Alcohol suppresses cardiovascular diurnal variations in male normotensive rats: Role of reduced PER2 expression and CYP2E1 hyperactivity in the heart

Ethanol‐induced lung and cardiac right ventricular inflammation and remodeling underlie progression to pulmonary arterial hypertension

“…The adopted regimen resulted in a blood ethanol concentration of approximately 40 mg/ dL (Figure 1A), consistent with moderate ethanol consumption (Dasgupta, 2017). Noteworthy, the observed effects were ethanolmediated without any reduction in body weight (Figure 1D), contrary to the reduction produced by the same ethanol regimen in the Sprague-Dawley (SD) sub-strain rats in our previous study (Katary & Abdel-Rahman, 2020), suggesting sub-strain-specific effects of ethanol.…”

“…Plasma ethanol (EtOH) concentration was measured using a microplate reader (Tecan Group Ltd., Männedorf, Schweiz), where absorbance was detected at 340 nm and using a calibration curve as in our previous studies (Katary & Abdel‐Rahman, 2020; Yao & Abdel‐Rahman, 2016).…”

“…Gels were then transferred onto nitrocellulose membranes, blocked with 2.5% nonfat dry milk for 2 h, and incubated with either of the following primary antibodies: BMPR2 monoclonal antibody (Invitrogen #MA5-15827, 1:1000 dilution), ETA (Invitrogen PA3-065, 1:1000 dilution), ETB (Invitrogen PA3-066, 1:1000 dilution) receptors, β-actin (Abcam #ab115777, 1:20,000 dilution), or GAPDH (Invitrogen #MA1-16757) were used and incubated at 4°C for 24-h. Membranes were then washed in PBST and incubated with IRDye680-conjugated goat anti-mouse (1:15,000) and IRDye800conjugated goat anti-rabbit (1:15,000) from LICOR Biosciences for 1 h. Membranes were washed using PBST, and protein expression was observed (Figures S1-S3) using Odyssey M. Imager (LI-COR) and analyzed using LI-COR ImageStudio 5.2 software. Values are presented in percent of control as in our reported studies (Katary & Abdel-Rahman, 2020;Yao & Abdel-Rahman, 2016).…”

“…However, it is possible that the use of a four‐week ethanol (5% in drinking water) model hindered the progression to the PAH phenotype in this recent study. Importantly, studies that demonstrated evidence of LV injury and dysfunction utilized an eight‐week ethanol (5% in a balanced liquid diet) model (Katary & Abdel‐Rahman, 2020). Therefore, this study utilized, for the first time, the eight‐week model to investigate the long‐term effects of ethanol on cardiopulmonary function and structure.…”

Transcriptomic‐based toxicological investigations of ethanol to human umbilical vein endothelial cells

“…The relationship between ethanol and the onset of ischemic heart disease or ischemic strokes is complex: People who consume low‐to‐moderate amounts of ethanol and do not engage in irregular heavy drinking have a lower disease risk, whereas people who engage in irregular heavy drinking or who consume higher volumes of ethanol have a higher disease risk (Roerecke & Rehm, 2014). Data obtained from laboratory animals also supported that ethanol uptake induced toxicological effects to cardiovascular systems (Fathi, Nasiri, Akbari, Ahmadi‐KaniGolzar & Farajtabar, 2020; Katary & Abdel‐Rahman, 2020; Liu, 2020). Paradoxically, some studies revealed that moderate intake of red wine induced beneficial effects to animal models (De Paula et al, 2020; Liberale, Bonaventura, Montecucco, Dallegri & Carbone, 2019).…”