Alcohol ethoxylates mediate their bacteriostatic effect by altering the cell membrane of Escherichia coli NCTC 8196

Moore, Suzanne L; Denyer, Stephen Paul; Hanlon, G. W.; Olliff, Cedric J.; Lansley, Alison B.; Rabone, K.; Jones, Martin V.

doi:10.1016/j.ijantimicag.2006.08.023

Cited by 12 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well known that nucleotides such as DNA and RNA are crucial factors responsible for cell repair and replication [28]. The release of nucleotides and their derived compounds including DNA and RNA from bacteria can be quantified by monitoring their absorbance at 260 nm because these substances possess strong UV absorption at this wavelength [29,30]. In our present study, asiatic acid treatments effectively promoted nucleotides release from the intracellular compartments of test bacteria, which was reflected in their increased absorbance at 260 nm.…”

Section: Discussionmentioning

confidence: 52%

Antibacterial effects and action modes of asiatic acid

Liu¹,

Liu²,

Mong³

2015

BioMed

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 52%

Antibacterial effects and action modes of asiatic acid

Liu¹,

Liu²,

Mong³

2015

BioMed

View full text Add to dashboard Cite

“…Alcohol ethoxylates are a major class of nonionic surfactants which are often used in household detergents, cleaners and personal care products and have previously shown bacteriostatic effects due to their direct impact on the bacterial cell membrane leading to the leakage of cytoplasmic components, indicating an increase in membrane permeability (48). An increase in membrane permeability would allow microbicides to more readily transverse the cytoplasmic membrane increasing their access to intracellular target sites.…”

Section: Discussionmentioning

confidence: 99%

Effects of Formulation on Microbicide Potency and Mitigation of the Development of Bacterial Insusceptibility

Cowley

Forbes

Amézquita

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

bRisk assessments of the potential for microbicides to select for reduced bacterial susceptibility have been based largely on data generated through the exposure of bacteria to microbicides in aqueous solution. Since microbicides are normally formulated with multiple excipients, we have investigated the effect of formulation on antimicrobial activity and the induction of bacterial insusceptibility. We tested 8 species of bacteria (7 genera) before and after repeated exposure (14 passages), using a previously validated gradient plating system, for their susceptibilities to the microbicides benzalkonium chloride, benzisothiozolinone, chlorhexidine, didecyldimethyl ammonium chloride, DMDM-hydantoin, polyhexamethylene biguanide, thymol, and triclosan in aqueous solution (nonformulated) and in formulation with excipients often deployed in consumer products. Susceptibilities were also assessed following an additional 14 passages without microbicide to determine the stability of any susceptibility changes. MICs and minimum bactericidal concentrations (MBC) were on average 11-fold lower for formulated microbicides than for nonformulated microbicides. After exposure to the antimicrobial compounds, of 72 combinations of microbicide and bacterium there were 19 >4-fold (mean, 8-fold) increases in MIC for nonformulated and 8 >4-fold (mean, 2-fold) increases in MIC for formulated microbicides. Furthermore, there were 20 >4-fold increases in MBC (mean, 8-fold) for nonformulated and 10 >4-fold (mean, 2-fold) increases in MBC for formulated microbicides. Susceptibility decreases fully or partially reverted back to preexposure values for 49% of MICs and 72% of MBCs after further passage. In summary, formulated microbicides exhibited greater antibacterial potency than unformulated actives and susceptibility decreases after repeated exposure were lower in frequency and extent. Microbicides are broad-spectrum chemical agents that inactivate microorganisms (1-3). They are widely deployed throughout health care (4-6), domestic (7,8), and industrial (9-11) environments, where their application includes antisepsis (12), hard surface disinfection (13), and pharmaceutical product preservation (14). They may also be incorporated into medical device coatings, for instance in sutures (15), wound dressings (16), and urinary catheters (17) to inhibit bacterial adhesion and subsequent biofilm formation.It has been hypothesized that the use of microbicides could select for bacterial adaptation, resulting in reduced efficacy of the primary agent, as well as potentially decreasing bacterial susceptibility to chemically unrelated agents such as other microbicides and antibiotics (18). While there have been reports documenting the laboratory selection of bacteria with decreased microbicide sensitivity following repeated exposure to microbicides in highly selective conditions, it remains unclear whether this commonly occurs in the environment (19-24).The majority of studies reporting reductions in microbicide susceptibility have used the active compo...

show abstract

“…Indeed, at low concentrations, the mechanism involves the penetration of the detergent molecules into the lipid membrane leading to increase its fluidity. In contrast, at higher concentrations, the extraction of membrane constituents is ensured by micellar solubilization [84,85].…”

Section: I) Complexation Of Lipids and Consequencesmentioning

confidence: 99%

Interactions between cyclodextrins and cellular components: Towards greener medical applications?

Leclercq¹

2016

Beilstein J. Org. Chem.

View full text Add to dashboard Cite

In the field of host–guest chemistry, some of the most widely used hosts are probably cyclodextrins (CDs). As CDs are able to increase the water solubility of numerous drugs by inclusion into their hydrophobic cavity, they have been widespread used to develop numerous pharmaceutical formulations. Nevertheless, CDs are also able to interact with endogenous substances that originate from an organism, tissue or cell. These interactions can be useful for a vast array of topics including cholesterol manipulation, treatment of Alzheimer’s disease, control of pathogens, etc. In addition, the use of natural CDs offers the great advantage of avoiding or reducing the use of common petroleum-sourced drugs. In this paper, the general features and applications of CDs have been reviewed as well as their interactions with isolated biomolecules leading to the formation of inclusion or exclusion complexes. Finally, some potential medical applications are highlighted throughout several examples.

show abstract

Alcohol ethoxylates mediate their bacteriostatic effect by altering the cell membrane of Escherichia coli NCTC 8196

Cited by 12 publications

References 49 publications

Antibacterial effects and action modes of asiatic acid

Antibacterial effects and action modes of asiatic acid

Effects of Formulation on Microbicide Potency and Mitigation of the Development of Bacterial Insusceptibility

Interactions between cyclodextrins and cellular components: Towards greener medical applications?

Contact Info

Product

Resources

About