Alcohols and wide-bore capillaries in nonaqueous capillary electrophoresis

Porras, Simo P.; Jussila, Matti; Sinervo, Kai; Riekkola, Marja‐Liisa

doi:10.1002/(sici)1522-2683(19990801)20:12<2510::aid-elps2510>3.0.co;2-l

Cited by 27 publications

(8 citation statements)

References 23 publications

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“…The selectivity of NACE was often realized by manipulating separation medium [26], the additives and their concentrations [27], and electrolyte concentrations [28][29][30][31]. The mechanisms of NACE seem to be more complicated than those of capillary zone electrophoresis (CZE).…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Guo

Wen

Shan

et al. 2005

Journal of Chromatography A

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A method has been developed for the separation and determination of phospholipids by nonaqueous capillary electrophoresis in a separation medium of acetonitrile-2-proponol (3:2, v/v), 0.3% acetic acid and 60 mM ammonium acetate. To optimize the separation conditions, the composition of separation medium including alcohols, acetic acid, n-hexane and ammonium acetate was studied. The solvation interaction and ion-dipole interaction were also investigated. The contents of phospholipids in soybean, sunflower, peanut, apricot kernel, filbert and walnut were determined by the recommended method. The results obtained by the nonaqueous capillary electrophoreses were in good agreement with those determined by micellar electrokinetic chromatography.

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Section: Introductionmentioning

confidence: 99%

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Guo

Wen

Shan

et al. 2005

Journal of Chromatography A

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“…Similar LODs have been reported in water, MeOH and EtOH for eight catecholamines with UV detection at 214 nm 60. Riekkola and colleagues took advantage of the moderate electric current in non‐aqueous BGEs by using wider capillaries (up to 320 μm internal diameter) to enhance the UV path length, thereby increasing sensitivity 61, 62. However, problems related to EOF and solution homogeneity took place, restricting the potential of such an approach.…”

Section: Hyphenation With Detectorsmentioning

confidence: 64%

Non‐aqueous capillary electrophoresis 2005–2008

Geiser

Veuthey

2009

Electrophoresis

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This review article presents recent developments and applications of non-aqueous capillary electrophoresis (NACE): The text covers the period from the previous review (L. Geiser, J. L. Veuthey, Electrophoresis 2007, 28, 45-57) to summer 2008. We focus primarily on the analysis of pharmaceutical drugs by non-aqueous solvents in CZE within different matrices including phytochemical plant extracts and biological fluids. We also extend our discussion to other application fields (e.g. food material and environmental samples) and to chiral separations by NACE. This review focuses on practical aspects of NACE, illustrating which organic solvents and electrolytes are best suited for NACE analyses and their compatibilities with different detection techniques, including UV, LIF and MS. The review emphasizes the interests of using non-aqueous solvents in place of water for the analysis by CZE, and as an alternative to MEKC.

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“…Further applications of wide-bore capillaries in NACE [105] and approaches to suppress the siphoning [106] have also been published by Riekkola and co-workers. Although the application of NACE appears very advantageous in terms of current and heat generation at first sight, one possible difficulty needs to be mentioned.…”

Section: Nace Coupled To Mass Spectrometrymentioning

confidence: 94%

Nonaqueous capillary electrophoresis: A versatile completion of electrophoretic separation techniques

2000

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Nonaqueous capillary electrophoresis (NACE) is the application of a conductive electrolyte dissolved in either one organic solvent or a mixture of several organic solvents to carry out zone electrophoresis or related techniques in fused-silica capillaries. A complete review on the fundamentals, the optimization of analytical methods, practical considerations, and applications is given. To explain the differences to CE in aqueous media, a brief summary on solvent properties and molecular interactions in solutions introduces the reader into these fields. The use of additives to tune separation selectivity by means beyond a pure zone-electrophoretic mechanism is discussed in detail for organic media. Special detection techniques providing high potential for NACE are presented. Data on the precision of NACE methods and a list of relevant applications are included. More specialized applications like the determination of physicochemical constants in NACE or the setup of a semipreparative mode are described.

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Alcohols and wide-bore capillaries in nonaqueous capillary electrophoresis

Cited by 27 publications

References 23 publications

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Non‐aqueous capillary electrophoresis 2005–2008

Nonaqueous capillary electrophoresis: A versatile completion of electrophoretic separation techniques

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