Alcohol Induces Interdigitated Domains in Unilamellar Phosphatidylcholine Bilayers

Mou, Jianxun; Yang, Jie; Huang, Chunbo; Shao, Zongshu

doi:10.1021/bi00199a022

Cited by 164 publications

(194 citation statements)

References 20 publications

Supporting

Mentioning

167

Contrasting

Unclassified

Order By: Relevance

“…Mou et al 43 were able to induce defects into dipalmitoylphosphatidylcholine (DPPC) bilayers by fast (200 Hz) scanning at high (30 nN) forces. We could not induce such defects into our EPC/cholesterol bilayers.…”

Section: Resultsmentioning

confidence: 99%

“…Bilayers formed from vesicles that spontaneously spread on solid substrates have been described before. 42, 43 We used the following protocol: the AFM head equipped with a standard fluid cell (Digital Instruments) was mounted on freshly cleaved mica (Plano, Wetzlar, Germany) and the fluid cell was flushed with buffer 1. After 15-60 min, a 20 µm image of the mica surface was taken to ensure that it was clean.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Adsorption of Membrane-Associated Proteins to Lipid Bilayers Studied with an Atomic Force Microscope: Myelin Basic Protein and Cytochrome c

2000

View full text Add to dashboard Cite

Atomic force microscopy was used to study the structure of two membrane-associated proteins adsorbed to various supported phospholipid bilayers in physiological buffer. The aim was (a) to develop a preparation for the investigation of membrane-associated proteins at high resolution under native conditions and (b) to obtain information about the factors that determine the adsorption process and the structure of adsorbed proteins. Therefore, solid-supported membranes were formed on mica by spontaneous vesicle adsorption and spreading. Once a homogeneous, pinhole-free bilayer was formed, solutions containing the proteins at appropriate concentrations were applied. The two positively charged proteins chosen were myelin basic protein (MBP), which plays an essential role in the formation of functional myelin, and cytochrome c. On charged bilayers, MBP applied at concentrations of 0.5-50 µg/mL formed aggregates of defined height (1.9 ( 0.2 nm on negatively and 2.7 ( 0.2 nm on positively charged lipids), which at high concentration covered the entire bilayer. These aggregates are probably monomolecular layers of MBP. On neutral lipid adsorbed MBP formed irregular aggregates. Cytochrome c showed a different adsorption: On negatively charged lipid it formed aggregates of defined, monomolecular height (3.3 ( 0.2 nm). On neutral bilayers small aggregates were observed. On positively charged lipid no adsorption was observed at all. These results indicate that (a) the adsorption of cytomchrome c can be interpreted in terms of a dominating electrostatic interaction; (b) MBP adsorption to lipid bilayers is not exclusively electrostatically driven and depends on the specific lipid bilayer composition; (c) the structure of adsorbed aggregates indicates a strong protein-protein interaction.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Adsorption of Membrane-Associated Proteins to Lipid Bilayers Studied with an Atomic Force Microscope: Myelin Basic Protein and Cytochrome c

2000

View full text Add to dashboard Cite

show abstract

“…Recently, through the use of atomic force microscopy (AFM), the nm-scale structures of phospholipid films have been investigated [11,12,13,14,20,21]. While these studies revealed the lateral structures of these films (e.g., domain formation, ripple structure), most observed only one or a few layers of membranes.…”

Section: Introductionmentioning

confidence: 99%

Smooth/rough layering in liquid-crystalline/gel state of dry phospholipid film, in relation to its ability to generate giant vesicles

Hishida

Seto

Yoshikawa

2005

Chemical Physics Letters

View full text Add to dashboard Cite

Morphological changes in a dry phospholipid film on a solid substrate were studied below and above the main transition temperature, between the gel and liquidcrystalline phases by phase-contrast microscopy and AFM. A Phospholipid film in the liquid-crystalline phase exhibits flat, smooth layering, whereas that in the gel phase shows rough, random layering. These film morphologies are discussed in relation to the ability to form giant vesicles through the natural swelling method.

show abstract

“…[7][8][9][10] These lipid layers have also been useful for the biomedical research of cell recognition, membrane-mediated catalysis, anesthetic effect, and antimicrobial peptide activity. [11][12][13][14][15] The actions of the phospholipases have also been investigated using these bilayers, to study the effect of the enzymes on the wetting properties and the configuration of the bilayers, as well as the activity of the enzymes.…”

Section: 6mentioning

confidence: 99%

Effect of Vesicle Curvature on Phospholipase D Reaction-Induced-Rupture

Lee¹,

Park

2013

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

Spherical phospholipid-bilayers, vesicles, were prepared using the layer-by-layer double emulsion technique, which allows the bilayer to be formed asymmetrically. On the outer layer of the vesicles, the phospholipase D (PLD) reacted to convert phosphatidylcholine (PC) to phosphatidic acid (PA). The reaction induced the curvature change of the vesicles, which eventually led to rupture. The response time from the time of PLD injection to the time of rupture was measured against different vesicle curvatures and the outer layer phase, using the fluorescence intensity change of a pH-sensitive dye encapsulated within the vesicles. The effect of the vesicle curvature on the response was observed to be more significantly dramatic at the solid phase, compared to the liquid phase. Furthermore, in the solid phase, the response time was faster for 80 and 155 nm vesicles and, slower for 605 nm vesicles than similarly sized vesicles in the liquid phase vesicles. This difference in the response time was thought to result from the configuration determined by the phase difference and the PLD behavior.

show abstract

Alcohol Induces Interdigitated Domains in Unilamellar Phosphatidylcholine Bilayers

Cited by 164 publications

References 20 publications

Adsorption of Membrane-Associated Proteins to Lipid Bilayers Studied with an Atomic Force Microscope: Myelin Basic Protein and Cytochrome c

Adsorption of Membrane-Associated Proteins to Lipid Bilayers Studied with an Atomic Force Microscope: Myelin Basic Protein and Cytochrome c

Smooth/rough layering in liquid-crystalline/gel state of dry phospholipid film, in relation to its ability to generate giant vesicles

Effect of Vesicle Curvature on Phospholipase D Reaction-Induced-Rupture

Contact Info

Product

Resources

About