Alcohol, Gestation and Breastfeeding: Selenium as an Antioxidant Therapy

Ojeda, María Luisa; Nogales, Fátima; Vázquez, Beatriz Sierra; Delgado, Ma José; Murillo, María Luisa Ojeda; Carreras, Olimpia

doi:10.1093/alcalc/agp004

Cited by 42 publications

(64 citation statements)

References 30 publications

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“…The source of radiation was a Se electrodeless discharge lamp. The instrumental operating conditions and the reagents are the same as those we used in the previous paper by Ojeda et al (2009). Samples: serum samples were diluted fivefold in 0.2% v/v HNO 3 and 0.2% Triton X-100 solutions and urine samples were diluted 1:2 v/v.…”

Section: Se Analysismentioning

confidence: 99%

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Nogales¹,

Ojeda²,

Fenutría³

et al. 2013

Reproduction

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Selenium (Se), an essential trace metal, is important in both growth and reproduction and is the constituent of different selenoproteins. The glutathione peroxidase (GPx) family is the most studied as it prevents oxidative stress. Liver oxidation is considered as another mechanism involved in low birth weight. Therefore, in order to ascertain whether GPx is related to the effects of Se on growth during gestation and lactation, three groups of rat pups were used: control, Se deficient (SD), and Se supplemented (SS). Morphological parameters and reproductive indices were evaluated. Hepatic Se levels were measured by graphite furnace atomic absorption while spectrophotometry was used for activity of antioxidant enzymes and oxidative stress markers in liver and western blotting for expression of hepatic GPx1 and GPx4. The SD diet increased mortality at birth; decreased viability and survival indices; and stunted growth, length, and liver development in offspring, thus decreasing hepatic Se levels, GPx, glutathione reductase, and catalase activities, while increasing superoxide dismutase activity and protein oxidation. The SS diet counteracted all the above results. GPx1 expression was heavily regulated by Se dietary intake; however, although Se dietary deficiency reduced GPx4 expression, this decrease was not as pronounced. Therefore, it can be concluded that Se dietary intake is intimately related to growth, length, and directly regulating GPx activity primarily via GPx1 and secondly to GPx4, thus affecting liver oxidation and development. These results suggest that if risk of uterine growth retardation is suspected, or if a neonate with low birth weight presents with signs of liver oxidation, it may be beneficial to know about Se status.

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Section: Se Analysismentioning

confidence: 99%

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Nogales¹,

Ojeda²,

Fenutría³

et al. 2013

Reproduction

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“…A non-signifi cant decrease in the GPx activity indicated a lowered demand for the conversion of peroxides to water as a result of the changes in the SOD activity. In contrast to retinoic acid treatment, in ethanol-treated rats, the GPx activity increased signifi cantly in the offspring (Ojeda et al, 2009). It is well known that GPx inactivation and SOD downregulation cause an increase in oxidative stress (Li et al, 2000).…”

Section: Discussionmentioning

confidence: 95%

Postnatal Development of Spinal Cord and Liver Antioxidant Status in the Young of Retinol-Overdosed Female Rats

Patlevič

Vašková

Vaško

et al. 2013

Zeitschrift Für Naturforschung C

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The metabolic form of vitamin A, retinol, has a pivotal role in the nervous system development and neuronal differentiation, both during embryogenesis through maternal-fetal support and in the early postnatal life. Retinoic acid was administered orally at a dose of 10 mg/kg body weight to pregnant female rats through days 8 -10 of gestation. Spinal cord sections were processed for histochemical visualization one day after birth and on day 21, when weaning is expected. NADPH-diaphorase (NADPH-d)-positive neurons were found in the dorsal horn, around the central canal, and at the intermediolateral cell column on postnatal days 1 and 21 in both control and experimental groups. There were no NADPHd-positive structures in the ventral horn. The results suggest that prenatal administration of high doses of retinoic acid is not associated with postnatal morphological changes in NADPH-d-positive neurons in the rat spinal cord. Levels of antioxidants and related enzymes in retinoid storage organs were measured to estimate possible side effects. The activities of enzymes detoxifying superoxide radicals and peroxides were supressed after birth. A decrease in the level of reduced glutathione was observed on postnatal day 21, indicating an unbalanced redox environment.

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“…According to what we have previously reported [8], the effects of ethanol exposure during gestation and lactation on folic acid intestinal absorption showed that intestinal folate absorption appears to be upregulated in suckling rats. Our research group has also found, in pups exposed to ethanol and supplemented with folic acid during the gestation and lactation periods, an increase in intestinal longitude (control, alcohol and alcohol plus folic acid: 53.56 ± 1.49, 43.7 ± 0.92 and 48.36 ± 1.69 cm, respectively) and intestinal mucosa weight (control, alcohol and alcohol plus folic acid: 15 ± 0.8, 12 ± 0.8 and 15 ± 1 mg/cm 2 , respectively).We have recently described that ethanol decreases selenium (Se) retention in dams, affecting their gestational parameters and the body weight, and the antioxidant balance of their progeny [9][10][11][12]. This essential trace element is of fundamental importance to health due to the antioxidant, anti-inflammatory and chemopreventive properties attributed to its presence in at least 25 selenoproteins such as glutathione peroxidase (GPx) [13].…”

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confidence: 99%

“…57, No. 6, 2011 SELENOMETHIONINE ABSORPTION IN DUODENUM OF ETHANOL-EXPOSED RAT OFFSRPING 709 their intestinal development; moreover, our research group has found that Se or Se plus folic acid supplementation during gestation and lactation could prevent liver oxidative damage in the pups, thus improving their development and health [10,11].At present, a limited number of studies have explored Se uptake in offspring rats [16] or infants [17,18], and there are no reports concerning this in ethanol-exposed pups. In this context, Anan et al (2009) [19] found that suckling pups mainly obtained Se presenting in breast-milk in organic form as Se-Met.…”

mentioning

confidence: 99%

“…The source of radiation was an Se electrodeless discharge lamp (EDL). The instrumental operating conditions and reagents are the same those we used in our previous paper [10].Serum samples were diluted fivefold in 0.2% v/v HNO 3 and 0.2% Triton X-100 solutions, and urine samples were diluted 1:2 v/v. After 72 h at a temperature of 100 C, feces and milk samples were weighed and digested in a sand bath heater (OVAN) with nitric acid for 72 h, and perchloric acid and chlorhydric acid (6 N) were added.…”

mentioning

confidence: 99%

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Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

Nogales

Ojeda

Delgado

et al. 2011

Journal of Reproduction and Development

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Abstract. The nutritional deficiencies provoked by ethanol consumption, during gestation or lactation, can contribute to multiple birth defects in offspring. In order to improve our knowledge about selenium (Se) distribution in pups exposed to ethanol, the present study evaluated the effect of this drug on intestinal development and determined its action on duodenal absorption of selenomethionine (Se-Met). To determinate if supplementation could improve Se absorption and its serum values, we used two antioxidant supplemented regimens on dams, with selenium alone or selenium plus folic acid, and obtained six groups of pups: C (control), A (alcohol), CS (control + Se), AS (alcohol + Se), CFS (control + Se + folic acid) and AFS (alcohol + Se + folic acid). Duodenal Se-Met transport was performed using an in vivo perfusion method. Se levels were measured by graphite furnace atomic absorption spectrometry. The supplemented diets utilized had a positive influence on body growth, duodenal perimeter and Se content in ethanol-exposed pups. Ethanol exposure increased Se-Met duodenal absorption in all pups, supplemented or not, presenting the highest values of maximal velocity (V max ) compared with their control counterparts. The affinity constant (K m ) increased according to rank: A>AS>AFS groups. These results suggest that although antioxidant supplementation does not restore Se-Met absorption to normal values, it enhances the affinity of the transporters for the substrate and improves the damage caused by ethanol in the duodenal mucosa.Key words: Ethanol, Kinetic parameters, Offspring development, Selenomethionine absorption (J. Reprod. Dev. 57: [708][709][710][711][712][713][714] 2011) T he primary function of the intestinal epithelium is to absorb the small molecules produced from food digestion [1]. However, specific substances such as alcohol could alter this intestinal absorption [2,3], producing malnutrition. Numerous studies have demonstrated that chronic ethanol consumption induces morphological and biochemical alterations in the intestinal mucosal cells [4] and that some of these changes are caused by a lipid peroxidation of duodenal membrane lipids [5]. These effects of ethanol exposure are more acute during pregnancy and lactation and can contribute to a delay in offspring growth and multiple birth defects, including fetal alcohol syndrome [6]. In this context, it is especially relevant that ethanol deregulates the homeostasis of different antioxidants such as folic acid, whose deficiency is the most common sign of malnutrition in chronic alcoholism [7]. This deficiency is associated with several alterations such as Down's syndrome and defects in neural tube closure in developing embryos [1]. According to what we have previously reported [8], the effects of ethanol exposure during gestation and lactation on folic acid intestinal absorption showed that intestinal folate absorption appears to be upregulated in suckling rats. Our research group has also found, in pups exposed to ethanol and supplemented wit...

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Alcohol, Gestation and Breastfeeding: Selenium as an Antioxidant Therapy

Cited by 42 publications

References 30 publications

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Postnatal Development of Spinal Cord and Liver Antioxidant Status in the Young of Retinol-Overdosed Female Rats

Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

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