Aldosterone determination: Comparison of a RIA assay and a CLIA assay

Belaidi, N.; Georges, A.; Brossaud, Julie; Corcuff, Jean-Benoı̂t

doi:10.1016/j.clinbiochem.2014.10.008

Cited by 10 publications

(3 citation statements)

References 7 publications

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“…Measured concentrations of aldosterone in plasma (EDTA2K) and serum samples showed high correlation (r P =0.979). The differences between pairs of plasma and serum samples ranged from 37% to 144% (median 75%) (Belaidi et al, 2015). In our study concentrations of aldosterone in serum and EDTA plasma were measured by RIA (Beckman) with correlation 0.980, while differences between pairs of plasma and serum sample were lower 5% to 85% (median 30.6%).…”

Section: Discussionmentioning

confidence: 56%

“…Most studies on PH diagnostics are focused more on optimizing ratio of aldosterone to renin or renin activity calculation, but they do not address correlation between serum and plasmatic levels. In the study by Belaidi et al (2015), who measured aldosterone in serum using RIA assay (coat-a-count, Siemens, Marburg, Germany) and CLIA using a LIAISON automated analyser (Diasorin, Saluggia, Italy) RIA and CLIA aldosterone serum concentration were linearly correlated with a slope of 0.988 and an intercept of 70.4 pmol/l. The variations of aldosterone serum concentration obtained with two assays during postural tests were very consistent.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the Chemiluminescence Immunoassay LIAISON® with the Radioimmunoassay for Aldosterone and Renin Measurement

et al. 2021

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Determination of renin plasma levels is useful in the diagnosis of hypertension and in the therapeutic follow-up of hypertensive patients. Plasmatic concentration of renin decreases in patients with hypertension due to a primary hyperaldosteronism, contrary to renovascular hypertension where concentrations of renin and aldosterone are both elevated. Blood samples (serum, EDTA plasma) were analysed using two different chemiluminiscent methods CLIA LIAISON® and radioimmunoassay for aldosterone (IMMUNOTECH Beckman Coulter) and renin (Cisbio Bioassay) measurements were compared. We used both methods to ascertain the correlation between serum vs. EDTA plasma levels of aldosterone (RIA, CLIA) and renin (IRMA, CLIA) and to compare aldosterone to renin ratios for CLIA and for radioimmunoassay: serum aldosterone to plasma renin and plasma aldosterone to plasma renin. We compared serum aldosterone CLIA vs. RIA (rP=0.933, P<0.001) and plasma renin determined using CLIA vs. IRMA (rP=0.965, P=0.062). Furthermore, we used both methods to establish the correlation between the serum vs. plasma levels of aldosterone: RIA (rP=0.980, P<0.001); CLIA (rP=0.994, P=0.353) and serum vs. plasma levels of renin: IRMA (rP=0.948, P<0.001); CLIA (rP=0.921, P=0.011). Aldosterone (serum, plasma) to plasmatic renin ratios for CLIA (rP=0.999, P=0.286) and for radioimmunoassay (rP=0.992, P=0.025). Our data demonstrate that renin and aldosterone concentrations obtained using CLIA correlate with renin and aldosterone concentrations using radioimmunoassay methods. Correlation coefficients of pair results ranged from 0.921 to 0.994. Aldosterone (serum, EDTA plasma) to plasmatic renin ratios are comparable and any of them can be used with no significant differences found.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Comparison of the Chemiluminescence Immunoassay LIAISON® with the Radioimmunoassay for Aldosterone and Renin Measurement

et al. 2021

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“…Various UALD and urine THA (UTHA) quantitative methodologies have been described. For UALD, immunoassays, including radioimmunoassay and chemiluminescent immunoassay, are commonly used (Belaidi et al, 2015; Derlet et al, 2014; Ekins et al, 1972). A few laboratories measured UALD using GC–MS (Külpmann et al, 1973; Leung & Griffiths, 1972) or LC–MS/MS (Bekkach et al, 2016; Yin et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC–MS/MS method

Cheng,

Lo,

Chan

et al. 2023

Biomedical Chromatography

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Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC–MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC–MS/MS. The analytical performance of this method was evaluated. The within‐batch and between‐batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at −80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20–65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2–38 and 9–139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism.

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