forms which have been grouped into six classes.…”

Section: Introductionmentioning

confidence: 99%

The Diagnostic Value of Alcohol Dehydrogenase (ADH) Isoenzymes and Aldehyde Dehydrogenase (ALDH) Measurement in the Sera of Colorectal Cancer Patients

2010

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“…Human colorectal mucosa contains isoenzymes of three classes of alcohol dehydrogenase (ADH): I, III and IV [1]. Several aldehyde dehydrogenase (ALDH) isoenzymes are also expressed in colorectal mucosa but the activity of this enzyme is much lower than ADH [2,3].…”

Section: Introductionmentioning

confidence: 99%

Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the sera of patients with colorectal cancer

et al. 2007

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Various isoenzymes of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) exist in human colorectal mucosa. In our last experiments we have shown that ADH and ALDH are present also in colorectal cancer cells. Moreover the activities of total ADH and class I isoenzymes were significantly higher in cancer tissue than healthy mucosa. This may suggest that these changes may be reflected by enzyme activity in the serum. Therefore, we have measured the activity of total ADH, and classes I-IV of this enzyme and ALDH in the sera of patients suffering from this cancer. Total ADH activity was measured by a photometric method with p-nitrosodimethylaniline (NDMA) as a substrate and ALDH activity by the fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I and II isoenzymes we employed fluorometric methods, with class-specific fluorogenic substrates. The activity of class III ADH was measured by the photometric method with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. Serum samples were taken for routine biochemical investigations from 52 patients with colorectal carcinoma before treatment. A statistically significant increase of class I ADH isoenzymes was found. Therefore the total ADH activity was also significantly increased. The total ALDH and the activity of other tested ADH isoenzymes were unchanged. We also observed the increasing tendency of ADH I activity in accordance with the advance of disease. The activity of class I ADH isoenzymes was elevated in the serum of patients with colorectal cancer. This activity was derived from colorectal cancer cells and probably from severely damaged liver by metastatic disease.

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Alcohol Dehydrogenase in the Human Colon and Rectum

Cited by 39 publications

References 9 publications

Alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in the sera of patients with esophageal cancer

Alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in the sera of patients with esophageal cancer

The Diagnostic Value of Alcohol Dehydrogenase (ADH) Isoenzymes and Aldehyde Dehydrogenase (ALDH) Measurement in the Sera of Colorectal Cancer Patients

Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the sera of patients with colorectal cancer

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