Alcohol dehydrogenase zymogram, its inheritance and anaerobic germinability of seeds of Echinochloa weeds.

Yamasue, Yuji; Tanisaka, Takatoshi; Kusanagi, Tokuichi

doi:10.1270/jsbbs1951.40.53

Cited by 6 publications

(10 citation statements)

References 12 publications

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“…The A 1 and A 5 zymograms had never been previously observed in seeds of naturally occurring Echinochloa, nor among the progeny of hybrids between formosensis and praticola. The A 1 A 3 A 5 zymogram had been previously found in seeds of crus-galli at a very low frequency (Yamasue et al, 1990).…”

Section: Resultsmentioning

confidence: 77%

“…To induce ADH mutations, one hundred seeds of formosensis were irradiated with 400 Gy (RD 50 for seedling height) of 60 Co gamma-ray at 10 3 Gy/ h. In the M 1 generation, plants derived from the irradiated seeds were self-pollinated by bagging and harvested as panicle progeny. Twelve seeds from a panicle of each fertile M 1 plant were tested by polyacrylamide gel electrophoresis described elsewhere (Yamasue et al, 1990). Because most of the ADH activity within seeds is localized in the embryo (Yamasue et al, 1990), whole seeds were used to produce ADH zymo-grams.…”

Section: Methodsmentioning

confidence: 99%

“…Twelve seeds from a panicle of each fertile M 1 plant were tested by polyacrylamide gel electrophoresis described elsewhere (Yamasue et al, 1990). Because most of the ADH activity within seeds is localized in the embryo (Yamasue et al, 1990), whole seeds were used to produce ADH zymo-grams. Many of the M 1 plants showed variation among progeny seeds within a panicle for ADH zymograms and were discarded.…”

Section: Methodsmentioning

confidence: 99%

“…Seeds of these weeds markedly differ in alcohol dehydrogenase (ADH: E.C.1.1.1.1) zymograms variously composed of five bands: A 1 , A 2 , A 3 , A 4 , and A 5 . The band specified by A 1 displays the most rapid migration toward the anode and the band specified by A 5 the slowest (Yamasue et al, 1990). Formosensis, with a few exceptions, shows the 5-banded phenotype A 1 A 2 A 3 A 4 A 5 , and praticola has only the A 3 ADH band.…”

Section: Introductionmentioning

confidence: 99%

“…Crus-galli occupies a widespread distribution and is highly polymorphic for ADH. From an earlier hybridization experiment between formosensis and praticola, Yamasue et al (1990) suggested that ADH zymograms in E. crus-galli seeds were controlled by two structural genes. However, we could infrequently find crus-galli with the 3-banded phenotype A 1 A 3 A 5 in natural fields and it could not be explained on the basis of two structural genes.…”

Section: Introductionmentioning

confidence: 99%

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The inheritance of alcohol dehydrogenase isozymes in seeds of hexaploid Echinochloa crus-galli Beauv.

et al. 1998

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To clarify the mode of inheritance of alcohol dehydrogenase (ADH) isozymes in Echinochloa crus-galli, we induced single-banded mutant lines for ADH by gammaray irradiation; the irradiation of the 5-banded E. crus-galli var. formosensis (phenotype A 1 A 2 A 3 A 4 A 5 ) produced two single-banded mutant lines, A 1 and A 5 . All pairwise reciprocal crosses were made among the single-banded mutant lines and the naturally occurring single-banded type E. crus-galli var. praticola that displays only band A 3 . The ADH zymograms from the resulting F 1 progenies were identified by polyacrylamide gel electrophoresis and the relative staining densities of the bands were determined by densitometer scanning for several important E. crus-galli phenotypes and F 1 individuals. All F 1 seeds displayed both parental bands plus an additional band located intermediate to the parental bands. Moreover, the ratios of the band densities were in agreement with those expected from the kinetics of ADH dimer formation. The results can be accounted for by three loci encoding three ADH subunits. Also, the data indicate that the A 3 band of the 5-banded phenotype consists of two different dimeric products, homo-and hetero-dimers.

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Section: Resultsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The inheritance of alcohol dehydrogenase isozymes in seeds of hexaploid Echinochloa crus-galli Beauv.

et al. 1998

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Weed Management

Bayer¹

1991

Rice

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Construction of a comparative RFLP map of Echinochloa crus-galli toward QTL analysis of flooding tolerance

et al. 2003

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To analyze quantitative trait loci (QTLs) affecting flooding tolerance and other physiological and morphological traits in Echinochloa crus-galli, a restriction fragment length polymorphism (RFLP) map was constructed using 55 plants of the F(2) population ( E. crus-galli var. praticola x E. crus-galli var. formosensis). One hundred forty-one loci formed 41 linkage groups. The total map size was 1,468 cM and the average size of linkage groups was 35.8 cM. The average distance between markers was 14.7 cM and the range was 0-37.2 cM. Early comparisons to the genetic maps of other taxa suggest appreciable synteny with buffelgrass ( Pennisetum spp.) and sorghum ( Sorghum spp.). One hundred ninty-one F(2) plants were used to analyze QTLs of flooding tolerance, plant morphology, heading date, number of leaves, and plant height. For flooding tolerance, two QTLs were detected and one was mapped on linkage group 24. Other traits, including plant morphology, heading date, number of leaves, and plant height were highly correlated. Three genomic regions accounted for most of the mapped QTLs, each explaining 2-4 of the significant marker-trait associations. The high observed correlation between the traits appears to result from QTLs with a large contribution to the phenotypic variance at the same or nearby locations.

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Alcohol dehydrogenase zymogram, its inheritance and anaerobic germinability of seeds of Echinochloa weeds.

Cited by 6 publications

References 12 publications

The inheritance of alcohol dehydrogenase isozymes in seeds of hexaploid Echinochloa crus-galli Beauv.

The inheritance of alcohol dehydrogenase isozymes in seeds of hexaploid Echinochloa crus-galli Beauv.

Weed Management

Construction of a comparative RFLP map of Echinochloa crus-galli toward QTL analysis of flooding tolerance

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